Foliar application of glutamate and phenylalanine induced regulation in yield, protein components, aroma, and metabolites in fragrant rice

Luo, Haowen; Imran, Muhammad; Yao, Xiang-Bin; Zhang, Simin; Wang, Yi; Xing, Pipeng; Tang, Xiangru

doi:10.1016/j.eja.2023.126899

Cited by 6 publications

(1 citation statement)

References 33 publications

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“…The determination of 2-AP was carried out according to the methods described by previous studies. , The ground samples were extracted with dichloromethane for 4 h. Then, the supernatant was transferred to a vial with a disposable micropipette and placed in a GCMS-QP2010 machine (Shimadzu Corporation, Japan) for GC-MS analysis. The 2-AP content was expressed as μg kg –1 .…”

Section: Methodsmentioning

confidence: 99%

Regulation of 2-Acetyl-1-pyrroline Content in Fragrant Rice under Different Temperatures at the Grain-Filling Stage

Luo,

Zhang,

Lai

et al. 2024

J. Agric. Food Chem.

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2-Acetyl-1-pyrroline (2-AP) is a key volatile organic compound in fragrant rice aroma. However, the effects of temperature on 2-AP biosynthesis in fragrant rice and its regulation mechanism have been rarely reported. In the present study, three fragrant rice varieties were used as plant materials, and four temperature treatments during the grain-filling stage, i.e., (T1) 22/17 °C, (T2) 27/22 °C, (T3) 32/27 °C, and (T4) 37/32 °C, were adopted. The results showed that grain contents of 2-AP, proline, and γaminobutyric acid (GABA) significantly (P < 0.05) increased with decreased temperature, while the lowest and highest 2-AP contents were recorded in the T4 and T1 treatments, respectively. Higher pyrroline-5-carboxylic acid (P5C) content was recorded in low-temperature treatments (T1 and T2) than in high-temperature treatments (T3 and T4). The transcript levels of genes BADH2, PRODH, and OAT significantly (P < 0.05) decreased with decreased temperature. Lower transcript levels of genes P5CR, P5CS2, DAO2, DAO4, and DAO5 were recorded in low-temperature treatments (T1 and T2) than in high-temperature treatments (T3 and T4). In conclusion, low temperature increased 2-AP content and high temperature decreased 2-AP content in fragrant rice. We deduced that temperature regulated 2-AP biosynthesis through the metabolism of proline and GABA.

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Section: Methodsmentioning

confidence: 99%