Food allergens affect the intestinal tight junction permeability in inducing intestinal food allergy in rats

Chen, Tao; Liu, Xiaoyu; Ma, Li; He, Wenting; Li, Weizhong; Cao, Yanjuan; Liu, Zhigang

doi:10.12932/ap0443.32.4.2014

Cited by 26 publications

(24 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The significance of the similar findings in the pig system compared with our recent study in rat regarding claudin-4 and barrier properties (Markov et al, 2016 ) strongly suggests that a major common denominator has now been identified on both, protein and mRNA level. The pig system closely resembles the human intestine and therefore is a useful model for studying the detailed principles of PP FAE function and regulation in barriology and immunology, which are currently being discussed in the context of formula feeding and food allergies (Chen et al, 2014 ; Wu et al, 2015 ; Yeruva et al, 2016 ). However, as a refinement, current limitations regarding the visualization of co-localization and the quantification of immunohistological images might be overcome in epithelial monolayer and co-culture models.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Barrier Properties in Follicle-Associated Epithelium of Porcine Peyer's Patches Reveals Major Sealing Function of Claudin-4

et al. 2017

View full text Add to dashboard Cite

The pig represents a preferred model for the analysis of intestinal immunology. However, the barrier of the follicle-associated epithelium (FAE) covering porcine Peyer's patches (PP) has not yet been characterized in detail. This study aimed to perform this characterization in order to pave the way toward an understanding of the functional contribution of epithelial barrier properties in gut immunology. Porcine tissue specimens were taken from the distal small intestine in order to obtain electrophysiological data of PP FAE and neighboring villous epithelium (VE), employing the Ussing chamber technique. Transepithelial resistance (TER) and paracellular fluorescein flux were measured, and tissues were morphometrically compared. In selfsame tissues, expression and localization of major tight junction (TJ) proteins (claudin-1, -2, -3, -4, -5, and -8) were analyzed. PP FAE specimens showed a higher TER and a lower apparent permeability for sodium fluorescein than VE. Immunoblotting revealed an expression of all claudins within both epithelia, with markedly stronger expression of the sealing TJ protein claudin-4 in PP FAE compared with the neighboring VE. Immunohistochemistry confirmed the expression and localization of all claudins in both PP FAE and VE, with stronger claudin-4 abundance in PP FAE. The results are in accordance with the physiological function of the FAE, which strongly regulates and limits antigen uptake determining a mandatory transcellular route for antigen presentation, highlighting the importance of this structure for the first steps of the intestinal immune response. Thus, this study provides detailed insights into the specific barrier properties of the porcine FAE covering intestinal PP, at the interface of intestinal immunology and barriology.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Barrier Properties in Follicle-Associated Epithelium of Porcine Peyer's Patches Reveals Major Sealing Function of Claudin-4

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Juvenile rats exhibit a higher Ovalbumin sensitization rate compared to adult rats possibly by increasing permeability of intestinal tight junctions. [30] It is highly likely that the differences in food allergen sensitization between the adults and the children could be due to allergens related factors capable of interfering with tight junction permeability in the gut. Age-related intestinal barrier dysfunction observed in rats is characterized by mucosal atrophy, tight junction damage with decreased epithelial tight junction protein and increased intestine bacteria counts may also be an important factor in determining allergen sensitization among children and adults.…”

Section: Discussion:-mentioning

confidence: 99%

SCREENING FOR FOOD SPECIFIC IgE ANTIBODIES AMONG SAUDI PATIENTS WITH CLINICAL SUSPICION OF FOOD ALLERGY.

Shakoor¹,

Musibeeh²,

Najd³

et al. 2016

IJAR

View full text Add to dashboard Cite

Background/Aim: The prevalence of food allergy is increasing all over the world and varies in different geographical locations. This study was performed to assess allergic sensitization against various food materials among Saudi patients clinically presenting with food allergy. Patients and Methods:Data for the presence of food specific IgE antibodies were collected retrospectively from 280 Saudi patients screened between October 2012 and February 2014. These patients presented in the allergy clinic at King Khalid University Hospital, Riyadh with clinical signs and symptoms of food allergy.Out of the total 92 patients were found to have food specificIgE circulating antibodies. This group of patients comprised of 67 (72.8%) males and 25 (27.2%) females, with 78 (84.8%) children of <12 years and 14 (15.2%) adults (mean age 9.04±7.71 years). Food specific IgE antibodies were quantified by radioallergosorbent test (RAST) using Pharmacia ImmunoCAP 250 analyzer. Results: The most frequently sensitizing food allergens were milk in 57 (61.96%) patients followed by, egg white in 55 (59.78%) patients, wheat in 42 (45.65%) patients and peanut in 35 (38.04%) patients. Male children were consistently more sensitized against egg white (47.4%vs15.3%; p<0.0002), egg yolk (33.3%vs7.6%; p<0.0002), milk (47.4%vs22.1%; p<0.001), wheat (37.5%vs12.4%; p<0.0006) and peanut (37.5%vs11%; p<0.0007) compared to females. Milk sensitization was high among children whereas sensitization due to egg white was high in adults. Conclusion:Patients were frequently sensitized against milk, egg white, wheat and peanut particularly the male children.

show abstract

“…(1) Remoção dos alimentos contendo os alérgenos alimentares de interesse, aqueles de difícil digestão e que contêm pesticidas, além dos aditivos alimentares, dentre esses os polissorbatos, emulsificantes (como CMC), metabissulfito de sódio e fosfato de sódio. Todos esses aditivos podem provocar alteração das tight junctions e levar ao aumento da permeabilidade intestinal, o que contribui para a quebra da tolerância de mucosa 32,[39][40][41][42] ;…”

Section: Como Induzimos Na Prática Clínica a Tolerância De Mucosa Parunclassified

“…Essa correção pode ser realizada através da utilização de complexo de enzimas e tinturas fitoterápicas que melhorem a secreção de HCl e conscientização dos pacientes quanto ao uso dos medicamentos da classe dos prazóis [44][45][46] . Importante atentar para a possibilidade de super crescimento bacteriano ou fúngico, que também deverá ser corrigida para melhora do processo alérgico 42,47 ;…”

Section: Como Induzimos Na Prática Clínica a Tolerância De Mucosa Parunclassified

Tolerância de Mucosa: nova ou velha abordagem no tratamento das sensibilidades alimentares?

Campos¹

2018

Rev Bras Nutr Func

View full text Add to dashboard Cite

From a historical point of view, tolerance was first described in mid-1911, long before the description of its mechanisms. Today it is characterized by local hyperactivity with proliferation of regulatory T and B cells, production of anti-inflammatory cytokines, secretory IgA and stimulation to the microbiota for the production of short chain fatty acids, concomitant with systemic hyporesponsiveness with low IgG and IgE antibodies production. Perhaps due to these characteristics, the induction of immunological tolerance and in particular the mucosal tolerance (oral) has attracted considerable attention as a therapeutic possibility, especially for the treatment of food sensitivities. All the strategies used for intestinal modulation are also considered important for the recovery of mucosal tolerance and, to some extent, have been applied in practice, even though in an empirical or intuitive way, for many years.

show abstract

Food allergens affect the intestinal tight junction permeability in inducing intestinal food allergy in rats

Abstract: Our data suggested that the allergy sensitization rate of Ovalbumin (OVA) in the juvenile group is higher than in adults and food allergens may increase intestinal mucosal permeability through intestinal tight junction regulation in inducing intestinal food allergy.

Cited by 26 publications

References 34 publications

Molecular Characterization of Barrier Properties in Follicle-Associated Epithelium of Porcine Peyer's Patches Reveals Major Sealing Function of Claudin-4

Molecular Characterization of Barrier Properties in Follicle-Associated Epithelium of Porcine Peyer's Patches Reveals Major Sealing Function of Claudin-4

SCREENING FOR FOOD SPECIFIC IgE ANTIBODIES AMONG SAUDI PATIENTS WITH CLINICAL SUSPICION OF FOOD ALLERGY.

Tolerância de Mucosa: nova ou velha abordagem no tratamento das sensibilidades alimentares?

Contact Info

Product

Resources

About