Forensic parameters and genetic structure based on Y-chromosome short tandem repeats in Lesotho populations

Lesaoana, Mpasi; Kasu, Mohaimin; D’Amato, María Eugenia

doi:10.1016/j.fsigss.2019.10.033

Cited by 5 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A high level of allele diversity was observed among the studied populations as evident from the allelic richness (Ar) and haplotype and gene diversity values. These results agree with those obtained for Southern African populations (Lesaoana et al, 2019;Kasu et al, 2022) The Hausas harbor higher levels of allele richness or diversity than the other groups, which may be attributed to admixture, higher lineage diversity, marriage practices, or historical demographic processes. Allelic richness estimation is one of the various criteria for assessing within-and among-population genetic diversity and may also be used to estimate population evolutionary histories (Castric and Bernatchez, 2003;Ollivier and Foulley, 2005).…”

Section: Discussionsupporting

confidence: 89%

“…Duplications at the DYS626 locus are rare. The reported duplications at the DYS626 locus are unique and have not been encountered in previous studies with the same genotyping system in Lesotho (Lesaoana et al, 2019) and South African populations (Kasu et al, 2022). Ballantyne et al (2014) reported different duplications for this locus among Sub-Saharan (30,32 and 31,33),North African (31,32), admixed native American (30, 32), and European (29, 31) populations.…”

Section: Discussionmentioning

confidence: 48%

“…Hence, the present study was aimed at generating Y-STR haplotypes suitable for forensic and population analyses among Nigerian males using 10 Y-STR loci in order to gain more understanding of Y-STR landscape in the Nigerian diverse populations. This study utilized the UniQTyper TM Y-10 genotyping kit with improved discrimination capacities in African populations (D'Amato et al, 2011;Lesaoana et al, 2019;Kasu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Forensic parameters of 10 Y-STR markers in the Nigerian Hausa, Igbo, and Yoruba populations

Fakorede,

Adekoya,

Iroanya

et al. 2024

USci

View full text Add to dashboard Cite

Y chromosome short tandem repeats, or Y-STRs, are interesting genetic markers suitable for identifying male offenders in forensic investigations because of the haploid nature of the human male determining chromosome. Furthermore, due to the non-recombining nature of the Y chromosome, Y-STRs are choice markers in paternity or kinship analysis, human evolutionary investigations, and population studies. The lack of reference Y-haplotype data for the populace currently hampers the use of these markers in forensic applications in Nigeria. In this study, the 10 Y-STR loci in the UniQTyperTM Y-10 system including DYS385ab, DYS447, DYS449, DYS481, DYS504, DYS518, DYS612, DYS626, DYS644 and DYS710 were studied in 461 males comprising 139 Hausa, 96 Igbo and 226 Yoruba ethnic populations. Sixty alleles were recorded for the pooled dataset across the 10 loci, with allele frequencies ranging from 0.0022 to 0.6052. There were 430 haplotypes detected, 403 unique (singletons), and 27 were shared. The discrimination capacity and haplotype diversity were 0.9330 and 0.9770, respectively. Allelic richness was highest in the locus DYS626 and lowest in DYS504. Private alleles were found in each group, with the Hausa population having the highest number. Duplications and microvariant alleles were also reported. These findings may be helpful for paternal lineage investigations, population genetics, and forensic applications among the Nigerian populations.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 48%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Forensic parameters of 10 Y-STR markers in the Nigerian Hausa, Igbo, and Yoruba populations

Fakorede,

Adekoya,

Iroanya

et al. 2024

USci

View full text Add to dashboard Cite

show abstract

“…Alleles selected for the ladder were based on genotypic and sequence data captured previously in South African and Lesotho populations [15–20].…”

Section: Methodsmentioning

confidence: 99%

Allelic ladder production for a 10 locus Y‐chromosome DNA profiling system

Kasu

Fraser²,

Lesaoana

et al. 2020

Separation Science Plus

View full text Add to dashboard Cite

The allelic ladder component of genotyping systems is of utmost importance for accurate allele designation and sizing precision quality assurances by capillary electrophoresis. The production process for ladders also demands adherence to specific technical criteria in order to pass as a reliable profiling system. The conventional methodology for ladder production includes allele isolation by polyacrylamide gel electrophoresis purification followed by amplification of each allele isolate using the polymerase chain reaction. Alternatively, the allele cloning approach has long been promoted as being less labor intensive, higher throughput, and more suitable for long‐term storage of the allele isolates. In this study, we present a workflow to produce a short tandem repeat allelic ladder using a cloning approach. We identified the key factors that may impact the quality of the ladder and provide recommendations to resolve these challenges. In this study, 143 alleles from 10 Y‐chromosome short tandem repeat loci were cloned and amplified individually from plasmid extracts. Amplicons were assessed using quality assurance criteria for selection of polymerase chain reaction products and dilution factors to produce, first, a balanced ladder for each locus, and then a final ladder compiled from each individual locus ladder. The final product was suitable for forensic applications.

show abstract

“…About 90% of the Burkinabe population speak Moore, Fulfulde and Dioula [1,3]. When genetic markers are applied to forensic cases of a certain population, forensic parameters for the population in question must be known [4]. However, no study on forensic parameters of a 29 Y-STR loci dataset has been done in BF to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

Population data and genetic structure analysis based on 29 Y-STR loci among the ethnolinguistic groups in Burkina Faso

Zeye

Ouédraogo

et al. 2021

Int J Legal Med

View full text Add to dashboard Cite

Burkina Faso (BF) is a landlocked Sahelian country located in the middle of West Africa. Sixty-three local languages are spoken in BF. Despite this high diversity, the BF population remains poorly investigated, and updated forensic parameters with a large number of Y chromosome short tandem repeats (Y-STRs) are still missing. Herein, 447 DNA samples were typed for a cocktail of 29 Y-STR loci. None of these 447 individuals in total shared a common haplotype. The overall Y-STR haplotypes were successfully uploaded online on the Y-STR Haplotype Reference Database (YHRD) with the accession numbers YA004690 and YA004691. The main haplotype diversity was 0.9999999965, which is much higher than that obtained with 12 Y-STRs in a previous study. Haploid Match Probability for the whole dataset was 0.002237. The phylogenetic analysis of 24 ethnic groups of BF shows that the ethnic group named BISSA is closer to Gur speakers than Mande speakers, where they belong. In addition, genetic structure analysis of 49 African subpopulations sheds light on the fact that geographic proximity turns out to be one of the best predictors of genetic affinity between populations.

show abstract

Forensic parameters and genetic structure based on Y-chromosome short tandem repeats in Lesotho populations

Cited by 5 publications

References 6 publications

Forensic parameters of 10 Y-STR markers in the Nigerian Hausa, Igbo, and Yoruba populations

Forensic parameters of 10 Y-STR markers in the Nigerian Hausa, Igbo, and Yoruba populations

Allelic ladder production for a 10 locus Y‐chromosome DNA profiling system

Population data and genetic structure analysis based on 29 Y-STR loci among the ethnolinguistic groups in Burkina Faso

Contact Info

Product

Resources

About