Food color induced hepatotoxicity in Swiss albino rats, Rattus norvegicus

Abstract

Objective:Certain dietary constituents can induce toxicity and play a critical role in the development of several hepatic disorders. Tartrazine, metanil yellow and sunset yellow are widely used azo dyes in food products, so the present study is aimed to investigate the food color induced hepatotoxicity in Swiss albino rats.Materials and Methods:Swiss albino rats were divided into four groups, each group having six animals. Group I served as control, Group II, Group III and Group IV were administered with 25, 50 and 75 mg/kg body weight blend of sunset yellow, metanil yellow and tartrazine for 30 days. Hepatotoxicity in rats treated with a blend of these food colors was studied by assessing parameters such as serum total protein, serum albumin, serum alkaline phosphatase (ALP) as well as hepatic malondialdehyde (MDA). The activity of superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT) were assessed.Results:Significantly increased concentrations of serum total protein, serum albumin, serum ALP and hepatic MDA and significantly lowered levels of SOD, reduced GSH and CAT in the liver tissue of treated animals were observed when compared with control animals. The alteration in the liver includes necrosis of hepatocytes, infiltration and vacuolation.Conclusion:The result indicates that consumption of food color in diet induces liver tissue damage. The used doses of food color were mostly attributable to hepatocellular damage and drastic alteration in antioxidant defense system.

Toxicological and safety assessment of tartrazine as a synthetic food additive on health biomarkers: A review

“…While the low dose of both tartrazine (15 mg/kg BW) and carmoisine (8 mg/kg BW) displayed a significant rise in the ALT and alkaline phosphatase activities, respectively as compared to the control rats (Amin et al, 2010). In addition, Saxena and Sharma (2015) reported that consumption of food color including tartrazine induces hepatic tissue damages in Swiss Albino Rats. These effects assessed through significant increased serum total protein, albumin, ALP and hepatic MDA level and significant lowered levels of SOD, reduced GSH and CAT in the hepatic tissue.…”

“…In a recent study, tartrazine, a widely used synthetic azo dye, induced a sharp deficiency in the biomarkers of antioxidant (SOD, catalase and GSH) and a marked rise in MDA concentration in the brain cortex in comparison with the other groups of male rat pups (Mohamed et al, 2015;Saxena and Sharma, 2015).…”

Peer Review #1 of "Tartrazine induces structural and functional aberrations and genotoxic effects in vivo (v0.1)"

“…In Manuscript to be reviewed who attributed similar changes in liver function to hepatocellular impairment. This liver damage would releases greater than normal levels of intracellular enzymes into the blood (Amin et al,2010;Himri et al, 2011;El-Wahab & Moram, 2015;Saxena &, Sharma, 2015).…”

Nephropathy Formation with Long-Term Using of Food Dyes Tartrazine (E102) in Experiment

“…Особливе місце серед актуа-льних проблем нефрології займає проблема ви-вчення факторів ризику розвитку та прогресування нефропатій. Визнано, що одна з вагомих екологіч-них причин високого рівня захворюваності нефро-патіями -це якість харчування [14].…”