Follicular fluid steroid levels in relation to oocyte maturity and in vitro fertilization

Vanluchene, E.; Hinting, Aucky; Dhont, Marc; Sutter, Petra De; Maele, Georges Van; Vandekerckhove, D

doi:10.1016/0960-0760(91)90405-t

Cited by 16 publications

(6 citation statements)

References 17 publications

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“…Several investigators have demonstrated that mature oocytes with a higher potential for fertilization are also associated with lower testosterone levels in FF at the time of follicular rupture (20,21). Our results are in agreement with these data.…”

Section: Discussionsupporting

confidence: 83%

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Costa

Mendes

Ferriani

et al. 2004

Braz J Med Biol Res

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The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing mature oocytes (median = 368 and 5.7 ng/ml, respectively; P < 0.05). Progesterone, androstenedione and estrone levels were not significantly different between mature and immature oocytes. The application of the receiver-operating characteristic curve statistical approach to determine the best cut-off point for the discrimination between mature and immature oocytes indicated levels of 505.8 ng/ml for E2 (81.0% sensitivity and 81.8% specificity) and of 10.4 ng/ ml for testosterone (90.9% sensitivity and 82.4% specificity). Follicular diameter was associated negatively with E2 and testosterone levels in FF. There was a significant increase in progesterone/testosterone, progesterone/E2 and E2/testosterone ratios in FF containing mature oocytes, suggesting a reduction in conversion of C21 to C19, but not in aromatase activity. The overall fertility rate was 61% but there was no correlation between the steroid levels or their ratios and the fertilization rates. E2 and testosterone levels in FF may be used as a predictive parameter of oocyte maturity, but not for the in vitro fertilization rate.

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Section: Discussionsupporting

confidence: 83%

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Costa

Mendes

Ferriani

et al. 2004

Braz J Med Biol Res

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show abstract

“…Several studies have shown that mature oocytes with a higher potential for fertilization are also associated with lower testosterone levels in follicular fluid at the time of follicular rupture (Brailly et al, 1981;Vanluchene et al, 1991;Costa et al, 2004). Our results are in agreement with these data.…”

Section: Discussionsupporting

confidence: 93%

Interleukin 6, interleukin 1b, estradiol and testosterone concentrations in serum and follicular fluid of females with stimulated and non-stimulated ovaries

Sheikh¹,

Amiri²,

Farimani³

et al. 2011

Afr. J. Biotechnol.

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The ovarian physiology is regulated by some complex factors such as hormones and growth factors. The hormones and growth factors are synthesized by follicular and ovarian cells during follicular maturation stage and cytokines are synthesized by the immune system. Interaction between immune and endocrine systems modulates ovarian function through the secretion of regulatory soluble factors, especially cytokines. There is a close contact between corona-cumulus-oocyte complex with follicular fluid which affects the quality and degree of oocyte maturation. The objectives of this study were to determine the levels of interleukin-6 (IL-6), interleukin 1β β β β, estradiol and testosterone concentration in serum and follicular fluid of women with stimulated and non-stimulated ovaries. A total of 41 women, 27 in stimulated and 14 non-stimulated cycles, undergoing intra cytoplasmic sperm injection (ICSI) treatment were included in this study. Follicular fluid and serum samples from all cases were collected at the time of oocyte retrieval and concentration of testosterone, estradiol, interleukin 6 and interleukin 1β β β β were measured. The results show that serum concentration of testosterone was significantly higher in non stimulated cases. Also, the serum and follicular fluid concentrations of interleukin 6 and interleukin 1β β β β in stimulated cases were significantly higher than non stimulated group. There was an important negative correlation between the level of testosterone in patient's serum and IL-6 and IL-1β β β β levels of follicular fluid. In conclusion, according to these results, it seems that the levels of testosterone and IL-6 and IL-1β β β β in patient's serum and follicular fluid are a good factor for prediction of maturity of oocytes.

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“…However, the attraction of bull spermatozoa was later shown to be the result of adhesion to glass [11], and the attraction of human spermatozoa was not controlled for the possibilities of trapping and/or chemokinesis (in this context trapping may involve adsorption of spermatozoa or a negative effect of the "attractant" on the sperm motility). Follicular fluids include the secretions of the egg and its surrounding cells, have a large number of bioactive substances [12][13][14], and have been shown to affect sperm physiology and motility (though with apparently conflicting observations) [4,[15][16][17][18]. Attraction of human spermatozoa to follicular fluid, correlated with egg fertilization, was indeed demonstrated, but the issue of whether or not the attraction was due to chemotaxis was not determined [19].…”

Section: Introductionmentioning

confidence: 93%

Chemotaxis and Chemokinesis of Human Spermatozoa to Follicular Factors1

Ralt

Manor

Cohen-Dayag

et al. 1994

Biology of Reproduction

134

122

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Human spermatozoa accumulate in vitro in diluted follicular fluids obtained from follicles from which the eggs have been fertilized. Using capillary assays under a variety of experimental conditions (ascending or descending gradients of follicular fluid, or no gradient at all) and microscopic assays in which individual spermatozoa could be followed, we found that the sperm accumulation in follicular fluid was the result of both sperm chemotaxis and chemokinesis and eventually hyperactivation-like motility. We determined the optimal conditions for sperm accumulation, which involved sperm preincubation (possibly to induce sperm capacitation) and proper dilution of follicular fluid. In all the assays, the net accumulation was low, probably reflecting the chemotactic responsiveness of only a small fraction of the sperm population at any given time. We partially fractionated follicular fluid in a Centricon microconcentrator (Amicon, Danvers, MA) and by acetone precipitation, and found that at least one of the chemotactic factors is a small (< 10-kDa) molecule that is probably nonhydrophobic. This is the first time that sperm chemotaxis and chemokinesis in response to a follicular factor(s) in mammals has been established and has been distinguished from other processes that might cause sperm accumulation. The physiological significance of these findings is discussed.

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Follicular fluid steroid levels in relation to oocyte maturity and in vitro fertilization

Cited by 16 publications

References 17 publications

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Interleukin 6, interleukin 1b, estradiol and testosterone concentrations in serum and follicular fluid of females with stimulated and non-stimulated ovaries

Chemotaxis and Chemokinesis of Human Spermatozoa to Follicular Factors1

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