Foodomic investigations of food allergies

Anđelković, Uroš; Martinović, Tamara; Josić, Djuro

doi:10.1016/j.cofs.2015.06.003

Cited by 16 publications

(18 citation statements)

References 51 publications

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“…5 hypersensitivity such as allergic contact dermatitis [4,6]. Still valid hypothesis is that the gastrointestinal tract as the largest immunological organ is no more able to develop oral tolerance to a food allergen, or this tolerance has broken down as a result of influence of different factors.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Omics methods as a tool for investigation of food allergies

Andjelković

Gavrović-Jankulović

Martinović

et al. 2017

TrAC Trends in Analytical Chemistry

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Use of foodomics, mostly proteomic and genomic based methods, for study of allergens in food is presented. Immunological methods and nucleic acid-based methods are still most frequently used for diagnosis of allergies and for qualitative and quantitative determination of food allergens. They are sensitive, and can be used for the determination of allergens in trace concentrations. However, lack of specificity and cross-reaction of some antibodies can still be a relevant source of bias. The epitopes of protein allergens with posttranslational modifications and their changes originated during food processing cannot be traced by use of nucleic acid-based strategies. Recent developments of both antibody and nucleic acid-based biosensors, their miniaturization and increasing application of nanotechnology, significantly supported further use of both strategies. Regarding accuracy, reliability and sensitivity, mass spectrometry-based methods bring important advantage over both above presented strategies. Furthermore, the increasing use of mass spectrometry (MS) is discussed. Combined with proper sample preparation, liquid chromatography (LC) and/or different electrophoretic methods, targeted approach in mass spectrometry-based allergen analysis brings an additional strategic advance. However, MS is still rarely used for high-throughput analyses and detection and quantification of allergens for the reasons of price and relatively long time necessary for analysis. Recent developments of new high-resolution instruments are encouraging and enable development in the direction of a highthroughput strategy. Consequently, fast, very sensitive, reliable and accurate detection and quantification of allergens in highly complex samples such as food matrices, and the use of MS in routine determination of allergens can be reached in near future.

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Section: Accepted Manuscriptmentioning

confidence: 99%

“…The obligation of labeling potentially allergenic foods (e.g. European directive 2007/68/EC) is an important regulatory step which helps individuals at risk to make the right nutritional choice [6,7]. However, some accidents can still occur.…”

Section: Introductionmentioning

confidence: 99%

Omics methods as a tool for investigation of food allergies

Andjelković

Gavrović-Jankulović

Martinović

et al. 2017

TrAC Trends in Analytical Chemistry

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“…At the same time, hybrid type mass spectrometers were used for investigation of proteins in milk as putative biomarkers of milk contamination (208) and antibiotic resistance of potential foodborne pathogens (209,210). Picariello et al (211) and Andjelkovic et al (19) reviewed the use of mass spectrometry-based techniques for the determination of allergens in food. A comprehensive overview of applied techniques and instruments that can also be used for the determination of other quality and toxicity markers in food was given (like the above-mentioned isotope dilution MALDI-TOF MS).…”

Section: Hybrid Mass Spectrometers -Orbitrap and Other Instrumentsmentioning

confidence: 99%

“…These techniques are being increasingly applied in food analysis, as well as in food monitoring during harvest, processing, storage and transportation, until fi nal consumption. Moreover, they can be used for identifying possible agents of food poisoning or food allergies (2,(4)(5)(6)(18)(19)(20). In both contemporary food science and nutrition, new terms such as allergomics, nutrigenomics, nutrigenetics, nutriprote omics, nutrimetabolomics and similar are frequently used.…”

Section: Introductionmentioning

confidence: 99%

“…Investigation of allergies and detection of allergens in food is also a focus of foodomic investigations (19,23). This paper gives an overview of recent developments in foodomics as a new discipline that summarizes all high-throughput 'omic' techniques applied in food analysis (5,18,19), and their use in early, rapid, safe and reliable identifi cation of possible hazards, such as foodborne pathogens, toxins and other harmful components that can be present in food, or can occur as contaminants during food processing and distribution.…”

Section: Introductionmentioning

confidence: 99%

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Foodomics and Food Safety: Where We Are

Andjelković

Gajdošik

Gašo-Sokač

et al. 2017

Food Technol. Biotechnol.

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SummaryThe power of foodomics as a discipline that is now broadly used for quality assurance of food products and adulteration identifi cation, as well as for determining the safety of food, is presented. Concerning sample preparation and application, maintenance of highly sophisticated instruments for both high-performance and high-throughput techniques, and analysis and data interpretation, special att ention has to be paid to the development of skilled analysts. The obtained data shall be integrated under a strong bioinformatics environment. Modern mass spectrometry is an extremely powerful analytical tool since it can provide direct qualitative and quantitative information about a molecule of interest from only a minute amount of sample. Quality of this information is infl uenced by the sample preparation procedure, the type of mass spectrometer used and the analyst's skills. Technical advances are bringing new instruments of increased sensitivity, resolution and speed to the market. Other methods presented here give additional information and can be used as complementary tools to mass spectrometry or for validation of obtained results. Genomics and transcriptomics, as well as affi nity-based methods, still have a broad use in food analysis. Serious drawbacks of some of them, especially the affi nity-based methods, are the cross-reactivity between similar molecules and the infl uence of complex food matrices. However, these techniques can be used for pre-screening in order to reduce the large number of samples. Great progress has been made in the application of bioinformatics in foodomics. These developments enabled processing of large amounts of generated data for both identifi cation and quantifi cation, and for corresponding modeling.

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Comprehensive peptide marker identification for the detection of multiple nut allergens using a non-targeted LC–HRMS multi-method

2016

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Food allergies have emerged as a global problem over the last few decades; therefore, reliable and sensitive analytical methods to ensure food safety for allergic consumers are required. The application of mass spectrometry is of growing interest in this field and several procedures based on low resolution tandem mass spectrometry using single tryptic peptides as analytical targets have recently been described. However, a comprehensive survey of marker peptides for the development of multi-methods is still missing, as is a consensus guide to marker identification. In this study, we therefore report a consistent approach to the development of liquid chromatography-mass spectrometry (LC-MS) multi-screening methods for the detection of allergens in food matrices. Proteotypic peptides were identified by a shotgun proteomics approach and verified through a thorough investigation of specificity and sensitivity. On the basis of this procedure, we identified 44 suitable tryptic marker peptides from six allergenic nut species and developed the first analytical LC-MS method for the detection of trace nut contaminations in processed foods using high resolution mass spectrometry (HRMS). The analysis of spiked matrix samples gave limits of detection (LODs) below 10 μg/g for several nuts; these LODs are comparable with routinely used methods such as ELISA and PCR. Notably, the HRMS approach can be used in an untargeted fashion to identify multiple allergens also retrospectively. In conclusion, we present here the so far largest consensus set of analytical markers from nut allergens and to the best of our knowledge the first multi-allergen method based on LC-HRMS.

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Foodomic investigations of food allergies

Cited by 16 publications

References 51 publications

Omics methods as a tool for investigation of food allergies

Omics methods as a tool for investigation of food allergies

Foodomics and Food Safety: Where We Are

Comprehensive peptide marker identification for the detection of multiple nut allergens using a non-targeted LC–HRMS multi-method

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