Food prospects of selenium enriched-Lactobacillus acidophilus CRL 636 and Lactobacillus reuteri CRL 1101

Pescuma, Micaela; Gomez-Gomez, Beatríz; Pérez-Corona, Teresa; Font, Graciela; Madrid, Yolanda; Mozzi, Fernanda

doi:10.1016/j.jff.2017.06.009

Cited by 49 publications

(39 citation statements)

References 26 publications

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“…The literature data presented by Kitijama et al [23] and Brozmanová et al [24] indicate that the inhibitory effect of selenium on yeast cells results from changes in the composition and potential occurring in the cell membranes. The results of the study presented by Pescuma [25] also confirmed the inhibitory effect of selenium on Lactobacillus growth. The lowest dose of selenium used (4 mg/L) reduced the number of cells to 5.5 × 10 8 cfu/mL as compared to the yeast cells grown in the control medium (YPD) after 24 h of cultivation.…”

Section: Effect Of Selenium On the Number Of Yeast Cellssupporting

confidence: 52%

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

et al. 2020

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Selenium is considered an essential component of all living organisms. Studies on the enrichment of yeast cells with selenium, using the ability of cell biomass to bind this element, are being reported more and more. Yeast cultures were cultivated in YPD medium enriched with Na2SeO3 salts for 72 h at 28 °C on a shaker utilizing reciprocating motion. Selenium in cell biomass was determined with the use of ICP–MS. It was observed that the addition of selenium to the experimental medium (in the range of 4–100 mg/L) increased the content of this element in the yeast cell biomass. During the extension of cultivation time, the number of yeast cells and biomass yield exhibited a decreasing trend. Based on the obtained results, it was concluded that yeast cells exhibited the ability to accumulate selenium in both logarithmic and stationary growth phases. The dose of 20 and 30 mg/L of selenium in the culture medium meets the expectations in terms of both the content of selenium bound to yeast cells (1944 ± 110.8 μg/g dry weight) under 48-h cultivation. The obtained results confirmed that the Candida utilis ATCC 9950 strain exhibits the ability to bind selenium, which means that the biomass of these yeasts may be used as a natural source of selenium in the diet of humans and animals.

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Section: Effect Of Selenium On the Number Of Yeast Cellssupporting

confidence: 52%

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

et al. 2020

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“…Cells were grown in MRS or MRSf at 30 • C for 24 h in the absence or presence of 5 ppm Se and then centrifuged at 4,600 × g for 5 min. For this purpose, supernatants and bacterial cell pellets (0.1 g obtained from 10 ml culture) were submitted to acid digestion in a 1000-W microwave oven (MSP microwave oven, CEM, Matthews, NC, USA) using closed vessels containing 1 ml of concentrated HNO 3 and 0.5 ml of 30% (v/v) H 2 O 2 (Pescuma et al, 2017). The resulting solutions were cooled, diluted to a 25-ml final volume with MilliQ water and analyzed for the Se concentration with an Agilent 7700-collision/reaction cell ICP-MS (Agilent Technologies, Santa Clara, CA, USA).…”

Section: Intracellular Se Accumulation As Determined By Inductively Cmentioning

confidence: 99%

“…Some LAB can accumulate and biotransform Se salts into seleno-amino acids and SeNPs (Moreno-Martin et al, 2017;Pescuma et al, 2017); however, the metabolic pathways by which LAB bio-convert selenite remain unclear. It has been established that selenite may react with glutathione producing selenotrisulfide derivatives, which are intermediates in the conversion of inorganic Se into bioactive selenocompounds.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, selenocysteine lyase (SCL) is able to decompose SeCys to Se 0 and to mobilize Se into SeCys for selenophosphate synthesis necessary for producing SeCys t RNA, the precursor of SeCys and selenoproteins (Lamberti et al, 2011). Both GR and SCL have been detected in some LAB (Lamberti et al, 2011;Pusztahelyi et al, 2015); however, Se metabolism has been studied mainly in dairy-or human gut-origin strains (Zhang et al, 2009;Mangiapane et al, 2014;Palomo et al, 2014;Saini et al, 2014;Deng et al, 2015;Pescuma et al, 2017;Gomez-Gomez et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Biotransformation of Selenium by Lactic Acid Bacteria: Formation of Seleno-Nanoparticles and Seleno-Amino Acids

Martínez

Moreno-Martín

Pescuma

et al. 2020

Front. Bioeng. Biotechnol.

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Selenium (Se) is an essential micronutrient for the majority of living organisms, and it has been identified as selenocysteine in the active site of several selenoproteins such as glutathione peroxidase, thioredoxin reductase, and deiodinases. Se deficiency in humans is associated with viral infections, thyroid dysfunction, different types of cancer, and aging. In several European countries as well as in Argentina, Se intake is below the recommended dietary Intake (RDI). Some lactic acid bacteria (LAB) can accumulate and bio-transform selenite (toxic) into Se-nanoparticles (SeNPs) and Se-amino acids (non-toxic). The microbial growth, Se metabolite distribution, and the glutathione reductase (involved in selenite reduction) activity of Se-enriched LAB were studied in this work. The ninety-six assayed strains, belonging to the genera Lactococcus, Weissella, Leuconostoc, Lactobacillus, Enterococcus, and Fructobacillus could grow in the presence of 5 ppm sodium selenite. From the total, eight strains could remove more than 80% of the added Se from the culture medium. These bacteria accumulated intracellularly between 1.2 and 2.5 ppm of the added Se, from which F. tropaeoli CRL 2034 contained the highest intracellular amount. These strains produced only the selenoamino acid SeCys as observed by LC-ICP-MS and confirmed by LC-ESI-MS/MS. The intracellular SeCys concentrations were between 0.015 and 0.880 ppm; Lb. brevis CRL 2051 (0.873 ppm), Lb. plantarum CRL 2030 (0.867 ppm), and F. tropaeoli CRL 2034 (0.625 ppm) were the strains that showed the highest concentrations. Glutathione reductase activity values were higher when the strains were grown in the presence of Se except for the F. tropaeoli CRL 2034 strain, which showed an opposite behavior. The cellular morphology of the strains was not affected by the presence of Se in the culture medium; interestingly, all the strains were able to form spherical SeNPs as determined by transmission electron microscopy (TEM). Only two Enterococcus strains produced the volatile Se compounds dimethyl-diselenide identified by GC-MS. Our results show that Lb. brevis CRL 2051, Lb. plantarum CRL 2030, and F. tropaeoli CRL 2034 could be used for the development of nutraceuticals or as starter cultures for the bio-enrichment of fermented fruit beverages with SeCys and SeNPs.

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“…Probiotics are living microorganisms which, by ingesting a certain amount, can produce a basic nutritional value in the body beyond their own and have a health‐care effect (Pescuma et al, 2017). The conversion of inorganic selenium into organic selenium by probiotics to obtain the selenium‐rich probiotics, which not only keeps the effect of organic selenium, but also possesses the function of probiotics (Shen et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Screening, identification, and application of selenium‐enriched Lactobacillus in goat milk powder and tablet

Shu

Mei

Chen

et al. 2020

J Food Process Preserv

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In this study, Lactobacillus L20 was screened by measuring the tolerance to the inorganic selenium and the resistance to the gastrointestinal fluid of 11 Lactobacillus preserved in the laboratory. Next, the selenium‐enriched L20 was produced into the corresponding selenium‐enriched L20 goat milk powder and milk tablet. The storage stability of the prepared powder and tablet were investigated by the accelerated test. The results indicated that the conversion rate of inorganic selenium of L20 was 87.65% and the viable count was 2.925 × 108 CFU/ml. The results of 16S rDNA homology of partial sequences of molecular characterization suggested that Lactobacillus L20 was Lactobacillus rhamnosus. More notably, the inactivation rate constants and the predicted shelf life of selenium‐enriched L. rhamnosus L20 goat milk powder and milk tablet were 2.40 × 10–4 and 1.90 × 10–4 day–1 and 285 and 406 days under 25°C, respectively, exhibiting a considerable long‐term stability. Practical applications Selenium is one of the necessary microelements in human metabolism. Lactic acid bacteria can transform the inorganic selenium into organic selenium and selenium‐enriched Lactic acid bacteria can supply organic selenium and play its probiotic function. In this study, Lactobacillus rhamnosus L20 with strong selenium‐enriching ability was obtained and freeze‐dried powder was prepared. Selenium‐enriched probiotic goat milk powder and goat milk tablets were developed. The shelf life was predicted and prepared for selenium‐enriched probiotic powder. This work also provides a technical support for the subsequent industrialization of Se‐enriched probiotic goat milk powder and tablets.

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Food prospects of selenium enriched-Lactobacillus acidophilus CRL 636 and Lactobacillus reuteri CRL 1101

Cited by 49 publications

References 26 publications

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

Biotransformation of Selenium by Lactic Acid Bacteria: Formation of Seleno-Nanoparticles and Seleno-Amino Acids

Screening, identification, and application of selenium‐enriched Lactobacillus in goat milk powder and tablet

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