Force-controlled automatic microassembly of tissue engineering scaffolds

Zhao, Guoyong; Teo, Chee Leong; Hutmacher, Dietmar W.; Burdet, Etienne

doi:10.1088/0960-1317/20/3/035001

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…As a promising solution, bioassembly has emerged to orderly assemble biomimetic micromodules into vascular-like structures [4]. For instance, rapid prototyping assembles tissues by patterning the cellladen micromodules layer by layer [5,6]. Although the patterned micromodules are orderly stacked, it is not easy to spatially control every layer to create sophisticated structures in a microvessel.…”

Section: Introductionmentioning

confidence: 99%

Contact assembly of cell-laden hollow microtubes through automated micromanipulator tip locating

Wang¹,

Shi²,

Guo³

et al. 2016

J. Micromech. Microeng.

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This paper presents an automated contact assembly method to fabricate a cell-laden microtube based on accurate locating of the micromanipulator tip. Essential for delivering nutrients in thick engineered tissues, a vessel-mimetic microtube can be precisely assembled through microrobotic contact biomanipulation. The biomanipulation is a technique to spatially order and immobilize cellular targets with high precision. However, due to image occlusion during contact, it is challenging to locate the micromanipulator tip for fully automated assembly. To achieve pixel-wise tracking and locating of the tip in contact, a particle filter algorithm integrated with a determined level set model is employed here. The model ensures precise convergence of the micromanipulator’s contour during occlusion. With the converged active contour, the algorithm is able to pixel-wisely separate the micromanipulator from the low-contrast background and precisely locate the tip with error around 1 pixel (2 µm at 4 × magnification). As a result, the cell-laden microtube is automatically assembled at six layers/min, which is effective enough to fabricate vessel-mimetic constructs for vascularization in tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Contact assembly of cell-laden hollow microtubes through automated micromanipulator tip locating

Wang¹,

Shi²,

Guo³

et al. 2016

J. Micromech. Microeng.

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“…Figure 1 The most widely used technique for manipulating small components on and below the order of tens of micrometers is the microgrippers and microprobes used in microelectro mechanical system processes. However, these manipulators require direct contact with the components, and their use often results in the components adhering to the manipulator [1][2][3][4]. A number of self-assembly methods have already been developed in attempts to resolve the stiction problem.…”

Section: Introductionmentioning

confidence: 99%

Traceable assembly of microparts using optical tweezers

Kim

Hwang

Lee

2012

J. Micromech. Microeng.

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Assembly of components with a size in the order of tens of micrometers or less is difficult because the gravitational forces become smaller than weak forces such as capillary, electrostatic and van der Waals forces. As such, the picked-up components commonly adhere to the manipulator, making the release operation troublesome, and the repeatable supply of components cannot be guaranteed because the magazining and bunkering scheme available in conventional scale assembly cannot be extended to these small objects. Moreover, there are also no effective ways known to deliver the finalized assembly externally. In this paper, we present the manipulation and assembly of microparts using optical tweezers, which by nature do not have stiction problems. Techniques allowing bunkering and finalizing the assembly for exporting are also presented. Finally, we demonstrate an exemplary microassembly formed by assembling two microparts: a movable microring and a microrod fixed on a glass substrate. We believe this traceable microassembly to be an important step forward for micro-and nano-manufacturing.

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“…, 2010) and also for use as scaffolds in tissue‐engineered medical products (Mladenosvska et al. , 2007; Zhao et al. , 2010).…”

Section: Introductionmentioning

confidence: 99%

Effects of encapsulation on the viability of probiotic strains exposed to lethal conditions

Borges

Barbosa

Camilo

et al. 2011

Int J of Food Sci Tech

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The effect of microencapsulation on the viability of Lactobacillus casei, L. paracasei, L. acidophilus Ki and Bifidobacterium animalis BB-12 during exposure to lethal conditions (25% NaCl, pH 3.0 and 55-60°C) was evaluated. Results demonstrated that survival of probiotic strains to the imposed lethal stress conditions was strain dependent. With the exception of exposure to 25% (w ⁄ v) NaCl, L. acidophilus Ki (free and encapsulated cells) demonstrated the highest survival rates through exposure to lethal conditions of temperature and pH. For this probiotic strain exposed to heat, microencapsulated cells expressed a higher heat tolerance at 55°C than free cells. For the other tested bacteria, in general, encapsulation had no positive effect on survival through the tested lethal conditions.

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Force-controlled automatic microassembly of tissue engineering scaffolds

Cited by 7 publications

References 24 publications

Contact assembly of cell-laden hollow microtubes through automated micromanipulator tip locating

Contact assembly of cell-laden hollow microtubes through automated micromanipulator tip locating

Traceable assembly of microparts using optical tweezers

Effects of encapsulation on the viability of probiotic strains exposed to lethal conditions

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