Recent years have brought an important increase in the interest in identified human osteological collections in South America. Their number has been systematically growing and their sizes have been expanding, allowing the development of the field of forensic anthropology, among other disciplines, in this region. These collections are used mainly for the validation of international forensic anthropology methods for national and local populations, as well as for the training of professionals and researchers. Despite their growth, important limitation related to the lack of variability in the representativeness of individuals within these collections represents a significant drawback. Likewise, their concentration in only a few countries constitutes a noteworthy concern. This article aims at mapping the existing identified human osteological collections in South America, discussing the advances in the area of forensic anthropology that they have allowed, and reflecting upon their ethical dimensions in the South American context.