Folic acid-conjugated soybean protein-based nanoparticles mediate efficient antitumor ability in vitro

Abstract

In this study, soy protein isolate was hydrolyzed by compound enzymes to give aqueous soy protein with low molecular weights. Folic acid modified and free soy protein nanoparticles were successfully prepared by a desolvation method as target-specific drug delivery, respectively. Ultraviolet spectrophotometry demonstrated that folic acid was successfully grafted onto soy protein. The shape and size of folic acid modified soy protein nanoparticles were detected by transmission electron microscopy, scanning electron microscope, and dynamic light scattering. In addition, a series of characteristics including kinetic stability, pH stability, and time stability were also performed. Doxorubicin was successfully loaded into folic acid modified soy protein nanoparticles, and the encapsulation and loading efficiencies were 96.7% and 23%, respectively. Doxorubicin-loaded folic acid modified soy protein nanoparticles exhibited faster drug release rate than soy protein nanoparticles in PBS solution (pH = 5). The tumor penetration and antitumor experiments were done using three-dimensional multicellular tumor spheroids as the in vitro model. The results proved that folic acid modified soy protein nanoparticles display higher penetration and accumulation than soy protein nanoparticles, therefore possessing efficient growth inhibitory ability against multicellular tumor spheroids.

Gliadin Nanoparticles Containing Doxorubicin Hydrochloride: Characterization and Cytotoxicity

“…The LC of the samples confirm this trend and showed increasing values confirming the ability of the polymeric matrix to hold the drug. These profiles can result from non-covalent bonds between the protonated amine group of DOX and the negatively-charged carboxyl groups of the polymer, as already described for several other protein-based DOX-loaded nanoformulations [ 49 , 50 , 51 , 52 , 53 ].…”

pH and magnetic field responsive protein‐inorganic nanohybrid conjugated with biotin: A biocompatible carrier system targeting lung cancer cells

“…Targeting ligand biotin was conjugated onto drug encapsulated casein and casein‐CFNP hybrid applying the EDC coupling reaction 35 . BT was dissolved in DMSO at a concentration of 2 mg/ml and the solution was activated by adding EDC.…”

Antitumor Features of Vegetal Protein-Based Nanotherapeutics

“…Successively, these nanovectors were conjugated to folic acid, showing peculiar targeting properties for human colon carcinoma cells [157]. The same approach was used by other research teams in order to deliver doxorubicin, increasing its cytotoxicity activity against SH-SY5Y, MCF-7 and 293 T cells [158].…”