Foliar regeneration of the endangered Red Vanda, Renanthera imschootiana Rolfe (Orchidaceae)

Abstract

An in vitro method was developed to regenerate large numbers of phenotypically uniform plants from the basal parts of the leaves of flowering plants of Renanthera imschootiana Rolfe. Differentiation of up to 10 shoot buds free of callus and protocorm-like bodies occurred in 10-12 weeks from the base of a single leaf implanted in Mitra et al. (1976) medium supplemented with 2% sucrose, 2gl -l peptone, 44.4 txM benzyladenine (BA) and 10.7 I~M naphthaleneacetic acid (NAA). Subculture of the tissues in medium enriched with 10% coconut water and 35 g 1 i ripe banana pulp resulted in the production of highest average number of 40 shoots in 12 weeks. No difference in the regeneration potential was observed among the three young leaves while mature leaves did not respond. All the leaves of the regenerated shoots were easily recultured to increase shoot multiplication. Shoots readily formed roots on transfer to a medium containing 4.41xM BA, 10.7 IxM NAA and 1% activated charcoal. All regenerated plants examined were normal diploids with 2n = 38. Foliar meristem culture appears to have great potential for ex situ conservation and propagation of this extremely endangered orchid.

Embryogenic Callus Induction of Pencil Orchid (Papilionanthe hookeriana Rchb.f.) Through in Vitro Culture

“…This study is also in line with research conducted by [17] on the Vanda spatula orchid which is a specific Sri Lanka's orchid and has a very beautiful flower. Callus growth from the flower stalk was produced after 6 months.…”

“…The highest number of shoot (70 -100 shoots per explant) had pruced on medium Mira enriched with 35 g L -1 banana pulp, 30% coconut water and 1,08 µM NAA after 30 weeks of culture [25]. The endangered Blue Vanda is specific Vanda from located in Sri Lanka that has a beautiful flower (The IUCN Red List of threatened species too) [17].…”

In-Vitro Seed Germination and Effect of Growth Regulators on Subsequent Development of Protocorms of Eulophia Nuda Lindl

“…The enhancing effects of cytokinins on morphogenesis were previously reported (Goh & Wong, 1990). The differentiation of multiple shoots from corm and/or protocorm like bodies (PLBs) have been reported in Renanthera imschootiana (Seeni and Latha, 1992); Cymbidium aloifolium (Kaur and Sharma, 1997;Bujarbarua and Sharma, 1997); Dendrobium aphyllum (Talukdar, 2001) Habeneria marginata (Sheelavantmath and Murthy, 2001) and Eulophia nuda (Panwar et al 2012).…”

A Review on: In Vitro Cloning of Orchids

“…Wimber (1965) first used Cymbidium leaf tissue culture for PLBs production. Seeni and Latha, 1992 successfully regenerated endangered Renanthera imschootiana Rolfe (Red Vanda) from leaf tissue culture. The direct adventive shoot bud formation was observed in the bases of the leaves.…”