Food-Grade Organisms as Vaccine Biofactories and Oral Delivery Vehicles

Rosales‐Mendoza, Sergio; Angulo, Carlos; Meza, Beatriz

doi:10.1016/j.tibtech.2015.11.007

Cited by 97 publications

(62 citation statements)

References 96 publications

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“…The use of non-pathogenic bacteria, such as lactic acid bacteria (LAB), as a DNA delivery vehicle to the mucosal surface, could be a better and safer alternative for this purpose (Wells and Mercenier, 2008; Rosales-Mendoza et al, 2016). LAB, especially lactobacilli, and Lactococcus lactis , are used in the food industry and are classified as “ G enerally R egarded A s S afe” (GRAS) organisms due to their lack of pathogenicity, plus they have the criteria of the competent Q ualified P resumption of S afety (QPS) according to the European Food Safety Authority (EFSA).…”

Section: Introductionmentioning

confidence: 99%

Recombinant Invasive Lactococcus lactis Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-γ, IL-6, and TNF-α Cytokines after Intranasal Immunization

et al. 2017

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Tuberculosis (TB) remains a major threat throughout the world and in 2015 it caused the death of 1.4 million people. The Bacillus Calmette-Guérin is the only existing vaccine against this ancient disease; however, it does not provide complete protection in adults. New vaccines against TB are eminently a global priority. The use of bacteria as vehicles for delivery of vaccine plasmids is a promising vaccination strategy. In this study, we evaluated the use of, an engineered invasive Lactococcus lactis (expressing Fibronectin-Binding Protein A from Staphylococcus aureus) for the delivery of DNA plasmid to host cells, especially to the mucosal site as a new DNA vaccine against tuberculosis. One of the major antigens documented that offers protective responses against Mycobacterium tuberculosis is the Ag85A. L. lactis FnBPA+ (pValac:Ag85A) which was obtained and used for intranasal immunization of C57BL/6 mice and the immune response profile was evaluated. In this study we observed that this strain was able to produce significant increases in the amount of pro-inflammatory cytokines (IFN-γ, TNF-α, and IL-6) in the stimulated spleen cell supernatants, showing a systemic T helper 1 (Th1) cell response. Antibody production (IgG and sIgA anti-Ag85A) was also significantly increased in bronchoalveolar lavage, as well as in the serum of mice. In summary, these findings open new perspectives in the area of mucosal DNA vaccine, against specific pathogens using a Lactic Acid Bacteria such as L. lactis.

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Section: Introductionmentioning

confidence: 99%

Recombinant Invasive Lactococcus lactis Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-γ, IL-6, and TNF-α Cytokines after Intranasal Immunization

et al. 2017

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“…However, they optimized the expression of EspB in L. lactis through secretion of EspB either under constitutive or nisin-inducible control, and the strains successfully induced immune responses (Ahmed et al, 2014). Collectively, we speculated that an oral vaccine would be more effective, than LAB vaccines that are engineered to co-express antigens with cytokines or made inducible in different mutants, or new strains that are selected to have enhanced adjuvant or targeting capacities (Rosales-Mendoza et al, 2016). In our study, the antigen expressed by LA-ET was not strong, which was shown by SDS-PAGE analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Vaccines employing a mucosal delivery system can elicit both antigen-specific secretory immunoglobulin A (sIgA) and effective systemic immune responses. The relatively low cost, ease of administration, and degree of mucosal tissue protection make LAB more advantageous as vaccine delivery vectors (Rosales-Mendoza et al, 2016). Lactococcus lactis is most often used for EHEC and other pathogen vaccines.…”

Section: Introductionmentioning

confidence: 99%

Oral Immunization with Recombinant Lactobacillus acidophilus Expressing espA-Tir-M Confers Protection against Enterohemorrhagic Escherichia coli O157:H7 Challenge in Mice

et al. 2017

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Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) causes hemorrhagic colitis and the formation of characteristic attaching and effacing (A/E) lesions in humans. Given the severe sequelae of EHEC O157:H7 infection, it is critical to develop effective vaccines for human use. However, for achieving this goal many hurdles need to be addressed, such as the type or subset of antigens, adjuvant, and the delivery route. We developed a candidate vaccine by inserting the bivalent antigen espA-Tir-M composed of espA and the Tir central domain into Lactobacillus acidophilus. The recombinant L. acidophilus (LA-ET) was safe in a cell model and excluded EHEC O157:H7 from LoVo cells at rates of nearly 94 and 60% in exclusion and competition assays, respectively. LA-ET inhibited the induction of A/E lesions by EHEC O157:H7 cells in vitro. Oral immunization with LA-ET induced higher levels of specific mucosal and systemic antibody responses in mice. Moreover, LA-ET enhanced interferon-γ and interleukin-4 and -10 production, which was associated with mixed helper T (Th1/Th2) cell responses, and protected against EHEC O157:H7 colonization and infection in mice at a rate of 80%. Histopathological analyses revealed that orally administered LA-ET reduced or inhibited A/E lesions and toxin-induced systemic injury. These findings demonstrate that LA-ET induces both humoral and cellular immune responses in mice and is therefore a promising vaccine against EHEC O157:H7 infection.

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“…First, as the biopharmaceutical industry showed, plant vaccines would probably be lower production costs and it could be easy to scale. The victory of the experimental Ebola drug ZMapp manufactured in Nicotiana plants led to the gain of visibility in molecular farming [60,61]. Unlike the production of biomolecule, however, the edible vaccine formulations require no pre-administration treatment or purification, which further lowers the cost involved with production.The most studies have utilized cultivated potatoes but, as cooking or boiling can weaken most of antigenic proteins, potatoes might not be the best choice in edible vaccines [62].…”

Section: Plant-based Edible Vaccinesmentioning

confidence: 99%

Edible Vaccines: Promises and Challenges

2019

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Vaccines are biological preparations that improve immunity to particular diseases and form an important innovation of 19th century research. It contains a protein that resembles a disease-causing microorganism and is often made from weak or killed forms of the microbe. Vaccines are agents that stimulate the body's immune system to recognize the antigen. Now, a new form of vaccine was introduced which will have the power to mask the risk side of conventional vaccines. This type of vaccine was produced from plants which are genetically modified. In the production of edible vaccines, the gene-encoding bacterial or viral disease-causing agent can be incorporated in plants without losing its immunogenic property. The main mechanism of action of edible vaccines is to activate the systemic and mucosal immunity responses against a foreign diseasecausing organism. Edible vaccines can be produced by incorporating transgene in to the selected plant cell. At present edible vaccine are developed for veterinary and human use. But the main challenge faced by edible vaccine is its acceptance by the population so that it is necessary to make aware the society about its use and benefits. When compared to other traditional vaccines, edible vaccines are cost effective, efficient and safe. It promises a better prevention option from diseases.

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Food-Grade Organisms as Vaccine Biofactories and Oral Delivery Vehicles

Cited by 97 publications

References 96 publications

Recombinant Invasive Lactococcus lactis Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-γ, IL-6, and TNF-α Cytokines after Intranasal Immunization

Recombinant Invasive Lactococcus lactis Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-γ, IL-6, and TNF-α Cytokines after Intranasal Immunization

Oral Immunization with Recombinant Lactobacillus acidophilus Expressing espA-Tir-M Confers Protection against Enterohemorrhagic Escherichia coli O157:H7 Challenge in Mice

Edible Vaccines: Promises and Challenges

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