Follow up of patients with ocular scarring secondary to LOC syndrome treated by amniotic membrane transplantation

Moore, Johnny; Shah, Sunil; Kumar, Vinod; Ainsworth, JR; Page, AB; McLean, W.H. Irwin

doi:10.1136/bjo.2004.059121

Cited by 18 publications

(7 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, our own results showed a refractive surprise >1 D of 5% in the combined cataract and microbypass–stent group, which is comparable to both the nonglaucoma cataract-only group in Manoharan et al’s study and our own nonglaucoma cataract-only group. Additionally, other reports have demonstrated the negative impact of more invasive glaucoma surgeries on astigmatism 20–22…”

Section: Discussionmentioning

confidence: 99%

Refractive outcomes after trabecular microbypass stent with cataract extraction in open-angle glaucoma

Scott

Ferguson

Stephens

et al. 2019

OPTH

View full text Add to dashboard Cite

Purpose To evaluate the impact of trabecular microbypass stents combined with cataract surgery on refractive outcomes in patients with open-angle glaucoma (OAG). Setting Private practice, Sioux Falls, South Dakota, USA. Design Retrospective, comparative case series. Methods Eyes with OAG had implantation of trabecular microbypass stents with concomitant cataract surgery. The unmatched control group comprised eyes that underwent only cataract extraction. Data were collected preoperatively and postoperatively for 6 months. Data included spherical equivalent (SE), astigmatic error, intraocular pressure (IOP), and number of glaucoma medications. Results The series included 76 consecutive OAG eyes with combined cataract plus trabecular microbypass stent and 50 consecutive non-OAG eyes with cataract surgery only. SE outcomes were equivalent between the groups ( P <0.001). For the combined and cataract-only groups respectively, 46% vs 52% had SEs within 0.25 D of the target, 80% vs 80% within 0.50 D, and 95% vs 94% within 1.00 D. Astigmatism outcomes did not significantly differ between the groups ( P >0.05). As for magnitude of astigmatism in the combined and cataract only groups respectively, 51% vs 32% were within 0.5 D, 75% vs 66% within 1.0 D, 87% vs 82% within 1.5 D, and 89% vs 94% within 2.0 D. In the OAG combined–surgery group, mean intraocular pressure reduction was 3.4 mmHg ( P <0.0001) at 1 month postoperatively, 4.0 mmHg ( P <0.0001) at 3 months, and 3.4 mmHg ( P <0.01) at 6 months. Mean decrease in number of glaucoma medications was 0.4 ( P <0.05) at 1 month, 0.7 ( p <0.0001) at 3 months, and 0.9 ( P <0.001) at 6 months. Conclusion The results of this study suggest the trabecular microbypass stent is a refractively neutral device.

show abstract

Section: Discussionmentioning

confidence: 99%

Refractive outcomes after trabecular microbypass stent with cataract extraction in open-angle glaucoma

Scott

Ferguson

Stephens

et al. 2019

OPTH

View full text Add to dashboard Cite

show abstract

“…Fibronectin and a-SMA, the fibrotic markers, were decreased in keratocytes cultured on UAM compared with cell culture dish. Previous studies have proved full-thickness AM is effective in reducing scar formation during ocular surface reconstruction, 31 and soluble AM stromal extracts can prevent myofibroblast differentiation. 24 Our current results further proved that UAM retained the characteristic of antifibrosis on keratocytes during ex vivo tissue engineering, thus preserving their normal phenotype, which is critical in corneal stromal tissue engineering in terms of matrix synthesis.…”

Section: Discussionmentioning

confidence: 95%

A Novel Tissue-Engineered Corneal Stromal Equivalent Based on Amniotic Membrane and Keratocytes

Che

Jia

et al. 2019

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. To investigate a novel strategy in constructing tissue-engineered corneal stromal equivalent based on amniotic membrane and keratocytes. METHODS. The ultrathin amniotic membrane (UAM) was laminated, with corneal stromal cells (CSCs) distributed between the space of the layered UAMs. Calcein AM staining was used to evaluate cellular viability, morphology, and arrangement. Immunostaining, qRT-PCR, and Western blot were performed to detect gene and protein expression in keratocytes. Optical coherence tomography visualized the cross sections and thickness of the UAM construction. The microstructure of the CSC-secreted extracellular matrix (ECM) was investigated by scanning electron microscopy and transmission electron microscopy (TEM). To evaluate the feasibility of the multilayer UAM-CSC lamination for surgery, the corneal substitute was used to perform lamellar keratoplasty. Slit lamp microscopy and corneal fluorescein staining were performed in postsurgery observation. RESULTS. The CSCs maintained their keratocyte phenotype and secreted well-organized ECM on the aligned UAM surface. The four-layer UAM-CSC lamination attained half thickness of the human cornea (250 6 18 lm) after 8 weeks' culture, which also showed promising optimal transparency. In TEM images, the CSC-generated ECM displayed stratified, multilayered lamellae with orthogonal fibril arrangement, which was similar to the human cornea microstructure. Furthermore, the stromal equivalent was successfully preformed in lamellar keratoplasty. Four weeks post surgery, the substitute was well integrated into the recipient cornea and completely epithelialized without myofibroblast differentiation. CONCLUSIONS. Our study established a novel 3D biomimetic corneal model to replicate the corneal stromal organization with multilayer UAM, which was capable of promoting the development of corneal stroma-like tissues in vitro, establishing a new avenue for basic research and therapeutic potential.

show abstract

“…10 Treatment with a weekly office, 50% TTO eyelid scrub in addition to a daily 10% TTO shampoo scrub using a cotton tip was shown to be effective in reducing ocular signs and symptoms of Demodex blepharitis, conjunctivitis, and keratitis. [11][12][13][14] However, contact dermatitis, allergic reactions, and ocular irritation are well-known complications of TTO treatment 14 as a reaction to organic compounds, including terpinolene, αterpinene, ascaridol, and 1,2,4-trihydroxymethane. 15 Thus, elimination of these unwanted chemical ingredients in TTO should reduce ocular irritation and promote overall safety.…”

Section: Introductionmentioning

confidence: 99%

Improvement in ocular symptoms and signs in patients with Demodex anterior blepharitis using a novel terpinen-4-ol (2.5%) and hyaluronic acid (0.2%) cleansing wipe

Messaoud¹,

Fekih²,

Mahmoud³

et al. 2019

OPTH

View full text Add to dashboard Cite

Purpose: To evaluate the clinical improvement in ocular symptoms and signs in patients suffering from Demodex anterior blepharitis after using a novel cleansing wipe impregnated with 2.5% terpinen-4-ol and 0.2% hyaluronic acid. Study design: This was an exploratory, multicenter, open, randomized, two-parallel group comparative study. Methods: Forty-eight patients with Demodex anterior blepharitis were randomly assigned to apply the sterile wipe T1172 (Blephademodex ®), either once daily (n=24, Group 1) or twice daily (n=24, Group 2) for 29 days. Overall ocular discomfort and other individual symptoms were measured using a 0-10 numeric rating scale at Day 8 and Day 29. Ocular signs, including eyelid margin hyperemia and number of cylindrical dandruff, were examined at slit lamp. Overall treatment performance by investigator, patient satisfaction and tolerance were evaluated with questionnaires. Results: Overall ocular discomfort was significantly reduced from baseline (p<0.0001) in both groups at Day 8 (−3.6±0.3 in Group 1 and −4.0±0.4 in Group 2) and Day 29 (−5.7±0.4 and −6.8±0.7, respectively), with no difference between groups (D8: p=0.718; D29: p=0.505). Each ocular symptom associated with Demodex blepharitis was improved in both groups. Eyelid margin hyperemia was significantly reduced at Day 8 (−0.7±0.7; p<0.001) and Day 29 (−1.1±0.7; p<0.0001) in Group 1. Similar results were observed in Group 2 (Day 8: p<0.001; Day 29: p<0.0001). Total disappearance of cylindrical dandruff was reported in 30.4% of patients in Group 1 and 43.5% in Group 2. Improvements in other ocular signs were observed in both groups. The product was well tolerated. All patients were satisfied or very satisfied and would continue using it. Conclusion: Daily eyelid hygiene using this cleansing wipe impregnated with 2.5% terpinen-4-ol and 0.2% hyaluronic acid during a 4-week period led to a rapid and marked reduction in ocular symptoms and signs associated with Demodex anterior blepharitis and was well tolerated.

show abstract

Follow up of patients with ocular scarring secondary to LOC syndrome treated by amniotic membrane transplantation

Cited by 18 publications

References 16 publications

<p>Refractive outcomes after trabecular microbypass stent with cataract extraction in open-angle glaucoma</p>

<p>Refractive outcomes after trabecular microbypass stent with cataract extraction in open-angle glaucoma</p>

A Novel Tissue-Engineered Corneal Stromal Equivalent Based on Amniotic Membrane and Keratocytes

<p>Improvement in ocular symptoms and signs in patients with <em>Demodex</em> anterior blepharitis using a novel terpinen-4-ol (2.5%) and hyaluronic acid (0.2%) cleansing wipe</p>

Contact Info

Product

Resources

About