Forces affecting nanoparticles in fluid, and the shape of the acoustic signal accompanying particle detachment from QCM surface

Dultsev, F. N.; Kolosovsky, E.A.

doi:10.1016/j.snb.2014.05.031

Cited by 7 publications

(1 citation statement)

References 34 publications

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“…This raises a relevant question concerning the use of high frequency resonators as biosensors: what is the size of the species above which these forces have tobe considered? Some references in the literature dealing with the detection of whole bacteria with QCM address this issue [26,27]. A Thrombin concentration (nM) Fig.…”

Section: Sensor Calibrationmentioning

confidence: 99%

Gravimetric biosensor based on a 1.3 GHz AlN shear-mode solidly mounted resonator

DeMiguel-Ramos

Díaz-Durán

Escolano

et al. 2017

Sensors and Actuators B: Chemical

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We investigate the performance of solidly mounted resonators based on Ir/tilted-AlN/Ir piezoelectric stacks as biosensors. These films are deposited by varying the pressure, the cathode power and the temperature of a two-step process based on depositing (00 2)-til ted AIN active layers over an (10 3)-oriented AIN seed layer. To minimize the influence of the temperature coefficient of frequency on the stability of the biosensor, we use insulating acoustic mirrors made of layers of Si0 2 and amorphous TaO x with non-\/4 thicknesses, which enables to reduce the TCF to-14 ppm/°C. The mass loading of the resonators with SÍO2 thin films results in a sensitivity of 1800 kHz/pg cm 2. Surface functionalization consists on the binding of silane groups on plasma oxidized SÍO2 surfaces. After a glutaraldehyde link, streptavidin is bonded to the surface to receive biotinylated receptors for several species. We test thrombin-binding aptamer (TBA29 against thrombin, and IgG antibody against immunoglobulin). The sensors response to species of different molecular weight like TBA-29 (9.75 kDa) or IgG antibody (150 kDa) is monitored. Finally, we assess the response of the biosensors to different thrombin concentrations (ranging from 4 nM to 270 nM) on surfaces functionalized with the TBA29 aptamer.

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Section: Sensor Calibrationmentioning

confidence: 99%

Gravimetric biosensor based on a 1.3 GHz AlN shear-mode solidly mounted resonator

DeMiguel-Ramos

Díaz-Durán

Escolano

et al. 2017

Sensors and Actuators B: Chemical

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Microtubules: Nanobiomechanical Simulation

Motamedi

2018

Computational Approaches in Biomedical Nano‐Engineering

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QCM-based rupture force measurement as a tool to study DNA dehybridization and duplex stability

et al. 2016

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The stability of double-stranded DNA (dsDNA) was assessed on the basis of unwinding force measurement. Unwinding force was measured directly with a quartz crystal microbalance (QCM). The amplitude of its surface oscillations was controlled by supplying variable alternate voltage. Under smoothly increasing amplitude of QCM surface oscillations, dsDNA fixed on QCM surface through one of its ends got unwound. This procedure allows reliable measurement of rupture force as small as 5-10 pN. It was demonstrated that oscillations of the surface, with dsDNA bound through one of its ends to this surface, at a frequency of 14 MHz, cause helix unwinding to form two complementary parts due to viscous forces of the liquid medium. Unwinding starts at the upper end. This was proven using oligonucleotide duplexes containing mismatches in different positions. For duplexes containing complementary 20 base pairs, the helix unwinding force is equal to 30-40 pN, which is in agreement with the data obtained by means of atomic-force microscopy (AFM) for the case of unzipping mode. Graphical Abstract Rupture force depending on mismatch position in dsDNA.

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Forces affecting nanoparticles in fluid, and the shape of the acoustic signal accompanying particle detachment from QCM surface

Cited by 7 publications

References 34 publications

Gravimetric biosensor based on a 1.3 GHz AlN shear-mode solidly mounted resonator

Gravimetric biosensor based on a 1.3 GHz AlN shear-mode solidly mounted resonator

Microtubules: Nanobiomechanical Simulation

QCM-based rupture force measurement as a tool to study DNA dehybridization and duplex stability

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