24 patients are selected and 30 teeth in vestibular mucosae, without keratinized gingiva. No active periodontal disease (no BOP and gingival recessions). No BOP and CAL loss at the start of the orthodontic treatment. Every patient was treated with self ligating appliances (Time2/Time3, American Orthodontics). In every orthodontic session (each 30 days as average) the patient was treated with Nd:Yag laser biostimulation, for 90 seconds, with 320 micron fiber section, on utilizing the Biostimulating machine paremeters . No termical effects were produced by lasers applications. At the moment of debonding, 30 teeth involved in the research were evaluated in terms of quality and quantity of attached gingiva. BOP and CAL loss were also investigated. Every tooth considerated, at the end of orthodontic treatment showed a attached gingiva around the crown: the average of keratinized gingiva at the end of the study was 2,7 mm and the mean increasing at each month was 0,45 mm .. The combination between self ligating appliances and laser's biostimulation could improve the differentiation of periodontal ligaments stem cells in fibroblasts, able to promote attached gingiva around the crown of the teeth erupted in oral vestibular mucosae.In orthodontic treatment, tooth movement is related to the response to applied orthodontic forces that cause remodeling of the periodontal tissues, especially alveolar bone. The typical 2-to 3-year treatment period is burdensome for patients, so it is very important to accelerate alveolar bone remodeling during treatment to abbreviate the time required.Recently, various biostimulatory effects oflow energy laser irradiation that involve wound healing and fibroblast proliferation, collagen synthesis, and nerve regeneration have been reported. (1-3) In particular, the acceleration of bone regeneration by laser treatment has been a focus of contemporary researches. Therefore, if laser irradiation can cause the acceleration of bone remodeling, it may also have great potential benefit in abbreviating the orthodontic treatment period. (1, 2) The present study was designed to examine the effects of low-energy laser irradiation on alveolar bone remodeling during orthodontic tooth movement and consequently formation of new keratinized gingiva.
LLLTLow level laser therapy (LLLT) is also known as "soft laser therapy" and bio-stimulation. The use ofLLLT in health care has been documented in the literature for more than 30 years and many research studies have demonstrated that LLLT is effective for some specific applications in dentistry. Recently, various effects of low-energy laser irradiation have been reported, including wound healing, fibroblast proliferation, collagen synthesis and nerve regeneration. In particular, the acceleration of bone regeneration by laser treatment has been the focus of recent studies.In orthodontics, low-energy laser irradiation can be utilized for several different treatments, such as the reduction of post-adj ustment pain, or treatment oftraumatic ulcers in the oral mucosa i...