Follow-Up of Osteochondral Plug Transfers in a Goat Model

Lane, Joseph R.; Massie, Jennifer B.; Ball, Scott T.; Amiel, Michael E.; Chen, Albert C.; Bae, Won C.; Sah, Robert L.; Amiel, David

doi:10.1177/0363546504263945

Cited by 128 publications

(105 citation statements)

References 35 publications

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“…Full integration has been reported in long-term experiments (Petersen et al 2008), whereas others reported a cleft between the recipient articular cartilage and osteochondral plugs in goats and human (Lane et al 2004;Horas et al 2003). Our study suggests that these results may reflect a difference in the total content of collagen in the implants, which is important for the interface shear strains.…”

Section: Discussionsupporting

confidence: 65%

The effect of tissue-engineered cartilage biomechanical and biochemical properties on its post-implantation mechanical behavior

Khoshgoftar

Wilson

Ito

et al. 2012

Biomech Model Mechanobiol

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The insufficient load-bearing capacity of today's tissue-engineered (TE) cartilage limits its clinical application. Focus has been on engineering cartilage with enhanced mechanical stiffness by reproducing native biochemical compositions. More recently, depth dependency of the biochemical content and the collagen network architecture has gained interest. However, it is unknown whether the mechanical performance of TE cartilage would benefit more from higher content of biochemical compositions or from achieving an appropriate collagen organization. Furthermore, the relative synthesis rate of collagen and proteoglycans during the TE process may affect implant performance. Such insights would assist tissue engineers to focus on those aspects that are most important. The aim of the present study is therefore to elucidate the relative importance of implant ground substance stiffness, collagen content, and collagen architecture of the implant, as well as the synthesis rate of the biochemical constituents for the post-implantation mechanical behavior of the implant. We approach this by computing the post-implantation mechanical conditions using a composition-based fibril-reinforced poro-viscoelastic swelling model of the medial tibia plateau. Results show that adverse implant composition and ultrastructure may lead to post-implantation excessive mechanical loads, with collagen orientation being

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Section: Discussionsupporting

confidence: 65%

The effect of tissue-engineered cartilage biomechanical and biochemical properties on its post-implantation mechanical behavior

Khoshgoftar

Wilson

Ito

et al. 2012

Biomech Model Mechanobiol

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show abstract

“…However, the integration of donor and recipient hyaline cartilage can be impaired because of diff erent mechanical properties and thickness. 79,147,241 Other possible disadvantages are the limited availability of graft s and the risk of donor site morbidity. 147,327 Furthermore, the use of a medial malleolar osteotomy to approach the lesion has been associated with a worse outcome, i.e., local osteoarthritis and higher morbidity 143 A randomized controlled trial with 2 years of follow-up comparing OATS with debridement (with or without microfracture) showed similar results among the methods.…”

Section: Discussionmentioning

confidence: 99%

Treatment of osteochondral defects of the talus

Bergen

Leeuw

Dijk

2008

Revue de Chirurgie Orthopédique et Réparatrice de l'Appareil Mo

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“…In fact, due to its nature of a higher-level vertebrate and non-pet status when compared to dogs, goats play a significant role in the orthopaedics field as a feasible model for orthotopic applications. In addition, goats not only have metabolic and bone remodelling rates similar to that of humans but also a comparable sequence of events in bone graft incorporation and healing capacities (Pearce et al, 2007), which explains the fact that this model has been frequently used in studies of bone formation and regeneration (Kruyt et al, 2004;Li et al, 2006;Zhu et al, 2006), biocompatibility (Mendes et al, 2001) and osteochondral regeneration (Lane et al, 2004;Niederauer et al, 2000).…”

Section: Introductionmentioning

confidence: 95%

Tissue-engineered constructs based on SPCL scaffolds cultured with goat marrow cells: functionality in femoral defects

Rodrigues

Gomes

Viegas

et al. 2010

J Tissue Eng Regen Med

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This study aims to assess the in vivo performance of cell-scaffold constructs composed of goat marrow stromal cells (GBMCs) and SPCL (a blend of starch with polycaprolactone) fibre mesh scaffolds at different stages of development, using an autologous model. GBMCs from iliac crests were seeded onto SPCL scaffolds and in vitro cultured for 1 and 7 days in osteogenic medium. After 1 and 7 days, the constructs were characterized for proliferation and initial osteoblastic expression by alkaline phosphatase (ALP) activity. Scanning electron microscopy analysis was performed to investigate cellular morphology and adhesion to SPCL scaffolds. Non-critical defects (diameter 6 mm, depth 3 mm) were drilled in the posterior femurs of four adult goats from which bone marrow and serum had been collected previously. Drill defects alone and defects filled with scaffolds without cells were used as controls. After implantation, intravital fluorescence markers, xylenol orange, calcein green and tetracycline, were injected subcutaneously after 2, 4 and 6 weeks, respectively, for bone formation and mineralization monitoring. Subsequently, samples were stained with Lévai-Laczkó for bone formation and histomorphometric analysis. GBMCs adhered and proliferated on SPCL scaffolds and an initial differentiation into pre-osteoblasts was detected by an increasing level of ALP activity with the culture time. In vivo experiments indicated that bone neoformation occurred in all femoral defects. The results obtained provided important information about the performance of SPCL-GBMC constructs in an orthotopic goat model that enabled future studies to be designed to investigate in vivo the functionality of SPCL-GBMC constructs in more complex models, viz. critical sized defects, and to evaluate the influence of in vitro cultured autologous cells in the healing and bone regenerative process.

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Follow-Up of Osteochondral Plug Transfers in a Goat Model

Abstract: Autogenous transplantation of osteochondral plugs is possible with integration of subchondral bone and preservation of chondral viability.

Cited by 128 publications

References 35 publications

The effect of tissue-engineered cartilage biomechanical and biochemical properties on its post-implantation mechanical behavior

The effect of tissue-engineered cartilage biomechanical and biochemical properties on its post-implantation mechanical behavior

Treatment of osteochondral defects of the talus

Tissue-engineered constructs based on SPCL scaffolds cultured with goat marrow cells: functionality in femoral defects

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