Folic acid prevents habituation memory impairment and oxidative stress in an aging model induced by D-galactose

Garcez, Michelle Lima; Cassoma, Ricardo Chiengo Sapalo; Mina, Francielle; Bellettini-Santos, Tatiani; Amparo, Luz; Schiavo, Gustavo Luis; Medeiros, Eduarda Behenck; Campos, Ana Carolina Brunatto Falchetti; Silva, Sabrina da; Rempel, Lisienny Campoli Tono; Steckert, Amanda V.; Barichello, Tatiana; Budni, Josiane

doi:10.1007/s11011-020-00647-7

Cited by 13 publications

(5 citation statements)

References 83 publications

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“…This study was approved by the Ethics Committee of The Third Affiliated Hospital of Xi'an Medical University (XAMU2022-05) and conducted under the approved guidelines. To explore the effect of naringin on cognitive dysfunction in aging rats, we constructed an aging rat model of cognitive dysfunction by subcutaneous injection of D-gal (150 mg/kg) following the method in the study by Garcez et al [ 18 ]. Twenty Wistar rats (weight: 220 ± 20 g) were randomly divided into 4 groups ( n = 5).…”

Section: Methodsmentioning

confidence: 99%

Naringin Prevents Cognitive Dysfunction in Aging Rats by Inhibiting Toll‐Like Receptor 4 (TLR4)/NF‐κB Pathway and Endoplasmic Reticulum Stress

Dai¹,

Jia²,

Cao

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Objective. Naringin is a flavonoid derived from Chinese herbs. According to earlier studies, naringin may have the potential to alleviate aging-induced cognitive dysfunction. Therefore, this study attempted to explore the protective effect and underlying mechanism of naringin on aging rats with cognitive dysfunction. Methods. After the construction of a model of aging rats with cognitive dysfunction through subcutaneous injection of D-galactose (D-gal; 150 mg/kg), intragastric administration of naringin (100 mg/kg) was performed for treatment. Behavioral tests, including Morris water maze test (MWM), novel object recognition test (NORT), and fear conditioning test, were used to measure the cognitive function; ELISA and biochemical tests were used to determine the levels of interleukin (IL)-1β, IL-6, monocyte chemoattractant protein-1 (MCP-1), brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) in the hippocampus of rats in each group, respectively; H&E staining was used to observe the pathological changes in the hippocampus; Western blot was used to examine the expression of toll-like receptor 4 (TLR4)/NF-κB pathway-related proteins and endoplasmic reticulum (ER) stress-related proteins in the hippocampus. Results. The model was successfully constructed by subcutaneous injection of D-gal (150 mg/kg). The behavioral test results showed that naringin could ameliorate the cognitive dysfunction and alleviate the histopathological damage of hippocampus. Moreover, naringin significantly improve the inflammatory response (the levels of IL-1β, IL-6, and MCP-1 were decreased), oxidative stress response (MDA level was increased while GSH-Px activity was decreased), and ER stress (the expression of glucose-regulated protein 78 (GRP78), C/-EBP homologous protein (CHOP), and transcription factor 6 (ATF6) expression was downregulated), and increased the levels of neurotrophic factors BDNF and NGF in D-gal rats. Besides, further mechanistic studies revealed the downregulation of naringin on TLR4/NF-κB pathway activity. Conclusion. Naringin may inhibit inflammatory response, oxidative stress, and ER stress by downregulating TLR4/NF-κB pathway activity, thereby improving cognitive dysfunction and alleviating histopathological damage of hippocampus in aging rats. Briefly, naringin is an effective drug for the treatment of cognitive dysfunction.

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Section: Methodsmentioning

confidence: 99%

Naringin Prevents Cognitive Dysfunction in Aging Rats by Inhibiting Toll‐Like Receptor 4 (TLR4)/NF‐κB Pathway and Endoplasmic Reticulum Stress

Dai¹,

Jia²,

Cao

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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“…Exercise ameliorated decreased expression of Syp in hippocampus and could significantly improve the learning and memory capacity in aging process (Chen et al, 2020). The same phenomenon was observed in the hippocampus of aging mice induced by D-galactose (Li et al, 2019;Garcez et al, 2021). Despite Syp plays an important role in memory impediment and progression to aging, the mechanisms that regulate its expression, and contribute to its increased expression in hippocampus, are not well understood.…”

Section: Introductionmentioning

confidence: 68%

Circ-Vps41 positively modulates Syp and its overexpression improves memory ability in aging mice

Wang

Gao

et al. 2022

Front. Mol. Neurosci.

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IntroductionAge is an established risk factor for neurodegenerative disorders. Aging-related cognitive decline is a common cause of memory impairment in aging individuals, in which hippocampal synaptic plasticity and hippocampus-dependent memory formation are damaged. Circular RNAs (circRNAs) have been reported in many cognitive disorders, but their role in aging-related memory impairment is unclear.Methods: In this study, we aimed to investigate the effects of circ-Vps41 on aging-related hippocampus-dependent memory impairment and explore the potential mechanisms. Here, D-galactose was used to produce a conventional aging model resulting in memory dysfunction.ResultsCirc-Vps41 was significantly downregulated in D-galactose-induced aging in vitro and in vivo. The overexpression of circ-Vps41 could upregulate synaptophysin (Syp), thereby promoting the synaptic plasticity and alleviating cognitive impairment in aging mice. Mechanistically, we found that circ-Vps41 upregulated Syp expression by physically binding to miR-24-3p. Moreover, the miR-24-3p mimics reversed the circ-Vps41 overexpression-induced increase in Syp expression.DiscussionOverexpression of circ-Vps41 alleviated the synaptic plasticity and memory dysfunction via the miR-24-3p/Syp axis. These findings revealed circ-Vps41 regulatory network and provided new insights into its potential mechanisms for improving aging-related learning and memory impairment.

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“…73 d -gal decreased synaptophysin in the rat brain, reflecting the efficiency of transmission of neurological signals between different neurons and the general weakness of animals. 65,74–76 Also, Budni et al 77 observed reductions in synaptophysin and TAU protein levels in the prefrontal cortex in the d -gal-treated rats.…”

Section: Discussionmentioning

confidence: 97%

Rutin attenuates d-galactose-induced oxidative stress in rats’ brain and liver: molecular docking and experimental approaches

et al. 2023

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Folic acid prevents habituation memory impairment and oxidative stress in an aging model induced by D-galactose

Cited by 13 publications

References 83 publications

Naringin Prevents Cognitive Dysfunction in Aging Rats by Inhibiting Toll‐Like Receptor 4 (TLR4)/NF‐κB Pathway and Endoplasmic Reticulum Stress

Naringin Prevents Cognitive Dysfunction in Aging Rats by Inhibiting Toll‐Like Receptor 4 (TLR4)/NF‐κB Pathway and Endoplasmic Reticulum Stress

Circ-Vps41 positively modulates Syp and its overexpression improves memory ability in aging mice

Rutin attenuates d-galactose-induced oxidative stress in rats’ brain and liver: molecular docking and experimental approaches

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