Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy

Darrier, Benoît; Arrieta, Mikel; Mittmann, Sybille U; Sourdille, Pierre; Ramsay, Luke; Waugh, Robbie; Colas, Isabelle

doi:10.1007/978-1-4939-9818-0_15

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Since it is not possible to find all the different stages in only one plant, the experiments were conducted over several weeks. In wheat, each floret corresponds to a different meiotic stage along the spike, but all three anthers per floret were synchronized [92]. For each line, we selected anthers of the same size, and we observed them to evaluate the meiotic stage before proceeding to the next steps.…”

Section: Immunostainingmentioning

confidence: 99%

Location and Identification on Chromosome 3B of Bread Wheat of Genes Affecting Chiasma Number

Darrier

Colas

Rimbert

et al. 2022

Plants

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Understanding meiotic crossover (CO) variation in crops like bread wheat (Triticum aestivum L.) is necessary as COs are essential to create new, original and powerful combinations of genes for traits of agronomical interest. We cytogenetically characterized a set of wheat aneuploid lines missing part or all of chromosome 3B to identify the most influential regions for chiasma formation located on this chromosome. We showed that deletion of the short arm did not change the total number of chiasmata genome-wide, whereas this latter was reduced by ~35% while deleting the long arm. Contrary to what was hypothesized in a previous study, deletion of the long arm does not disturb the initiation of the synaptonemal complex (SC) in early meiotic stages. However, progression of the SC is abnormal, and we never observed its completion when the long arm is deleted. By studying six different deletion lines (missing different parts of the long arm), we revealed that at least two genes located in both the proximal (C-3BL2-0.22) and distal (3BL7-0.63-1.00) deletion bins are involved in the control of chiasmata, each deletion reducing the number of chiasmata by ~15%. We combined sequence analyses of deletion bins with RNA-Seq data derived from meiotic tissues and identified a set of genes for which at least the homoeologous copy on chromosome 3B is expressed and which are involved in DNA processing. Among these genes, eight (CAP-E1/E2, DUO1, MLH1, MPK4, MUS81, RTEL1, SYN4, ZIP4) are known to be involved in the recombination pathway.

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Section: Immunostainingmentioning

confidence: 99%

Location and Identification on Chromosome 3B of Bread Wheat of Genes Affecting Chiasma Number

Darrier

Colas

Rimbert

et al. 2022

Plants

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“…The central element ZYP1 of the SC starts polymerizing during zygotene to form a protein complex resembling a zipper structure connecting the two meiotic homologous chromosomes from telomere to telomere at pachytene. The two AEs are called lateral elements once they form this tripartite structure ( Higgins et al, 2005 ; West et al, 2019 ; Darrier et al, 2020 ; Kurzbauer et al, 2021 ). Although two redundant ZYP1 proteins sharing 87% identity are present in Arabidopsis; their relative role is still unknown.…”

Section: Synapsis and Role Of The Sc In The Regulation Of Recombinationmentioning

confidence: 99%

The Formation of Bivalents and the Control of Plant Meiotic Recombination

et al. 2021

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During the first meiotic division, the segregation of homologous chromosomes depends on the physical association of the recombined homologous DNA molecules. The physical tension due to the sites of crossing-overs (COs) is essential for the meiotic spindle to segregate the connected homologous chromosomes to the opposite poles of the cell. This equilibrated partition of homologous chromosomes allows the first meiotic reductional division. Thus, the segregation of homologous chromosomes is dependent on their recombination. In this review, we will detail the recent advances in the knowledge of the mechanisms of recombination and bivalent formation in plants. In plants, the absence of meiotic checkpoints allows observation of subsequent meiotic events in absence of meiotic recombination or defective meiotic chromosomal axis formation such as univalent formation instead of bivalents. Recent discoveries, mainly made in Arabidopsis, rice, and maize, have highlighted the link between the machinery of double-strand break (DSB) formation and elements of the chromosomal axis. We will also discuss the implications of what we know about the mechanisms regulating the number and spacing of COs (obligate CO, CO homeostasis, and interference) in model and crop plants.

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Chromosome Synapsis and Recombination in Cereals

Orr

Lewandowska

Waugh

et al. 2021

Annual Plant Reviews Online

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Crops are under constant pressure due to climate change imposing dramatic changes in rainfall, temperature, and soil salinity. They are also under high stress due to pests and diseases, costing yield, and income loss to farmers. Thus, there is a need to create more resilient varieties. New favourable allelic combinations are achieved via homologous recombination during a process called meiosis. During meiosis, homologous chromosomes pair, synapse, and recombine; but crossover (CO) sites are rare, limiting the creation of valuable allelic recombinants. In large genome crops, such as barley or wheat, crossovers are not only scarce (2 or 3 per chromosome), they are also mainly located at the ends of the chromosomes, leaving 20–30% of genes in the pericentromeric region which breeding cannot exploit (https://youtu.be/XyMhyeWMZl4). The use of downregulated lines for specific meiotic gene candidates (e.g. RECQ4) has shown successful and promising translational research from the model plantArabidopsis thalianato crops, raising hopes for using this approach in breeding programs. The development of new tools, technologies, and protocols have demonstrated new insights into the progression of meiosis crops – highlighting unique meiotic phenomena not observed inArabidopsis– and revealed a higher cost of meiotic mutants on fertility than previously thought suggesting a more intricate relationship between meiotic events. In this article, we have selected a number of studies to show the relationship between synaptonemal complex formation and the recombination machinery.

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Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy

Abstract: Following the formation of synaptonemal complex formation in wheat and barley by high resolution microscopy.

Cited by 5 publications

References 16 publications

Location and Identification on Chromosome 3B of Bread Wheat of Genes Affecting Chiasma Number

Location and Identification on Chromosome 3B of Bread Wheat of Genes Affecting Chiasma Number

The Formation of Bivalents and the Control of Plant Meiotic Recombination

Chromosome Synapsis and Recombination in Cereals

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