“…1 Provided (kg of DM): 500,000 IU of vitamin A, 200,000 IU of vitamin D3, 1000 mg of vitamin E, 500 mg of antioxidants, 190 g of Ca, 80 g of P, 21 g of Mg, 3000 mg of Fe, 60 g of Na, 300 mg of Cu, 2000 mg of Mn, 3000 mg of Zn, 100 mg of Co, 100 mg of I, and 30 mg of Se 2 Rumen degradable protein 3 Rumen undegradable protein 4 Net energy for lactation were estimated using the NASEM 2021 equation [14] In this part of the experiment, it was tried to record the amount of gas production and DM degradation of the experimental treatments simultaneously. In this way, for each incubation time (2,4,6,12,24,36, and 48 h), 4 vials (replicates) were allocated to each of the experimental treatments, and 4 vials were assigned to the blank as well. Each run contains 224 vials (including 7 experimental treatments plus blank, 7 incubation times, and 4 replications).…”