“Force-from-lipids” gating of mechanosensitive channels modulated by PUFAs

Ridone, Pietro; Grage, Stephan L.; Patkunarajah, Amrutha; Battle, Andrew R.; Ulrich, Anne S.; Martinac, Boris

doi:10.1016/j.jmbbm.2017.12.026

Cited by 44 publications

(45 citation statements)

References 55 publications

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“…It is easily imaginable that mechanically-activated channels like Piezo could be affected by the surrounding membrane properties. Their activity have already been shown to be highly dependent on the membrane stiffening [58] and thus the membrane chol content, but also on the level of fatty acid saturation [59]. Moreover, new insights in Piezo1 structure evidenced a bend in its transmembrane section [60], whose stabilization energy might be partly compensated by the surrounding lipids.…”

Section: Lipid Domains As Modulators Of Piezo1 and Pmca Membrane Locamentioning

confidence: 98%

Spatial Relationship and Functional Relevance of Three Lipid Domain Populations at the Erythrocyte Surface

Conrard

Stommen

Cloos

et al. 2018

Cell Physiol Biochem

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Background/Aims: Red blood cells (RBC) have been shown to exhibit stable submicrometric lipid domains enriched in cholesterol (chol), sphingomyelin (SM), phosphatidylcholine (PC) or ganglioside GM1, which represent the four main lipid classes of their outer plasma membrane leaflet. However, whether those lipid domains co-exist at the RBC surface or are spatially related and whether and how they are subjected to reorganization upon RBC deformation are not known. Methods: Using fluorescence and/or confocal microscopy and well-validated probes, we compared these four lipid-enriched domains for their abundance, curvature association, lipid order, temperature dependence, spatial dissociation and sensitivity to RBC mechanical stimulation. Results: Our data suggest that three populations of lipid domains with decreasing abundance coexist at the RBC surface: (i) chol-enriched ones, associated with RBC high curvature areas; (ii) GM1/PC/chol-enriched ones, present in low curvature areas; and (iii) SM/PC/chol-enriched ones, also found in low curvature areas. Whereas chol-enriched domains gather in increased curvature areas upon RBC deformation, low curvature-associated lipid domains increase in abundance either upon calcium influx during RBC deformation (GM1/PC/chol-enriched domains) or upon secondary calcium efflux during RBC shape restoration (SM/PC/chol-enriched domains). Hence, abrogation of these two domain populations is accompanied by a strong impairment of the intracellular calcium balance. Conclusion: Lipid domains could contribute to calcium influx and efflux by controlling the membrane distribution and/or the activity of the mechano-activated ion channel Piezo1 and the calcium pump PMCA. Whether this results from lipid domain biophysical properties, the strength of their anchorage to the underlying cytoskeleton and/or their correspondence with inner plasma membrane leaflet lipids remains to be demonstrated.

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Section: Lipid Domains As Modulators Of Piezo1 and Pmca Membrane Locamentioning

confidence: 98%

Spatial Relationship and Functional Relevance of Three Lipid Domain Populations at the Erythrocyte Surface

Conrard

Stommen

Cloos

et al. 2018

Cell Physiol Biochem

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“…The transbilayer pressure profile ranges from 1,000 atm around the lipid head groups to hundreds of atmospheres around the bilayer center (Gullingsrud and Schulten, 2004). Although the transbilayer pressure profile has usually been determined using computational simulations (Gullingsrud and Schulten, 2004;Bavi et al, 2016aBavi et al, , 2016b attempts have been made to measure it experimentally (Templer et al, 1998;Ridone et al, 2018). The calculated values of the intra-bilayer pressure range between 250 atm for monounsaturated (18:1) lipids to 350 atm for polyunsaturated (18:2 and 18:3) lipids, which are in very good agreement with pressures calculated analytically (Cantor, 1999;Gullingsrud and Schulten, 2004) and with pressures determined recently by NMR spectroscopy (Ridone et al, 2018).…”

Section: Mechanosensitive Ion Channels As Force Sensorsmentioning

confidence: 99%

Biophysical Principles of Ion-Channel-Mediated Mechanosensory Transduction

2019

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“…For GPCRs, publications on these effects are still comparably rare. However, many studies on other IMPs, including mechanosensitive ion channels [ 41 , 42 , 43 ] and membrane transporters [ 44 , 45 ], demonstrate that the lateral pressure could have important implications for GPCRs. It is by now established that GPCR activation includes both large scale structural rearrangements, as well as changes in the plasticity of the receptors [ 46 ].…”

Section: Interactions Of Gpcrs With Their Membrane Environmentmentioning

confidence: 99%

Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches

et al. 2020

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Over the past decade, the vast amount of information generated through structural and biophysical studies of GPCRs has provided unprecedented mechanistic insight into the complex signalling behaviour of these receptors. With this recent information surge, it has also become increasingly apparent that in order to reproduce the various effects that lipids and membranes exert on the biological function for these allosteric receptors, in vitro studies of GPCRs need to be conducted under conditions that adequately approximate the native lipid bilayer environment. In the first part of this review, we assess some of the more general effects that a membrane environment exerts on lipid bilayer-embedded proteins such as GPCRs. This is then followed by the consideration of more specific effects, including stoichiometric interactions with specific lipid subtypes. In the final section, we survey a range of different membrane mimetics that are currently used for in vitro studies, with a focus on NMR applications.

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“Force-from-lipids” gating of mechanosensitive channels modulated by PUFAs

Cited by 44 publications

References 55 publications

Spatial Relationship and Functional Relevance of Three Lipid Domain Populations at the Erythrocyte Surface

Spatial Relationship and Functional Relevance of Three Lipid Domain Populations at the Erythrocyte Surface

Biophysical Principles of Ion-Channel-Mediated Mechanosensory Transduction

Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches

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