2008
DOI: 10.1007/s12013-008-9021-8
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Force–Velocity Curves of Motor Proteins Cooperating In Vivo

Abstract: Motor proteins convert chemical energy into work, thereby generating persistent motion of cellular and subcellular objects. The velocities of motor proteins as a function of opposing loads have been previously determined in vitro for single motors. These single molecule "force-velocity curves" have been useful for elucidating motor kinetics and for estimating motor performance under physiological loads due to, for example, the cytoplasmic drag force on transported organelles. Here we report forcevelocity curve… Show more

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Cited by 43 publications
(62 citation statements)
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“…[13] we obtained the values of D * by fitting the MSD-vs-time lag plot of each trajectory with the empirical expression given by Eq. (26). The distributions of D * followed an exponential behavior, with mean values equal to 2 × 10 −3 µm 2 and 4.4 × 10 −3 µm 2 for aggregation and dispersion conditions, respectively.…”
Section: Resultsmentioning
confidence: 88%
See 1 more Smart Citation
“…[13] we obtained the values of D * by fitting the MSD-vs-time lag plot of each trajectory with the empirical expression given by Eq. (26). The distributions of D * followed an exponential behavior, with mean values equal to 2 × 10 −3 µm 2 and 4.4 × 10 −3 µm 2 for aggregation and dispersion conditions, respectively.…”
Section: Resultsmentioning
confidence: 88%
“…Recently, pioneering works developed new techniques that allow to measure the forces exerted by molecular motors directly [25,26]. In Ref.…”
Section: Introductionmentioning
confidence: 99%
“…Bead velocities were determined via a chi-squared minimization procedure reported in Ref. 24. Each plot is constructed from 7-27 trajectories (3-19 complexes).…”
Section: Resultsmentioning
confidence: 99%
“…Average bead velocities were determined using a linear regression procedure that is similar to those presented in prior reports (14,24). In this procedure, velocity segments are determined via a linear regression/chi-squared optimization procedure that both partitions traces into segments where beads moved with approximately constant velocities and determines bead velocities during these segments.…”
Section: Methodsmentioning
confidence: 99%
“…In particular, we emphasize the importance of the slowest time scale of the velocity autocorrelation in order to quantify phenomena related to, e.g., reversal of motion direction and cargo unbinding. These processes are of great biological interest since their properties suggest that many non-trivial physical and bio-chemical mechanisms are relevant for cargo transport [38,5,3,39,9,40,16,41].…”
Section: Discussionmentioning
confidence: 99%