The effects of high oleic acid rapeseed oil compared with polyunsaturated fats on serum lipoprotein levels are largely unknown. Therefore, we fed 30 women and 29 men a baseline diet rich in saturated fat, which was followed by a diet rich in high oleic and low erucic acid rapeseed oil (total energy content of fat, 38%; saturates, 12.4%; monounsaturates, 16%; n-6 polyunsaturates, 6%; and n-3 polyunsaturates, 2%) and one rich in sunflower oil (total energy content of fat, 38%; saturates, 12.7%; monounsaturates, 10%; n-6 polyunsaturates, 13%; and n-3 polyunsaturates, 0%). The oils were incorporated into mixed natural diets that were dispensed in a random order for 3.5 weeks each in a blinded crossover design. The diet composition was confirmed by analysis of duplicate diets. Both test diets reduced serum total cholesterol (TC) and low density lipoprotein (LDL) cholesterol levels from baseline, the monounsaturated rapeseed oil diet more than the polyunsaturated sunflower oil diet (TC: -15% versus -12%, p<0.01; LDL cholesterol: -23% versus -17%, p<0.0l). Very low density lipoprotein (VLDL) cholesterol and total, VLDL, and LDL triglyceride levels were lower during the sunflower oil diet compared with the rapeseed oil diet Total high density lipoprotein (HDL) cholesterol levels remained unchanged by both diets. The consumption of rapeseed oil resulted in a more favorable HDLj to LDL cholesterol ratio (0.43±0.19 versus 0J9±0.18, /?<0.01) and an apolipoprotein A-I to B ratio (3.0±1.4 versus 2.4± 1.6,p<0.001) than did the sunflower oil. Our results suggest that substitution by high oleic acid rapeseed oil of saturated fats strongly affects LDL metabolism, whereas substitution by polyunsaturated sunflower oil affects both VLDL and in addition to LDL. (Arteriosclerosis and Thrombosis 1992;12:50-57)