Anthropogenic modification of the water cycle involves a diversity of processes, many of which have been studied intensively using models and observations. Effective tools for measuring the contribution and fate of combustion-derived water vapor in the atmosphere are lacking, however, and this flux has received relatively little attention. We provide theoretical estimates and a first set of measurements demonstrating that water of combustion is characterized by a distinctive combination of H and O isotope ratios. We show that during periods of relatively low humidity and/or atmospheric stagnation, this isotopic signature can be used to quantify the concentration of water of combustion in the atmospheric boundary layer over Salt Lake City. Combustion-derived vapor concentrations vary between periods of atmospheric stratification and mixing, both on multiday and diurnal timescales, and respond over periods of hours to variations in surface emissions. Our estimates suggest that up to 13% of the boundary layer vapor during the period of study was derived from combustion sources, and both the temporal pattern and magnitude of this contribution were closely reproduced by an independent atmospheric model forced with a fossil fuel emissions data product. Our findings suggest potential for water vapor isotope ratio measurements to be used in conjunction with other tracers to refine the apportionment of urban emissions, and imply that water vapor emissions associated with combustion may be a significant component of the water budget of the urban boundary layer, with potential implications for urban climate, ecohydrology, and photochemistry. A nthropogenic perturbation of the atmospheric water cycle is expressed over a wide range of spatial and temporal scales. Recent global changes resulting from warming-associated increases in saturation vapor pressure have been observed in satellite and reanalysis data (1, 2). Regional impacts related to large-scale land use change are detectible in precipitation data and models (3-5). Humidity anomalies (both positive and negative) have been observed in many urban centers and associated with changes in land cover, direct anthropogenic sources, and interaction of evapotranspiration and condensation processes with the urban heat island effect (6-10).Fossil fuel combustion releases water vapor to the atmosphere. Assuming an average molar ratio of H 2 O to CO 2 emission of 1.5 (see Water of Combustion) and current anthropogenic carbon emission rates of 9.5 Pg C/y (11), global combustion vapor emissions total âź21 Pg/y. At the global scale, these numbers are four orders of magnitude smaller than the gross global exchange of water vapor between the Earth surface and the atmosphere, which totals more than 480,000 Pg/y (12). However, anthropogenic emissions are highly concentrated in space and time and, locally, may be a significant source of vapor and impact atmospheric water cycling, ambient humidity, and photochemistry. Water of combustion has been hypothesized to be an important contributor...