Folliculogenesis-related genes are differently expressed in secondary and tertiary ovarian follicles

Abstract

Summary The relative mRNA abundance of 10 genes associated with folliculogenesis was compared between late preantral (secondary) and early antral (tertiary) ovarian follicles in goats. In total, 100 follicles in each category were mechanically isolated. The relative transcript abundance of the mRNAs were determined by qPCR. Data were analyzed using unpaired Student’s t-test. Of the 10 tested genes, ABLIM mRNA was not detected in either follicle category, six genes (SLIT3, TYMS, GTPBP1, AKR1C4, PIK3R6, and MAOB) were upregulated in secondary follicles compared with tertiary follicles, and three genes (ARHGEF12, CLEC6A, and CYTL1) showed similar mRNA abundances in both secondary and tertiary follicles. In conclusion, SLIT3, GTPBP1, AKR1C4, and PIK3R6 mRNA abundance was upregulated in secondary follicles (preantral phase) compared with in tertiary follicles (antral phase) in goats.

Transcriptomic and metabolomic analyses of the ovaries of Taihe black-bone silky fowls at the peak egg-laying and nesting period

“…In the ovary, elevated concentrations of progesterone or estradiol lead to enhanced CYTL1 expression; in the uterus, CYTL1 expression is significantly enhanced in endometrial cells with increasing concentrations of progesterone and estrogen, suggesting that CYTL1 is a candidate marker of endometrial tolerance and that upregulation of CYTL1 leads to significant proliferation of endometrial cells ( Ai et al, 2016 ). In addition, it has been reported that CYTL1 can mediate the regulation of different stages of folliculogenesis ( Moura et al, 2021 ). The tolerance of the endometrium is important for the reproduction of offspring in females, in the study, we hypothesized that the high expression of this gene during the PP contributes to ovarian maintenance and has an important role in egg production.…”

Narrow-Headed Voles Species Complex (Cricetidae, Rodentia): Evidence for Species Differentiation Inferred from Transcriptome Data

“…The relaxation of selection was demonstrated with CST3, DNAJB4, DSG2, MRTO4, MFSD14A, NOSTRIN, PSPC1, and VWA8, which were potentially involved in various aspects of spermatogenesis [53,[79][80][81][82][83][84][85]; ITGB5, which played a role in male fertility [86][87][88]; IPO13, which was involved in meiosis of germ cells [89]; and GTPBP1, MRPS5, and PSPC1, which were involved in oogenesis [90][91][92]. CST3 regulated proteolysis in the male reproductive system [93], NOSTRIN was involved in placenta development [94], CA4 and ITM2B were differentially expressed in female and male reproductive tissues [95][96][97][98].…”