Foliar Regeneration in Anthurium andraeanum Hort. cv. Agnihothri

Bejoy, M.; Sumitha, V. R.; Anish, N.P.

doi:10.3923/biotech.2008.134.138

Cited by 20 publications

(17 citation statements)

References 16 publications

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“…The application of anther culture for producing homozygous double-haploid (DH) lines of Anthurium can be of great importance (Maluszynski et al 2003) because of its high frequency of cross-pollination, high heterozygosity in seed-derived progenies (Geier 1990;Callotte 2004;Dufour and Guérin 2006), and long growth period of plants after pollination (Higaki et al 1995;Bejoy et al 2008;Jahan et al 2009). Anther culture of anthurium was first attempted by Custers (2004) by culturing the whole anther and its filament on half-strength MS medium (Murashige and Skoog 1962) 6-benzyladenine (BA) and 0.54 lM a-naphthaleneacetic acid (NAA), but without any positive results.…”

Section: Introductionmentioning

confidence: 98%

Anther culture of Anthurium: a review

Silva

Winarto²,

Dobránszki

et al. 2015

Acta Physiol Plant

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The use of anther culture to produce haploid plants is a useful breeding technique for Anthurium, but has only seen limited applications thus far. This review describes the advances achieved thus far, and provides practical tips and protocols that would allow researchers new to the field, or already experienced Anthurium tissue culture scientists, to establish a new field of research, or a novel way to derive haploid plants for breeding programs. Anther culture can result in considerable somaclonal variation, but this has translated into a novel way of inducing new color variants of the spathe, the most marketable part of this ornamental plant. Considering this variability in vitro, anther culture is most likely not the most suitable method for clonal propagation of important germplasm, but is certainly a viable method to induce new genetically stable leaf and flower color variants.

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Section: Introductionmentioning

confidence: 98%

Anther culture of Anthurium: a review

Silva

Winarto²,

Dobránszki

et al. 2015

Acta Physiol Plant

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“…Furthermore, propagation via seeds produce heterogeneous progenies attributable to cross pollination (Bejoy et al 2008), resulting in disparity in the form of colour, quality, yield and the time to first flowering ). In addition, the seeds have been reported to be viable only for 2-3 days after harvest with very low germination of 20-30 % ).…”

Section: Introductionmentioning

confidence: 99%

Direct induction of protocorm-like bodies from shoot tips, plantlet formation, and clonal fidelity analysis in Anthurium andreanum cv. CanCan

et al. 2012

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Protocorm-like bodies (PLBs) were induced directly at high frequency from wounded surface of Anthurium andreanum cv. CanCan shoot tip-ends, used as explants. In order to obtain PLB directly, the influence of different types and concentrations of cytokinins were evaluated. Amid the cytokinins, N 6 -(D 2 -isopentenyl)-adenine (2-iP) at a concentration of 15 lM was most effective in inducing PLB whereby *98 (97.8) % of explants induced PLB with an average of 120 PLBs per shoot tip within 50 days of culture. Stereomicroscopic observation meticulously revealed the sequential changes from initiation to maturation of PLB gradually forming shoot apical meristem, shoot primordia and leaf primordia. Mature PLBs showed significant shoot proliferation (98.4 %) in media containing 10 lM 6-furfurylaminopurine forming 17 shoots per PLB within 30 days. The inclusion of activated charcoal (AC) in media containing auxin had promotive effect on rooting whereby 5 lM indole-3-butyric acid plus 500 lM AC resulted in highest number and length of roots. Successfully acclimatized plants, subjected to random amplified polymorphic DNA assessment for genetic fidelity, did not show any variation. Thus, this complete study has successfully outlined a rapid, high frequency direct induction of PLB of Anthurium from shoot tips inclusive of shoot proliferation, rooting and acclimatization.

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“…In vitro plant propagation in Anthurium has been achieved by using various tissues including leaf, petiole, spadix, spathe, seed, lateral bud and shoot tips through direct and indirect organogenesis (Atak and Celik, 2009) and this technique is commercially used (Martin et al, 2003) for production of planting material. Further, various physical and biological factors including media play role during Anthurium micro propagation (Silva et al, 2005;Bejoy et al, 2008) and one of the important factors among them was genotype. However, reports on reliable mass multiplication system through long term in vitro culture and commercially viable systems are lacking.…”

Section: Issn: 2319-7706 Volume 6 Number 9 (2017) Pp 2579-2584mentioning

confidence: 99%