Food-Borne Topoisomerase Inhibitors

Esselen, Melanie; Barth, Stefan

doi:10.1016/b978-0-444-63406-1.00004-0

Cited by 18 publications

(8 citation statements)

References 183 publications

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“…The weak signals of kaempferol, quercetin and myricetin were possibly because of the incompatible form between standards and flavonoids in nature. These flavonoids were usually in the glycoside or uronic acid conjugate form in the nature ( Bouderias et al, 2020 ; DuPont et al, 2004 ; Esselen and Barth, 2014 ; Taheri et al, 2020 ; Wang et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction

Chien

Saputri

Lin

2022

Current Research in Food Science

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As the highest yield crop worldwide, citrus peels that possess bioactive compounds were discarded as a futile by-product. Ultrasonication with environmentally friendly solvent (50% ethanol and ddH 2 O) were used in the present study to extract flavonoids from Citrus depressa Hayata peels with extraction period and fruit maturity as other variables. DPPH scavenging activity was investigated. Qualitative flavonoid content analysis was done by UV/Vis and FTIR-ATR spectra. Quantification of flavonoid using LC-MS/MS found that solvent type, fruit maturity, and ultrasonication period significantly affect the extracted flavonoid yield (p < 0.05). Extraction using 50% ethanol showed a higher yield than ddH 2 O. Flavonoid content was also higher in unripe than ripe samples. Nobiletin, tangeretin, and rutin were dominant among the identified compounds in all sample treatments. Flavonoid content in Citrus depressa Hayata extract was found to negatively correlate to DPPH scavenging activity, which needs further research to identify other bioactivities of these flavonoids.

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Section: Resultsmentioning

confidence: 99%

Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction

Chien

Saputri

Lin

2022

Current Research in Food Science

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“…Fisetin and myricetin reportedly behaved as dual inhibitors of topoisomerase I and II [48]. Some flavonols, quercetin, kaempferol, fisetin, and myricetin were reported to inhibit topoisomerase II with IC50 values of 19.9, 28.0, 28.0, 34.6 µM respectively [49]. Moreover, topoisomerase I bioassay guided isolation studies on Paphiopedilum callosum (Rchb.f.)…”

Section: Topoisomerase I and Ii Inhibition Assaymentioning

confidence: 99%

In vitro and in silico assessment of DNA interaction, topoisomerase I and II inhibition properties of chrysosplenetin

Şöhretoğlu

Barut

Sarı

et al. 2020

International Journal of Biological Macromolecules

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Chrysosplenetin is a methoxyflavone with reported anti-cancer effect. We tested its cytotoxic effect on the MCF-7 breast cancer cell line, and determined its effect on DNA intercalation and on the activity of topoisomerases I and II. The compound inhibited proliferation MCF-7 with an IC50 value of 0.29 µM. Chrysosplenetin did not initiate plasmid DNA cleavage but, in a concentrationdependent manner, protected plasmid DNA against damage induced by Fenton reagents. Furthermore, it possessed dual Topoisomerase I and II inhibitory properties. Especially, it inhibited topoisomerase II by 83-96% between the range 12.5-100 µM. In the light of these experimental findings, molecular docking studies were performed to understand binding mode, interactions and affinity of chrysosplenetin with DNA, and with topoisomerases I and II. These studies showed that of 4-chromone core and the hydroxyl and methoxy groups important for both intercalation with DNA and topoisomerase I and II inhibition.

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“…Its antioxidant, anti‐inflammatory, antidiabetic, anticarcinogenic, and antiviral activities have been well documented (Dormán et al., 2016; Leopoldini et al., 2011; Shukla et al., 2019). Studies have also demonstrated its structural–activity inhibitory role on topoisomerase activity through a link between DNA intercalation and topoisomerase structure (Esselen & Barth, 2014). Like many therapeutic flavonoids, MYC is a constituent of several medications including the Chinese herbal bayberry (Guo & Zheng, 2014).…”

Section: Introductionmentioning

confidence: 99%

An insight on the nature of biochemical interactions between glycyrrhizin, myricetin and CYP3A4 isoform

Sabiu

Idowu

2021

Journal of Food Biochemistry

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Drug interaction studies are imperative to gain insights into the beneficial or harmful effects of therapeutic and dietary agents. This study investigated the mechanism of modulatory roles of glycyrrhizin (GLH) and myricetin (MYC) on the human CYP3A4 isoform using in silico and in vitro methods. While MYC had concentration‐dependent inhibitory effect on CYP3A4 (IC50: 10.5 ± 0.55 μM) with characteristic Km and Vmax values of 1.13 μM and 1.54 nM/min, respectively, GLH exhibited no inhibitory effect on CYP3A4 activity in vitro. These observations are consistent with the results of in silico evaluations where the effect of MYC compared well with that of ketoconazole (a known CYP3A4 inhibitor) against CYP3A4. Overall, the established interactions between the study compounds and CYP3A4 could potentiate clinically vital drug–drug interactions and has lent credence to the mechanism of modulatory effect of MYC and GLH on CYP3A4 that could guide their safe use as therapeutic agents. Practical implications Myricetin (MYR) and glycyrrhizin (GLH) occur freely in commonly ingested foods and their supplements are recommended for the treatment of several debilitating diseases such as diabetes, cancer, and cardiovascular complications. This study provided an insight on the possible interactions that could be established between these compounds (MYR and GLH) and CYP3A4 when ingested and metabolized by the liver. The results suggested possibilities of potential clinical drug–drug interactions and advocates for their cautious use within the therapeutic dose in food supplements or medications to avoid probable liver damage.

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Food-Borne Topoisomerase Inhibitors

Cited by 18 publications

References 183 publications

Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction

Valorization of Taiwan's Citrus depressa Hayata peels as a source of nobiletin and tangeretin using simple ultrasonic-assisted extraction

In vitro and in silico assessment of DNA interaction, topoisomerase I and II inhibition properties of chrysosplenetin

An insight on the nature of biochemical interactions between glycyrrhizin, myricetin and CYP3A4 isoform

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