Food Restriction Suppresses an Age-Dependent Increase in the Exhalation Rate of Pentane From Rats: A Longitudinal Study

Abstract

Longitudinal age changes in the exhalation rates of ethane and pentane, which are used as the indices of in vivo lipid peroxidation, from rats under ad libitum feeding and food restriction were measured. The exhalation rate of ethane or pentane from old, ad libitum-fed rats is higher than that of the corresponding hydrocarbon from young, ad libitum-fed rats, as reported previously. There is no difference between the exhalation rates of each hydrocarbon from young, ad libitum-fed and food-restricted rats. However, the exhalation rate of pentane from old, food-restricted rats is significantly lower than that from old, ad libitum-fed rats, while the exhalation rate of pentane from old, food-restricted rats tends to be lower than that from old, ad libitum-fed rats. In addition, the exhalation rate of pentane from old, food-restricted rats is equivalent to that from young, ad libitum-fed rats. These results are consistent with the views that in vivo lipid peroxidation in rats is increased during aging, and that the age-dependent increase in in vivo lipid peroxidation is suppressed by food restriction.

Oxidative Stress, Caloric Restriction, and Aging

“…Experimental results tend to support these predictions. Rodents subjected to CR show attenuation of ageassociated increases in rates of mitochondrial and H 2 O 2 generation (50), slower accrual of oxidative damage (50,51), decreased alkane production (52), and delayed loss of membrane fluidity (53). Activities of individual antioxidative enzymes in different tissues do not follow a consistent pattern during aging or in response to CR (19,20,50,54); however, CR decreases the in vitro susceptibility of tissues to acute oxidative stress (55).…”

Acetone Concentration in Gas Emanating from Tails of Diabetic Rats

“…Some researchers previously examined volatile hydrocarbons, such as ethane and pentane, with reference to lipid peroxidation in exhaled air and from the whole body in rats. [12][13][14][15] However, there are no reports referencing the volatile organic compounds from rat tails. The collection of skin gas from rat tail has advantages compared to that of skin gas from humans which has been previously reported.…”

“…12 In that experimental system, animals produced feces and intestinal gas, which themselves effect the concentrations of hydrocarbons. Although collection systems that can remove hydrocarbon contaminant (feces and intestinal) gas have been developed, 13 they are very complicated. [13][14][15] In this study, skin gas is easily collected from a rat tail, which can avoid hydrocarbon contaminants.…”

“…Although collection systems that can remove hydrocarbon contaminant (feces and intestinal) gas have been developed, 13 they are very complicated. [13][14][15] In this study, skin gas is easily collected from a rat tail, which can avoid hydrocarbon contaminants. The method we used in this study is the first collecting system for the skin gas acetone concentration from diabetic rats.…”

Teorias biológicas do envelhecimento: do genético ao estocástico

“…Uma vez que o metabolismo energético gera molé-culas reativas de oxigênio potencialmente danosas às estruturas celulares, um possível mecanismo bioquímico daí nasce. Desde então, muitos estudos demonstraram que menor ingestão calórica tende a atenuar o processo de dano celular à medida que se envelhece, com redução de peroxidação lipídica 57 , menor acúmulo de proteínas oxidadas 58 e danificação oxidativa do DNA 59 . O problema é que essa hipótese vincula o efeito 'protetor' da restrição calórica à menor taxa metabólica dela decorrente.…”