Follicular populations, recruitment and atresia in the ovaries of different strains of mice

Campos-Júnior, Paulo Henrique Almeida; Assunção, C. M.; Carvalho, Bruno Campos de; Batista, Ríbrio Ivan Tavares Pereira; García, Raúl; Viana, J. H. M.

doi:10.1016/s1642-431x(12)60076-x

Cited by 15 publications

(17 citation statements)

References 28 publications

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“…According to Pepling et al, different numbers of follicles in mouse ovaries could be due to methods used to assess their number, leading to differences between animals of the same age and genetic strain [ 73 ]. More recently, differences in primordial follicle population among different mice strains was demonstrated to be attenuated by different patterns of follicular recruitment and atresia [ 74 ]. Nevertheless, in our study, each experiment was performed with its own mice strain control and altogether the efficiency of inhibitors such as LY294002 and rapamycin was confirmed to limit follicular activation either by in vitro analysis of signalling pathways or by immunohistochemistry and follicular counting both in mature and juvenile ovaries.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture

2021

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Background Cryopreservation and transplantation of ovarian tissue (OTCTP) represent a promising fertility preservation technique for prepubertal patients or for patients requiring urgent oncological management. However, a major obstacle of this technique is follicle loss due to, among others, accelerated recruitment of primordial follicles during the transplantation process, leading to follicular reserve loss in the graft and thereby potentially reducing its lifespan. This study aimed to assess how cryopreservation itself impacts follicle activation. Results Western blot analysis of the PI3K/PTEN/Akt and mTOR signalling pathways showed that they were activated in mature or juvenile slow-frozen murine ovaries compared to control fresh ovaries. The use of pharmacological inhibitors of follicle signalling pathways during the cryopreservation process decreased cryopreservation-induced follicle recruitment. The second aim of this study was to use in vitro organotypic culture of cryopreserved ovaries and to test pharmacological inhibitors of the PI3K/PTEN/Akt and mTOR pathways. In vitro organotypic culture-induced activation of the PI3K/PTEN/Akt pathway is counteracted by cryopreservation with rapamycin and in vitro culture in the presence of LY294002. These results were confirmed by follicle density quantifications. Indeed, follicle development is affected by in vitro organotypic culture, and PI3K/PTEN/Akt and mTOR pharmacological inhibitors preserve primordial follicle reserve. Conclusions Our findings support the hypothesis that inhibitors of mTOR and PI3K might be an attractive tool to delay primordial follicle activation induced by cryopreservation and culture, thus preserving the ovarian reserve while retaining follicles in a functionally integrated state.

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Section: Discussionmentioning

confidence: 99%

Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture

2021

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“…Embedded grafts were serially sliced (5 μm) for a total of 40 sections per graft. The slices were stained with hematoxylin/eosin and were qualitatively examined for the presence of ovarian follicles (primordial, primary, secondary, antral, and atretic), which were classified as previously described [ 19 ]. Additionally, the volume densities of the graft histological components were determined by images captured with light microscopy, using a 540-intersection grid from ImageJ software (National Institutes of Health, http://rsb.info.nih.gov/ij/ ).…”

Section: Methodsmentioning

confidence: 99%

Ovarian Grafts 10 Days after Xenotransplantation: Folliculogenesis and Recovery of Viable Oocytes

et al. 2016

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Ovarian xenotransplantation is a promising alternative to preserve fertility of oncologic patients. However, several functional aspects of this procedure remained to be addressed. The aim of this study was evaluate the feasibility of xenotransplantation as a strategy to maintain bovine ovarian grafts and produce oocytes. Adult ovarian cortical pieces were xenotransplanted to the dorsal subcutaneous of female NOD-SCID mice (n = 62). Grafts were recovered ten days after xenotransplantation. Host and graft weights; folliculogenesis progression; blood perfusion, relative gene expression and number of macrophage and neutrophil of xenografts; in vitro developmental competence of graft-derived oocytes were evaluated. Folliculogenesis was supported in the grafts, as indicated by the presence of primordial, primary, secondary, antral, and atretic follicles. The xenografts showed a greater volumetric density of atretic follicles and higher hyperemia and number of host-derived macrophage and neutrophil (P<0.05), when compared to non-grafted fragments. There was a higher blood perfusion under the back skin in the transplantation sites of host animals than in control and non-grafted (P<0.01). BAX and PRDX1 genes were up-regulated, while BCL2, FSHR, IGF1R and IGF2R were down-regulated, when compared to the control (P<0.01). Twenty seven oocytes were successfully harvested from grafts, and some of these oocytes were able to give rise to blastocysts after in vitro fertilization. However, cleavage and blastocyst rates of xenograft derived oocytes were lower than in control (P<0.01). Despite showing some functional modifications, the ovarian xenografts were able to support folliculogenesis and produce functional oocytes.

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“…In every fifty sections, the number of primordial, primary, secondary, antral, and atretic follicles were quantified, estimating the total number of follicles per ovary. Follicles were classified and counted [16] and follicles (from primordial to antral) showing morphological signs of death such as pyknosis, cellular fragmentation, and disintegration were classified as atretic [16]. Only follicles containing an oocyte with a visible nucleus were considered to avoid double-counting.…”

Section: Ovarian Follicular Quantificationmentioning

confidence: 99%

“…Results are shown as the number of counted follicles per animal. Atresia rate (%) was determined as the number of atretic follicles/total number of follicles*100; and activation rate (%) as the number of growing follicles/ total number of follicles*100 [16].…”

Section: Ovarian Follicular Quantificationmentioning

confidence: 99%

Gastric administration of Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic during the pregestational and gestational periods does not influence the follicular endowment of the progeny

Freitas

Lopes

Nascimento

et al. 2022

Preprint

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Fetal programming suggests that maternal stimulation and nutrition during the period of fetal development can program the progeny. Conjugated linoleic acid (CLA), an isomer of linoleic acid, has been characterized in several aspects, but few studies have been performed on its involvement in reproduction and fetal programming. The aim of this study was to evaluate the F1, F2 and F3 progeny of female mice supplemented with CLA during the pregestational and gestational periods with respect to biometric and reproductive parameters, as well as ovarian morphophysiology. The F1 progeny of mothers supplemented with CLA exhibited stable weight gain, while the F2 progeny showed no effects (P=0.0187 and P=0.0245, respectively). A reduction in Lee's Index was observed in both generations at the second post-weaning evaluation week in the animals treated with CLA (P=0.0100 and P=0.0078, respectively). The F2 generation showed an increase in the anogenital index in both sexes of the animals treated with CLA (P= 0.0114 and P<0.0001, female and male respectively). CLA administration to mothers did not affect any of the following in their progeny: ovarian follicle mobilization (P>0.05), follicle number (P>0.05) and the integrated density of the lipid content of oocytes included in antral follicles (P>0.05). This study evaluated the use of CLA in mothers and found that it did not affect the progeny regarding murine reproductive performance, suggesting that this supplement can be used safely.

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Follicular populations, recruitment and atresia in the ovaries of different strains of mice

Cited by 15 publications

References 28 publications

Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture

Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture

Ovarian Grafts 10 Days after Xenotransplantation: Folliculogenesis and Recovery of Viable Oocytes

Gastric administration of Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic during the pregestational and gestational periods does not influence the follicular endowment of the progeny

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