Force-Sensing Robotic Microinjection System for Automated Multi-Cell Injection With Consistent Quality

Nan, Zhijie; Xu, Qingsong; Zhang, Hongjie; Ge, Wei

doi:10.1109/access.2019.2913592

Cited by 26 publications

(5 citation statements)

References 37 publications

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“…However, there are significant drawbacks for microinjection. Most notably post-injection cell survival rate is on average ∼50%, although this can be improved by automating the injection process [22,23]. One reason for the impact of microinjection on cells may be the large size of the micropipette's tip relative to the cell.…”

Section: Injection Of Cells: From Microscale To Nanoscale Probesmentioning

confidence: 99%

Methods for protein delivery into cells: from current approaches to future perspectives

Chau

Actis

Hewitt

2020

Biochemical Society Transactions

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The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into defined complexes and subject to biophysical analyses prior to their delivery into cells. Here, we review new approaches to the injection and electroporation of proteins into cultured cells. In particular, we focus on how recent developments in nanoscale injection probes and localized electroporation devices enable proteins to be delivered whilst minimizing cellular damage. Moreover, we discuss how nanopore sensing may ultimately enable the quantification of protein delivery at single-molecule resolution.

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Section: Injection Of Cells: From Microscale To Nanoscale Probesmentioning

confidence: 99%

Methods for protein delivery into cells: from current approaches to future perspectives

Chau

Actis

Hewitt

2020

Biochemical Society Transactions

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“…However, one microscope can only capture the images on a flat surface, some ''depth of field'' during injection cannot be accurately obtained [96], [97]. Thus, researchers have integrated microforce sensors to monitor the injection process and to improve the success rate of injection [92], [98]- [101]. For example, Nan et al [98] combined the pyramid template matching algorithm with Kalman filter, guided the microinjection location of cells with a visual sensor, and added a force sensor to determine the exact time of penetration into cells, thus achieving multi-cell automatic injection.…”

Section: Sensors In Cell Microinjectionmentioning

confidence: 99%

A Review of Recent Advances in Robotic Cell Microinjection

Chi

Zhu

2020

IEEE Access

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Cell microinjection is a direct and effective way to transfer external materials into the cell. In the field of modern biomedicine, cell microinjection is very important, no matter in genetics, reproductive health, tumor therapy or others related research directions. Traditional manual cell microinjection has the disadvantages of low efficiency and low survival rate. In contrast, robotic cell microinjection can achieve precise and effective cell microinjection, and can be used in the injection of large quantities of cells. This paper reviews recent advances in robotic cell microinjection technologies. It summarizes the main approaches of key technologies and their advantages and disadvantages, such as cell identification, cell holding, precise positioning platform, cell injection strategy, sensors in cell microinjection, and cell modeling. The conclusion of the investigation is that the robotic cell microinjection has achieved remarkable results and still has great potential for further development. It is expected to be widely used in biomedicine and other fields to realize convenient, fast, and efficient microinjection operations of large number of individual cells. INDEX TERMS Robotic cell microinjection, cell identification, cell holding, precise displacement platform, injection strategy, sensors in cell microinjection, cell modeling.

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“…Recognition algorithms for zebrafish embryos and larvae have made significant progress. Some efforts use traditional visual processing methods to identify the microinjection target 43 , 44 , such as binarization, template matching, and contour extraction. Nevertheless, these methods are not sufficiently robust in terms of anti-interference, especially in environments containing miscellaneous matter.…”

Section: Introductionmentioning

confidence: 99%

Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae

Guo,

Ai,

et al. 2024

Microsyst Nanoeng

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This paper presents the design of a vision-based automated robotic microinjection system for batch injection of both zebrafish embryos and larvae. A novel visual recognition algorithm based on an automatic threshold and excessive dilatation is introduced to accurately identify the center of zebrafish embryos and larval yolks. A corresponding software system is developed using the producer-consumer model as the framework structure, and a friendly user interface is designed to allow operators to choose from a range of desired functions according to their different needs. In addition, a novel microstructural agarose device is designed and fabricated to simultaneously immobilize mixed batches of embryos and larvae. Moreover, a prototype microinjection system is fabricated by integrating hardware devices with visual algorithms. An experimental study is conducted to verify the performance of the robotic microinjection system. The results show that the reported system can accurately identify zebrafish embryos and larvae and efficiently complete batch microinjection tasks of the mixtures with an injection success rate of 92.05% in 13.88 s per sample. Compared with manual and existing microinjection systems, the proposed system demonstrates the merits of versatility, excellent efficiency, high success rate, high survival rate, and sufficient stability.

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Force-Sensing Robotic Microinjection System for Automated Multi-Cell Injection With Consistent Quality

Cited by 26 publications

References 37 publications

Methods for protein delivery into cells: from current approaches to future perspectives

Methods for protein delivery into cells: from current approaches to future perspectives

A Review of Recent Advances in Robotic Cell Microinjection

Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae

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