2022
DOI: 10.38212/2224-6614.3393
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Food glycomics: Dealing with unexpected degradation of oligosaccharides during sample preparation and analysis

Abstract: This study reveals that unexpected degradation of food oligosaccharides can occur during conventional glycomics workflows, including sample preparation and analysis by liquid chromatography-mass spectrometry (LC-MS). With the present investigation, we aim to alert the scientific community of the susceptibility of specific glycosidic linkages to degradation induced by heat and acid. Key standard oligosaccharides representing the major types found in foods (3′-sialyllactose and 6′-sialyl-N-acetyllactosamine for … Show more

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Cited by 13 publications
(5 citation statements)
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“…Oligosaccharides were purified by a two-step SPE using C18 (HyperSep C18–96, 50 mg bed weight; Thermo Fisher Scientific) and PGC (HyperSep Hypercarb-96, 25 mg bed weight; Thermo Fisher Scientific) 56 . The samples were filtered (Captiva Premium Syringe Filter PES membrane, 4 mm diameter, 0.2 µm pore size, LC/MS certified) into 200 µl vials.…”
Section: Methodsmentioning
confidence: 99%
“…Oligosaccharides were purified by a two-step SPE using C18 (HyperSep C18–96, 50 mg bed weight; Thermo Fisher Scientific) and PGC (HyperSep Hypercarb-96, 25 mg bed weight; Thermo Fisher Scientific) 56 . The samples were filtered (Captiva Premium Syringe Filter PES membrane, 4 mm diameter, 0.2 µm pore size, LC/MS certified) into 200 µl vials.…”
Section: Methodsmentioning
confidence: 99%
“…Oligosaccharides are straight chain or branching carbohydrates consisting mainly of 3 to 20 sugar units [185]. Among them, xylo-oligosaccharide and inulin are often used as WRAs.…”
Section: Oligosaccharides and Their Derivativesmentioning
confidence: 99%
“…Oligosaccharides were purified by a two-step solid-phase extraction (SPE) using C18 (HyperSep C18-96, 50 mg bed weight; Thermo Fisher Scientific) and porous graphitic carbon (PGC) (HyperSep Hypercarb-96, 25 mg bed weight; Thermo Fisher Scientific) 55 . The samples were filtered (Captiva Premium Syringe Filter, polyethersulfone (PES) membrane, 4 mm diameter, 0.2 µm pore size, LC/MS certified) into 200 µl vials.…”
Section: Characterization Of Hmos By Lc-qtof-msmentioning
confidence: 99%