Foot mucus and periostracum fraction as non-destructive source of DNA in the land snail Arianta arbustorum, and the development of new microsatellite loci

Armbruster, Georg F. J.; Koller, Bernhard; Baur, Bruno

doi:10.1007/s10592-004-7823-9

Cited by 27 publications

(28 citation statements)

References 14 publications

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“…owl pellets; Taberlet and Fumagalli 1996) can be sampled without significant damage to any part of the specimen. Even more peculiar sources of DNA have been identified: e.g., beachwashed shells of squids (Strugnell et al 2006), scent marks of hyaenas (Malherbe et al 2009), the foot mucus of snails (Armbruster et al 2005), and the body mucus of fish (Lucentini et al 2006) all have been used successfully for molecular genetic investigations. Moreover, Dalén et al (2007) were able to recover fox DNA from fresh footprints in snow.…”

Section: General Considerations For Establishing Archival Tissue Collmentioning

confidence: 99%

A hands-on overview of tissue preservation methods for molecular genetic analyses

2010

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Section: General Considerations For Establishing Archival Tissue Collmentioning

confidence: 99%

A hands-on overview of tissue preservation methods for molecular genetic analyses

2010

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“…We screened the DNA of mother snails of this population for microsatellite repeats using the nine primer pairs developed by Armbruster et al (2005) and tested in a previous study (Kupfernagel et al 2010). Primers that did not amplify or produced only a small number of alleles were not considered.…”

Section: Paternity Analysesmentioning

confidence: 99%

Sperm utilization in subadult and adult simultaneous hermaphrodite snails mating in the wild

KupfernagelSandra

BaurBruno

2011

Can. J. Zool.

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In species with multiple mating and long-term sperm storage, males are expected to show a preference for mating with virgin and young females to reduce the risk of sperm competition. In various simultaneous hermaphrodite land snail species, sperm production precedes egg production by 2-4 weeks, resulting in a short period of protandric hermaphroditism before shell growth is completed. In a natural population, we collected copulating pairs of the simultaneous hermaphrodite land snail Arianta arbustorum (L., 1758) consisting either of two adults, of two subadults, or of one adult and one subadult snail, and determined the paternity of their hatchlings that emerged from subsequently deposited eggs. Adult snails used sperm received from subadult mating partners for egg fertilization in the same frequency as sperm from adults, indicating that subadult and adult snails do not differ in male function. Furthermore, an unfinished shell is not a reliable indicator for virginity, because 35% of the subadult individuals had already sperm stored from previous mating(s). Compared with adults, young individuals exhibited a lower risk of sperm competition, indicated by a higher last mate sperm precedence. However, subadult snails produced fewer eggs than adult snails, counteracting the evolutionary advantage of preferring a young partner with low sperm competition risk.Résumé : Chez les espèces à accouplements multiples et à stockage à long terme des spermatozoïdes, on s'attend à ce que les mâles montrent une préférence pour l'accouplement avec des femelles jeunes et vierges afin de réduire le risque de compétition spermatique. Chez divers escargots terrestres à hermaphroditisme simultané, la production de spermatozoïdes pré-cède la production des oeufs de 2-4 semaines, ce qui résulte en une période d'hermaphroditisme protandrique avant la fin de la croissance de la coquille. Nous avons prélevé dans une population naturelle des paires accouplées de l'escargot terrestre à hermaphroditisme simultané Arianta arbustorum (L., 1758) comprenant ou bien deux adultes, ou deux subadultes ou alors un adulte et un subadulte; nous avons ensuite déterminé la paternité des nouveau-nés issus des oeufs qui ont été subsé-quemment déposés. Les escargots adultes utilisent le sperme reçu de leurs partenaires d'accouplement subadultes à la même fréquence que le sperme provenant des adultes, ce qui indique qu'il n'y a pas de différence dans la fonction mâle entre les subadultes et les adultes. De plus, une coquille incomplète n'est pas un indicateur fiable de virginité, puisque 35 % des individus subadultes possèdent déjà du sperme accumulé à partir d'un ou plusieurs d'accouplements antérieurs. Par rapport aux adultes, les jeunes affichent un risque plus faible de compétition spermatique, ce qui est indiqué par une préséance plus forte du sperme du dernier partenaire. Cependant, les escargots subadultes produisent moins d'oeufs que les adultes, ce qui contrecarre l'avantage évolutif qu'il y a à préférer un partenaire jeune à faible risque de compétiti...

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“…PCR was performed in 25 ll volume following the protocol described by Armbruster et al (2007b). Spreadex EL-400 or EL-600 gels (Elchrom Scientific AG, Switzerland) were used to resolve allelic patterns by horizontal electrophoresis performed on SEA 2000 advanced submerged gel electrophoresis equipment using M3 size marker (Elchrom Scientific AG, Switzerland; see Armbruster et al 2005).…”

Section: Dna Extraction and Microsatellite Amplificationmentioning

confidence: 99%

High population differentiation in the rock-dwelling land snail (Trochulus caelatus) endemic to the Swiss Jura Mountains

et al. 2009

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Understanding patterns of genetic structure is fundamental for developing successful management programmes for isolated populations of threatened species. Trochulus caelatus is a small terrestrial snail endemic to calcareous rock cliffs in the Northwestern Swiss Jura Mountains. Eight microsatellite loci were used to assess the effect of habitat isolation on genetic population structure and gene flow among nine populations occurring on distinct cliffs. We found a high genetic differentiation among populations (mean F ST = 0.254) indicating that the populations are strongly isolated. Both allelic richness and effective population size were positively correlated with the size of the cliffs. Our findings support the hypothesis that T. caelatus survived on ice-free cliffs during the Pleistocene glacier advancements from the Alps. Due to the establishment of beech and pine forest under recent, temperate climate conditions, dispersal between cliffs is no longer possible for rock-dwelling snails such as T. caelatus. Our results provide basic data for developing a conservation action plan for this endangered gastropod species.

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Foot mucus and periostracum fraction as non-destructive source of DNA in the land snail Arianta arbustorum, and the development of new microsatellite loci

Cited by 27 publications

References 14 publications

A hands-on overview of tissue preservation methods for molecular genetic analyses

A hands-on overview of tissue preservation methods for molecular genetic analyses

Sperm utilization in subadult and adult simultaneous hermaphrodite snails mating in the wild

High population differentiation in the rock-dwelling land snail (Trochulus caelatus) endemic to the Swiss Jura Mountains

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