Applications of plant growth promoters influenced plants growth and have a quicker effect on vegetative growth as well as flower yield of flowering crops. That’s why an experiment was accomplished in the Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh to observe the effect of the growth promoters on Gerbera cultivars. Fourteen gerbera cultivars viz. V1= Sweet pink with black centre, V2= Yellow with black centre, V3= Reddish orange with black centre, V4= Red with yellow centre, V5= White with yellow centre, V6= Purplish pink with yellow centre, V7= Yellow, V8= Creamy yellow with yellow centre, V9= Magenta with black centre, V10= Red with black centre, V11= Bright orange with yellow centre, V12= Magenta pink with black centre, V13= Orange with yellow centre, V14= Yellow with greenish centre were used as factor A in this experiment. Factor B was growth chemicals denoted as T0 (control), T1 (4-CPA), T2 (Flora) and T3 (GA3). This experiment was organised in Randomized Complete Block Design with three replications. In this study, V6 showed the highest head diameter but V1 showed the highest flower head diameter when treated with GA3 (T3). Again in all other cases, V1 showed the best performance and among the growth chemicals GA3 (T3) was observed best, Flora (T2) showed better performance than 4-CPA (T1).