Food peptidomics of in vitro gastrointestinal digestions of partially purified bovine hemoglobin: low‐resolution versus high‐resolution LC‐MS/MS analyses

Abstract

Consumers and governments have become aware how the daily diet may affect the human health. All proteins from both plant and animal origins are potential sources of a wide range of bioactive peptides and the large majority of those display health-promoting effects. In the meat production food chain, the slaughterhouse blood is an inevitable co-product and, today, the blood proteins remain underexploited despite their bioactive potentiality. Through a comparative food peptidomics approach we illustrate the impact of resolving power, accuracy, sensitivity, and acquisition speed of low-resolution (LR)- and high-resolution (HR)-LC-ESI-MS/MS on the obtained peptide mappings and discuss the limitations of MS-based peptidomics. From in vitro gastrointestinal digestions of partially purified bovine hemoglobin, we have established the peptide maps of each hemoglobin chain. LR technique (normal bore C18 LC-LR-ESI-MS/MS) allows us to identify without ambiguity 75 unique peptides while the HR approach (nano bore C18 LC-HR-ESI-MS/MS) unambiguously identify more than 950 unique peptides (post-translational modifications included). Herein, the food peptidomics approach using the most performant separation methods and mass spectrometers with high-resolution capabilities appears as a promising source of information to assess the health potentiality of proteins.

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…Several of the Foodomics works recently published have involved the use of other different strategies and have allowed the study of the effects of extracellular production of hydrogen peroxide by rosemary polyphenols on the anti‐proliferative activity of rosemary polyphenols against HT‐29 colon cancer cells or how these compounds, i.e., rosemary polyphenols, induce unfolded protein response and changes in cholesterol metabolism in HT29 colon cancer cells . These different analytical strategies have also involved the use of other liquid techniques (e.g., UHPLC, nano‐LC) hyphenated to high resolution mass spectrometry and they have allowed the investigation of the faecal metabolomic fingerprint after moderate consumption of red wine , the peptidomic study of the in vitro gastrointestinal digestion of bovine haemoglobin .…”

A Caenorhabditis elegans Mass Spectrometric Resource for Neuropeptidomics

“…For instance, a comparative experiment using a nanobore high-resolution C18-LC-ESI-MS/MS and a low-resolution standard bore C18-LC-ESI-MS/MS system revealed a twelvefold increase in unique peptide identifications from gastrointestinal digestions of hemoglobin using the high-resolution system [28].…”

Targeted hydrolysis of native potato protein: A novel route for obtaining hydrolysates with improved interfacial properties

“…In general, a lower number of peptides were identified in PPHs produced using Neut, although more than 10,000 peptides were identified in all PPHs (Table 4). This is a tremendous increase in depth of analysis compared to previous reports of LC-MS/MS analysis on hydrolysates, where the number of identified peptides is often reported in the tens to low thousands range (Caron et al, 2016;Cui, Sun, Cheng, & Guo, 2022;Hinnenkamp & Ismail, 2021;Y. P. Huang, Dias, Leite Nobrega de Moura Bell, & Barile, 2022;Jin, Yan, Yu, & Qi, 2015;M.…”