Foodborne outbreak of gastroenteritis due to Norovirus and <i>Vibrio parahaemolyticus</i>

Sala, M.; Arias, César; Domı́nguez, Àngela; Bartolomé, Rosa; Muntada, J. M.

doi:10.1017/s0950268808001040

Cited by 38 publications

(22 citation statements)

References 14 publications

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“…Human gastrointestinal infections are most commonly associated with the consumption of raw, undercooked or contaminated fish and shellfish (Yeung and Boor 2004; Reen et al. 2006; Su and Liu 2007; Sala et al. 2009).…”

Section: Introductionmentioning

confidence: 99%

Proteomic identification of membrane proteins regulating antimicrobial peptide resistance inVibrio parahaemolyticus

Shen¹,

Kuo²,

Lin³

et al. 2010

Journal of Applied Microbiology

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Aims: To identify proteins regulating antimicrobial peptide (AMP) resistance in Vibrio parahaemolyticus using membrane subproteome analysis. Methods and Results: Three synthetic AMPs (Q4, Q6 and H1) and a natural one from fish (pleurocidin) were used for selection of AMP‐resistant strains. Differential expression patterns of the outer and inner membrane proteins (OMPs and IMPs) among wild‐type and the resistant strains were obtained using two‐dimensional gel electrophoresis. Two OMPs (TolC and flagellin) and five IMPs [transcription termination factor (NusA), long‐chain fatty acid transport protein (FadL), elongation factor Tu (EF‐Tu), ATP synthase F1, alpha subunit (F1‐ATPa) and dihydrolipoamide dehydrogenase (DLD)] were identified using LC‐ESI‐Q‐TOF MS/MS and Mascot program. Real‐time quantitative polymerase chain reaction was also performed to determine the mRNA expression level of the target genes. All seven membrane proteins except FadL were upregulated in the AMP‐resistant clones, both in the translational and transcriptional levels. Conclusions: Our results suggested that V. parahaemolyticus may obtain their resistance against AMPs through upregulation of the multidrug efflux transporter, effective repair of damaged membranes and prevention of cellular penetration of AMPs. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing bacterial AMP resistance mechanism using proteomic methodologies. Elucidating the mechanism could help in the development of more sustainable antimicrobial agents.

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Section: Introductionmentioning

confidence: 99%

Proteomic identification of membrane proteins regulating antimicrobial peptide resistance inVibrio parahaemolyticus

Shen¹,

Kuo²,

Lin³

et al. 2010

Journal of Applied Microbiology

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“…2006). Vibrio parahaemolyticus causes foodborne gastroenteritis that is most frequently associated with the consumption of raw, undercooked or contaminated seafood (Yeung and Boor 2004; Su and Liu 2007; Sala et al. 2009).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis reveals responsive proteins of Vibrio parahaemolyticus on exposure to cationic antimicrobial peptides

Chiu

Kuo

Lin

et al. 2010

Journal of Applied Microbiology

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Aims: To investigate whether Vibrio parahaemolyticus can sense and directly respond to the presence of cationic antimicrobial peptides (AMPs). Methods and Results: We performed proteomic methodologies to investigate the responsive proteins of V. parahaemolyticus on exposure to AMP Q6. Differential expression patterns of the outer membrane, inner membrane and cytoplasmic proteins (OMPs, IMPs and CPs) from the bacteria with or without Q6 treatment were obtained using two‐dimensional gel electrophoresis (2‐DE). Three OMPs (maltoporin, flagellin and OmpV), two IMPs (ATP synthase F1, alpha subunit; and OmpV) and three CPs (pyruvate dehydrogenase subunit E1, glyceraldehyde‐3‐phosphate dehydrogenase and inositol‐5‐monophosphate dehydrogenase) were identified using LC‐ESI‐Q‐TOF MS/MS and Mascot program. Real‐time quantitative polymerase chain reaction was also performed to determine the mRNA expression level of the target genes. Conclusions: Our results suggested that V. parahaemolyticus may directly respond to AMPs through the upregulation of the efflux channel, increased yield of energy, effective repair of damaged membranes and downregulation of carbohydrate and nucleotide metabolism for energy preservation. Significance and Impact of the Study: AMP‐responsive proteins identified in the current study could serve as attractive targets for developing more effective antimicrobial agents.

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“…However, to our knowledge, they have been described only in hospitals [5,6] and the community [7,8] in Spain. In Majorca, only one norovirus outbreak in a nursing home for the elderly has been reported [9].…”

mentioning

confidence: 99%

Gastroenteritis outbreak caused by norovirus associated with the children's club of a hotel located in Majorca, Spain

Doménech-Sánchez

2011

Clinical Microbiology and Infection

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A possible gastroenteritis outbreak in a hotel located in northern Majorca was reported on June 2009. The subsequent investigation revealed a total of 14 cases with onset of symptoms from 18 June to 26 June. Symptoms affected mainly the children, their parents and the staff related to the children's club; a vomiting episode was described at the beginning of the outbreak. Genotype 2 norovirus was detected in stool samples, demonstrating its role as the aetiological agent. The special hygienic measures implemented allowed the outbreak to be controlled.

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Foodborne outbreak of gastroenteritis due to Norovirus and Vibrio parahaemolyticus

Cited by 38 publications

References 14 publications

Proteomic identification of membrane proteins regulating antimicrobial peptide resistance inVibrio parahaemolyticus

Proteomic identification of membrane proteins regulating antimicrobial peptide resistance inVibrio parahaemolyticus

Proteomic analysis reveals responsive proteins of Vibrio parahaemolyticus on exposure to cationic antimicrobial peptides

Gastroenteritis outbreak caused by norovirus associated with the children's club of a hotel located in Majorca, Spain

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