Foreign Body in the Esophagus

Tinoco, Paulo; Ferreira, Flávia Regina; Carrara, Vânia Lúcia; Brito, Lara Bonani de Almeida; Tinoco, Marina Bandoli de Oliveira; Tinoco, Saulo

doi:10.7162/s1809-977720120s1po-121

Cited by 8 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PSGL-1-deficiency enhances T cell antitumor immunity to melanoma, promotes viral control and T cell survival is reported to be increased in PSGL-1 deficit T cells after chronic virus infection. 2,3 The proposed device also offers direct measurement of transient interactions between important physiological ligands and cells. The device can also be used as a tool to monitor stem cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Identifying and isolating cells that express desired molecular surface markers is thus critical to a variety of applications in the biological sciences, cell therapy, and medical diagnostics. [1][2][3][4] Label-free separation techniques that manipulate physical biomarkers, such as size, stiffness, and shape, to sort cells have been successfully demonstrated during the past decade, but often lack the specificity [5][6][7][8][9][10][11] that can be achieved by using cell surface biomarkers. Cell surface biomarkers are typically determined through adhesion-based cell isolation platforms to specifically purify by immunophenotype.…”

Section: Graphical Abstractmentioning

confidence: 99%

“…[26][27][28][29] We chose the specific lectin P selectin which binds to the P selectin glycoprotein ligand 1 (PSGL-1) to demonstrate sorting due to the potential applications in understanding the role of PSGL in T cell immune response 30,31 and developing effective therapeutics. 2,3,23,32 Therefore, in this study PSGL-1/P selectin, ligand-receptor binding is used to sort target cells.…”

Section: Graphical Abstractmentioning

confidence: 99%

See 2 more Smart Citations

Continuous Sorting of Cells Based on Differential P Selectin Glycoprotein Ligand Expression Using Molecular Adhesion

et al. 2017

View full text Add to dashboard Cite

Cell surface molecular adhesions govern many important physiological processes and are used to identify cells for analysis and purifications. But most effective cell adhesion separation technologies use labels or long-term attachments in their application. While label-free separation microsystems typically separate cells by size, stiffness, and shape, they often do not provide sufficient specificity to cell type that can be obtained from molecular expression. We demonstrate a label-free microfluidic approach capable of high throughput separation of cells based upon surface molecule adhesion. Cells are flowed through a microchannel designed with angled ridges at the top of the channel and coated with adhesive ligands specific to target cell receptors. The ridges slightly compress passing cells such that adhesive contact can be made with sufficient surface area without unduly affecting cell trajectories because of cell stiffness. Thus, sorting is sensitive to cell adhesion but not to stiffness or cell size. The enforced interactions between the cells and the ridges ensure that a high flow rate can be used without lift forces quenching adhesion. As a proof of principle of the method, we separate both Jurkat and HL60 cell lines based on their differential expression of PSGL-1 ligand by using a ridged channel coated with P selectin. We demonstrate 26-fold and 3.8-fold enrichment of PSGL-1 positive and 4.4-fold and 3.2-fold enrichment of PSGL-1 negative Jurkat and HL60 cells, respectively. Increasing the number of outlets to five allows for greater resolution in PSGL-1 selection resulting in fractionation of a single cell type into subpopulations of cells with high, moderate, and low PSGL-1 expression. The cells can flow at a rate of up to 0.2 m/s, which corresponds to 0.045 million cells per minute at the designed geometry, which is over 2 orders of magnitude higher than previous adhesive-based sorting approaches. Because of the short interaction time of the cells with the adhesive surfaces, the sorting method does not further activate the cells due to molecular binding. Such an approach may find use in label-free selection of cells for a highly expressed molecular phenotype.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Graphical Abstractmentioning

confidence: 99%

Section: Graphical Abstractmentioning

confidence: 99%

See 1 more Smart Citation

Continuous Sorting of Cells Based on Differential P Selectin Glycoprotein Ligand Expression Using Molecular Adhesion

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The study of animal behavior is essential because it brings contributions to other areas of knowledge such as neurobiology, human study, and environmental conservation since we are part of it 10 . Much like humans, animals subjected to the stress of everyday life, in a compulsive and untreated way, can have serious consequences, whether the animal is wild or domestic (pet) 11 .…”

Section: Bluementioning

confidence: 99%

Extracción Y Caracterización De Celulosa a Partir De La Planta Del Plátano

Arreola¹,

González²,

Barbosa³

et al. 2023

Meio Ambiente E Sustentabilidade

View full text Add to dashboard Cite

show abstract

“…39 "Shocks," such as recession and credit crunches, contribute to default by affecting firm assets or cash flow negatively. 43 42 Of these factors, financial or accounting measures of liquidity, solvency, leverage, profitability, asset composition, firm size, and growth rate are the best predictors of the incidence of default, followed by market-based variables such as interest rates and credit default swap rates, with macroeconomic variables such as GDP adding relatively little to improving predictive accuracy.…”

Section: Models Differing In Choice Of Variables Used To Predict Bankmentioning

confidence: 99%

Alternative Exit and Restructuring Strategies

DePamphilis

2015

Mergers, Acquisitions, and Other Restructuring Activities

View full text Add to dashboard Cite

Foreign Body in the Esophagus

Cited by 8 publications

References 0 publications

Continuous Sorting of Cells Based on Differential P Selectin Glycoprotein Ligand Expression Using Molecular Adhesion

Continuous Sorting of Cells Based on Differential P Selectin Glycoprotein Ligand Expression Using Molecular Adhesion

Extracción Y Caracterización De Celulosa a Partir De La Planta Del Plátano

Alternative Exit and Restructuring Strategies

Contact Info

Product

Resources

About