Forensic DNA Testing on Skeletal Remains from Mass Graves: A Pilot Project in Guatemala

Boles, T. Christian; Snow, Clyde C.; Stover, Eric

doi:10.1520/jfs13786j

Cited by 87 publications

(60 citation statements)

References 23 publications

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“…The maternal lineage affinity between the Chibchan and Maya groups was based on one contemporary Maya group from Guatemala (Boles et al 1995;Melton et al 2007Melton et al , 2013. However, this association has now been confirmed in this study analyzing the sequences of pre-Hispanic Mexican Maya and sequences from the Caribbean Islands, Colombia, and contemporary Maya groups from Mexico and Guatemala, as well as Central and South American Chibchan speakers from previous reports (Table 3).…”

Section: Network Analysis Of Haplogroup Asupporting

confidence: 81%

“…haplogroup B than haplogroup A (González-Martín et al 2015), and the ancient bones collected in a Quiché Indian village, located close to the provincial capital of Santa Cruz de Quiché, revealed the presence of 16 different mtDNA haplotypes, among which haplogroup B has the highest frequency (Boles et al 1995). The pre-Hispanic Maya population of this study displayed a high frequency of haplogroup A (60.53%), followed by haplogroup C (34.21%), a very low frequency of haplogroup D (5.26%), and the complete absence of haplogroup B.…”

Section: Mitochondrial Haplogroup Frequenciesmentioning

confidence: 99%

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Genetic Affiliation of Pre-Hispanic and Contemporary Mayas through Maternal Linage

Ochoa-Lugo¹,

Muñoz²,

Pérez-Ramírez³

et al. 2016

Human Biology

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Maya civilization developed in Mesoamerica and encompassed the Yucatan Peninsula, Guatemala, Belize, part of the Mexican states of Tabasco and Chiapas, and the western parts of Honduras and El Salvador. This civilization persisted approximately 3,000 years and was one of the most advanced of its time, possessing the only known full writing system at the time, as well as art, sophisticated architecture, and mathematical and astronomical systems. This civilization reached the apex of its power and influence during the Preclassic period, from 2000 BCE to 250 CE. Genetic variation in the pre-Hispanic Mayas from archaeological sites in the Mexican states of Yucatan, Chiapas, Quintana Roo, and Tabasco and their relationship with the contemporary communities in these regions have not been previously studied. Consequently, the principal aim of this study was to determine mitochondrial DNA (mtDNA) variation in the pre-Hispanic Maya population and to assess the relationship of these individuals with contemporary Mesoamerican Maya and populations from Asia, Beringia, and North, Central, and South America. Our results revealed interactions and gene flow between populations in the different archaeological sites assessed in this study. The mtDNA haplogroup frequency in the pre-Hispanic Maya population (60.53%, 34.21%, and 5.26% for haplogroups A, C, and D, respectively) was similar to that of most Mexican and Guatemalan Maya populations, with haplogroup A exhibiting the highest frequency. Haplogroup B most likely arrived independently and mixed with populations carrying haplogroups A and C based on its absence in the pre-Hispanic Mexican Maya populations and low frequencies in most Mexican and Guatemalan Maya populations, although this also may be due to drift. Maya and Ciboneys sharing haplotype H10 belonged to haplogroup C1 and haplotype H4 of haplogroup D, suggesting shared regional haplotypes. This may indicate a shared genetic ancestry, suggesting more regional interaction between populations in the circum-Caribbean region than previously demonstrated. Haplotype sharing between the pre-Hispanic Maya and the indigenous populations from Asia, the Aleutian Islands, and North, Maternal Lineage in Pre-Hispanic and Contemporary Mayas ■ 137Central, and South America provides evidence for gene flow from the ancestral Amerindian population of the pre-Hispanic Maya to Central and South America.

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Section: Network Analysis Of Haplogroup Asupporting

confidence: 81%

Section: Mitochondrial Haplogroup Frequenciesmentioning

confidence: 99%

Genetic Affiliation of Pre-Hispanic and Contemporary Mayas through Maternal Linage

Ochoa-Lugo¹,

Muñoz²,

Pérez-Ramírez³

et al. 2016

Human Biology

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show abstract

“… Sequence analysis of human mtDNA (mitochondrial DNA) has been demonstrated to be a valid and reliable tool for the genetic characterization of forensic biological specimens [1][2][3]. The human mtDNA is a 16569 np (nucleotide pair) closed, circular molecule located within the cytoplasmic mitochondria.…”

Section: Introductionmentioning

confidence: 99%

Identification of Human Remains Using mtDNA Sequence Analysis in Morocco

Amri¹,

Boutayeb²,

Jennouni³

et al. 2015

UCMS

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Today, mtDNA typing is utilized primarily in cases in which the nuclear DNA is too degraded or cannot be recovered in sufficient quantities to be typed. Laboratory of Genetic of Royal Gendarmerie in Morocco is actively involved in using mtDNA for forensic identifications of human skeletal remains. Reproducible results were obtained for bones and teeth up to 52 years old. The bone and tooth samples were pulverized to fine powder, decalcified and DNA was extracted. 341-bp fragment from HVI (hypervariable region I) and 267-bp fragment from HVII (hypervariable region II) of the mtDNA control region were amplified. After sequencing of the PCR products, mitotypes were compared to the rCRS (revised Cambridge Reference Sequence) and a phylogenetic tree was built.

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“…The SNPs that occur in the mitochondrial genome are very important tool in the species identification, forensic studies as well as anthropological and evolutionary research (Boles et al, 1995). Harumi et al (2004) observed the single nucleotide polymorphism in the D-loop region in chicken mitochondrial DNA and reported 11 SNPs.…”

Section: Introductionmentioning

confidence: 99%

Allele Specific PCR for Genotyping the Probable SNPs in Mitochondrial Genome in Red Jungle Fowl and Domestic Chicken

Ahmed

Sharma

2010

Journal of Applied Animal Research

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Forensic DNA Testing on Skeletal Remains from Mass Graves: A Pilot Project in Guatemala

Cited by 87 publications

References 23 publications

Genetic Affiliation of Pre-Hispanic and Contemporary Mayas through Maternal Linage

Genetic Affiliation of Pre-Hispanic and Contemporary Mayas through Maternal Linage

Identification of Human Remains Using mtDNA Sequence Analysis in Morocco

Allele Specific PCR for Genotyping the Probable SNPs in Mitochondrial Genome in Red Jungle Fowl and Domestic Chicken

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