Abstract:The deamination of
adenosine to inosine is an important modification
in nucleic acids that functionally recodes the identity of the nucleobase
to a guanosine. Current methods to analyze and detect this single
nucleotide change, such as sequencing and PCR, typically require time-consuming
or costly procedures. Alternatively, fluorescent “turn-on”
probes that result in signal enhancement in the presence of target
are useful tools for real-time detection and monitoring of nucleic
acid modification. Here we descri… Show more
“…14,20 As a result, FIT probes provide for single nucleotide specic uorescent signalling regardless of hybridization delity. This property has facilitated the real-time detection of single nucleotide alterations in in vitro assays 21,22 and uorescence microscopic imaging. [23][24][25][26][27][28][29][30] The FIT probe concept has also been applied for detection of double strands via triplex formation, [31][32][33] and in signalling aptamers.…”
Efficient fluorogenic hybridization probes combine high brightness and specificity of fluorescence signaling with large turn-on of fluorescence. Herein, we present an approach to enhance signaling by combining two identical fluorescence...
“…14,20 As a result, FIT probes provide for single nucleotide specic uorescent signalling regardless of hybridization delity. This property has facilitated the real-time detection of single nucleotide alterations in in vitro assays 21,22 and uorescence microscopic imaging. [23][24][25][26][27][28][29][30] The FIT probe concept has also been applied for detection of double strands via triplex formation, [31][32][33] and in signalling aptamers.…”
Efficient fluorogenic hybridization probes combine high brightness and specificity of fluorescence signaling with large turn-on of fluorescence. Herein, we present an approach to enhance signaling by combining two identical fluorescence...
Fluorogenic hybridization probes are essential tools in modern molecular biology techniques. They allow detection of specific nucleic acid molecules without the need to separate target-bound from unbound probes. To enable...
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