Follow-up of the genetic diversity and snail infectivity of a Schistosoma mansoni strain from field to laboratory

Bech, Nicolas; Beltran‐Bech, Sophie; Portela, Julien; Rognon, Anne; Allienne, Jean‐François; Boissier, Jérôme; Thèron, André

doi:10.1016/j.meegid.2010.06.012

Cited by 18 publications

(27 citation statements)

References 42 publications

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“…7). The factors and mechanisms underlying these differences are not yet known, but may include historical epidemiological conditions, differential selective pressures in the transmission areas, genotypic diversities in the host and parasitic isolates, recognition mechanisms developed through the matching-phenotypes model, and intraspecific competition among sporocysts (for details, see (Théron et al, 1997;Théron and Coustau, 2005;Théron et al, 2008;Bech et al). Notably, the host-parasite combination characterized by the higher infection rates and parasite intensities (SmBRE/BgBRE) was also characterized by a higher ROS-production capacity by the host and a higher ROS-scavenging ability by the parasite.…”

Section: Discussionmentioning

confidence: 99%

An example of molecular co-evolution: Reactive oxygen species (ROS) and ROS scavenger levels in Schistosoma mansoni/Biomphalaria glabrata interactions

Moné

Ribou

Cosseau

et al. 2011

International Journal for Parasitology

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Section: Discussionmentioning

confidence: 99%

An example of molecular co-evolution: Reactive oxygen species (ROS) and ROS scavenger levels in Schistosoma mansoni/Biomphalaria glabrata interactions

Moné

Ribou

Cosseau

et al. 2011

International Journal for Parasitology

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“…B. glabrata and S. mansoni originated from Brazil and have been maintained in the laboratory for several years [59]. The parasite strain was maintained in hamsters ( Mesocricetus auratus ), as described previously [60].…”

Section: Methodsmentioning

confidence: 99%

Biomphalysin, a New β Pore-forming Toxin Involved in Biomphalaria glabrata Immune Defense against Schistosoma mansoni

et al. 2013

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Aerolysins are virulence factors belonging to the β pore-forming toxin (β-PFT) superfamily that are abundantly distributed in bacteria. More rarely, β-PFTs have been described in eukaryotic organisms. Recently, we identified a putative cytolytic protein in the snail, Biomphalaria glabrata, whose primary structural features suggest that it could belong to this β-PFT superfamily. In the present paper, we report the molecular cloning and functional characterization of this protein, which we call Biomphalysin, and demonstrate that it is indeed a new eukaryotic β-PFT. We show that, despite weak sequence similarities with aerolysins, Biomphalysin shares a common architecture with proteins belonging to this superfamily. A phylogenetic approach revealed that the gene encoding Biomphalysin could have resulted from horizontal transfer. Its expression is restricted to immune-competent cells and is not induced by parasite challenge. Recombinant Biomphalysin showed hemolytic activity that was greatly enhanced by the plasma compartment of B. glabrata. We further demonstrated that Biomphalysin with plasma is highly toxic toward Schistosoma mansoni sporocysts. Using in vitro binding assays in conjunction with Western blot and immunocytochemistry analyses, we also showed that Biomphalysin binds to parasite membranes. Finally, we showed that, in contrast to what has been reported for most other members of the family, lytic activity of Biomphalysin is not dependent on proteolytic processing. These results provide the first functional description of a mollusk immune effector protein involved in killing S. mansoni.

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“…Consequently they are poorly representative of the genetic variation present in their original populations. A limited initial sampling of the diversity present in the field, laboratory host-induced selection, founder effects and bottlenecking processes during lab maintenance have been demonstrated to sharply reduce genetic diversity in laboratory strains, particularly for the parasite (Bech et al, 2010; Stohler et al, 2004). …”

Section: From Field Observations To a Theoretical Frameworkmentioning

confidence: 99%

“…2). This compatibility level remained relatively stable during the entire time the parasite strain was maintained (from 2005 to present), while the neutral genetic diversity of the schistosome strain declined steadily (Bech et al, 2010). …”

Section: From Field Observations To a Theoretical Frameworkmentioning

confidence: 99%

Compatibility polymorphism in snail/schistosome interactions: From field to theory to molecular mechanisms

Mitta

Adema

Gourbal

et al. 2012

Developmental & Comparative Immunology

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Coevolutionary dynamics in host–parasite interactions potentially lead to an arms race that results in compatibility polymorphism. The mechanisms underlying compatibility have remained largely unknown in the interactions between the snail Biomphalaria glabrata and Schistosoma mansoni, one of the agents of human schistosomiasis. This review presents a combination of data obtained from field and laboratory studies arguing in favor of a matching phenotype model to explain compatibility polymorphism. Investigations focused on the molecular determinants of compatibility have revealed two repertoires of polymorphic and/or diversified molecules that have been shown to interact: the parasite antigens S. mansoni polymorphic mucins and the B. glabrata fibrinogen-related proteins immune receptors. We hypothesize their interactions define the compatible/incompatible status of a specific snail/schistosome combination. This line of thought suggests concrete approaches amenable to testing in field-oriented studies attempting to control schistosomiasis by disrupting schistosome–snail compatibility.

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Follow-up of the genetic diversity and snail infectivity of a Schistosoma mansoni strain from field to laboratory

Cited by 18 publications

References 42 publications

An example of molecular co-evolution: Reactive oxygen species (ROS) and ROS scavenger levels in Schistosoma mansoni/Biomphalaria glabrata interactions

An example of molecular co-evolution: Reactive oxygen species (ROS) and ROS scavenger levels in Schistosoma mansoni/Biomphalaria glabrata interactions

Biomphalysin, a New β Pore-forming Toxin Involved in Biomphalaria glabrata Immune Defense against Schistosoma mansoni

Compatibility polymorphism in snail/schistosome interactions: From field to theory to molecular mechanisms

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