Foot-and-Mouth Disease in the Middle East Caused by an A/ASIA/G-VII Virus Lineage, 2015–2016

Bachanek‐Bankowska, Katarzyna; Nardo, Antonello Di; Wadsworth, Jemma; Henry, Elisabeth; Parlak, Ünal; Тимина, А. М.; Mischenko, A. V.; Qasim, Ibrahim Ahmad; Abdollahi, Darab; Sultana, Munawar; Hossain, M. Anwar; King, Donald P.; Knowles, Nick J.

doi:10.3201/eid2406.170715

Cited by 26 publications

(41 citation statements)

References 14 publications

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“…High evident rRT-PCR results associated with samples that had CT values above the diagnostic threshold CT was sufficient for the confirmation of FMDV prevalence, this study had a beneficial result where FMD was endemic. Also, rRT-PCR able to identify and characterized different FMDV serotypes causes outbreaks (Bachanek-Bankowska et al, 2018). RRT-PCR results was similar to the result obtained by 3ABC ELISA and RT PCR.…”

Section: Resultssupporting

confidence: 78%

Diagnosis of Foot and Mouth Disease in Cattle and Buffaloes in Different Governorates of Egypt.

Zeedan¹,

Mahmoud²,

Abdalhamed³

et al. 2020

jwpr

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Foot and Mouth Disease (FMD) is highly contagious disease affected cloven-hoofed animals which result in substantial economic losses. The present study was aimed to detect FMDV by different serological and molecular methods in cattle and buffaloes for providing an accurate and rapid diagnosis of FMD disease. 86 samples of tongue epithelium biopsies, fluid vesicles samples and saliva, as well as 86 coagulated and uncoagulated blood samples, were collected from 64 and 22 suspected cattle and buffaloes respectively in different governorates in Egypt, during August to December 2017. Serum samples were examined by 3ABC-ELISA for differentiating between infected and non-infected animals. While tissues biopsies and un-coagulated blood samples were examined by Sandwich ELISA, Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) as well as Real-Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR). FMDV porotypes were identified by rRT-PCR in suspected cattle and buffaloes samples to FMDV serotype A, O and SAT2 and results showed that 54 samples positive for FMDV different serotypes while FMDV serotype differentiation in tissues biopsy of cattle were 18 (28.12%), 12 (18.75%), 3 (4.68 %) and 4 (6.25%). Also, the positive results of tissue samples from buffaloes examined by RT-PCR were 9 (40.09 %), 4 (6.25%), 2 (9.09 %) and 2 (9.09 %) for O, SAT2, serotype A and mixed serotypes respectively by different tests. The rRT-PCR provided an accurate and rapid laboratory diagnosis of FMDV as well as RT-PCR, and 3ABC-ELISA were given nearly the same results. Although the rRT-PCR generated results in less than 6 h and this is an important feature when definitive diagnostic results required in a short timescale during emergencies. Also, this study demonstrated the current situation of circulation FMDV type A, O, and SAT2 serotypes in cattle and buffaloes in Egypt.

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Section: Resultssupporting

confidence: 78%

Diagnosis of Foot and Mouth Disease in Cattle and Buffaloes in Different Governorates of Egypt.

Zeedan¹,

Mahmoud²,

Abdalhamed³

et al. 2020

jwpr

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“…The isolates BAN/GA/ Sa-197/2013 (BAN-197) and The complete VP1-based phylogeny revealed all our isolates clustering within the same sublineage C under the lineage A/ASIA/ G-VII (Figure 1). The findings of the reconstructed phylogenetic tree corroborated with the previous studies (Al Bachanek-Bankowska et al, 2018;Nandi et al, 2015). The field isolates BAN-307 and BAN-310 have 99% and 97% VP1 coding sequence identity, respectively, with BAN-304 (the field isolate used as a vaccine strain in this study).…”

Section: Circulating Fmdvs Belong To the Sublineage A/ Asia/g-vii Csupporting

confidence: 89%

Evidence of combined effect of amino acid substitutions within G‐H and B‐C loops of VP1 conferring serological heterogeneity in foot‐and‐mouth disease virus serotype A

Islam

Rahman

Amin

et al. 2020

Transbounding Emerging Dis

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Foot-and-mouth disease virus (FMDV) serotype A exhibits a higher degree of genetic and antigenic diversity resulting in frequent vaccine failure due to serological mismatch between the vaccine and heterologous strains. Currently, knowledge on the molecular basis of antigenic relationships among the FMDVs is limited; nevertheless, intratype antigenic variation due to mutation(s) is widely considered as the main hurdle to appropriate FMD vaccine development. Here, we studied genetic and antigenic variations of four FMDV serotype A isolates, BAN/GA/ Sa-197/2013 (BAN-197), BAN/CH/Sa-304/2016 (BAN-304), BAN/DH/Sa-307/2016 (BAN-307) and BAN/DH/Sa-310/2017 (BAN-310) circulating in Bangladesh during 2013-2017.Initially, antigenic relationships (r 1 -values) of the field isolates were evaluated by the two-dimensional microneutralization test (2D-MNT) using the hyperimmune antisera raised in cattle against the vaccine strain, BAN-304. Interesingly, the results showed protective serological cross-reactivity (r 1 -values > 0.4) between the vaccine strain and the field isolates, that substantially mismatched (r 1 = 0.129 ± 0.043) with the BAN-304. Although VP1-based phylogeny grouped all the isolates within the same sublineage C (a subgroup of VP3 Δ59 vari-

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“…In 2015, FMDV serotype A viruses from the A/ASIA/G-VII lineage (further denoted as G-VII), were detected in a number of countries in the Middle East. Sequence analysis revealed a close genetic relationship (>95% VP1 nucleotide identity) between viruses recovered from these outbreaks to FMD viruses circulating in South Asian countries, such as India and Bangladesh [3], and also demonstrated that these viruses were distinct (<84% VP1 nucleotide identity) from the A/ASIA/Iran-05 viruses that are endemic in the Middle East [4].…”

Section: Introductionmentioning

confidence: 88%

Cross-Protection Induced by a A/MAY/97 Emergency Vaccine Against Intra-Serotype Heterologous Challenge with a Foot-and-Mouth Disease Virus from the A/ASIA/G-VII Lineage

et al. 2020

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Since 2015, outbreaks of foot-and-mouth disease (FMD) in the Middle East have been caused by a new emerging viral lineage, A/ASIA/G-VII. Invitro vaccine matching data indicated that this virus poorly matched (low r 1 -value) with vaccines that were being used in the region as well as most other commercially available vaccines. The aim of this study was to assess the performance of two candidate vaccines against challenge with a representative field virus from the A/ASIA/G-VII lineage. The results from an initial full dose protection study provided encouraging data for the A/MAY/97 vaccine, while the A 22 /IRQ/64 vaccine only protected 2/7 vaccinated animals. In view of these promising results, this vaccine was tested in a potency test (PD 50 ) experiment in which 5 cattle were vaccinated with a full dose, 5 cattle with a 1/3 dose and 5 cattle with a 1/9 dose of vaccine. At 21 days post vaccination these vaccinated cattle and 3 control cattle were challenged intradermolingually with a field isolate from the A/ASIA/G-VII lineage. The intra-serotype heterologous potency test resulted in an intra-serotype heterologous potency of 6.5 PD 50 /dose. These data support previous studies showing that a high potency emergency vaccine can protect against clinical disease when challenged with a heterologous strain of the same serotype, indicating that not only the r 1 -value of the vaccine, but also the homologous potency of a vaccine should be taken into account when advising vaccines to control an outbreak.

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Foot-and-Mouth Disease in the Middle East Caused by an A/ASIA/G-VII Virus Lineage, 2015–2016

Cited by 26 publications

References 14 publications

Diagnosis of Foot and Mouth Disease in Cattle and Buffaloes in Different Governorates of Egypt.

Diagnosis of Foot and Mouth Disease in Cattle and Buffaloes in Different Governorates of Egypt.

Evidence of combined effect of amino acid substitutions within G‐H and B‐C loops of VP1 conferring serological heterogeneity in foot‐and‐mouth disease virus serotype A

Cross-Protection Induced by a A/MAY/97 Emergency Vaccine Against Intra-Serotype Heterologous Challenge with a Foot-and-Mouth Disease Virus from the A/ASIA/G-VII Lineage

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