Forensic Soil Characterization

Thornton, John I.; Crim, D.

doi:10.1007/978-3-642-69400-4_1

Cited by 12 publications

(8 citation statements)

References 46 publications

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“…Patented luminol molecules or luminol blood-dependent CL enhancers are used in several luminol preparations [142, 164]. They are thought to improve sensitivity, specificity, and duration of the emission of luminescence.…”

Section: Forensic Sciencementioning

confidence: 99%

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Khan

Idrees

Moxley

et al. 2014

Appl Biochem Biotechnol

238

181

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Chemiluminescence (CL) is an important method for quantification and analysis of various macromolecules. A wide range of CL agents such as luminol, hydrogen peroxide, fluorescein, dioxetanes and derivatives of oxalate, and acridinium dyes are used according to their biological specificity and utility. This review describes the application of luminol chemiluminescence (LCL) in forensic, biomedical, and clinical sciences. LCL is a very useful detection method due to its selectivity, simplicity, low cost, and high sensitivity. LCL has a dynamic range of applications, including quantification and detection of macro and micromolecules such as proteins, carbohydrates, DNA, and RNA. Luminol-based methods are used in environmental monitoring as biosensors, in the pharmaceutical industry for cellular localization and as biological tracers, and in reporter gene-based assays and several other immunoassays. Here, we also provide information about different compounds that may enhance or inhibit the LCL along with the effect of pH and concentration on LCL. This review covers most of the significant information related to the applications of luminol in different fields.

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Section: Forensic Sciencementioning

confidence: 99%

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Khan

Idrees

Moxley

et al. 2014

Appl Biochem Biotechnol

238

181

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“…The main physical techniques normally used for forensic issues are: particle size distribution [2][3][4][5][6][7]; soil color UV-vis www.elsevier.com/locate/forsciint Available online at www.sciencedirect.com spectrum [4,8,9]; density gradient [10]; microscopical features [1]; Fourier Transformed Infrared Spectroscopy (FTIR) [11] and; X-ray diffraction [12].…”

Section: Introductionmentioning

confidence: 99%

Chemical, physical and mineralogical characterization of soils from the Curitiba Metropolitan Region for forensic purpose

Melo

Barbar²,

Zamora

et al. 2008

Forensic Science International

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“…The potential for using the microbial component of soil for forensic comparison was originally identified by Thornton (1986) who recognised that microbes can offer information about the specific local environment that sustains them. More recently, the ability to compare forensic soil samples using molecular bacterial analysis was demonstrated by Horswell et al (2002), but it was recognised that further development and evaluation of the utility and reliability of the technique was required if it was to be used for routine forensic comparison.…”

Section: Forensic Comparisonmentioning

confidence: 99%

“…Bacteria are the most numerous component of the soil microbial population. It has been estimated that there may be as many as 10 9 bacterial cells per gram of soil (Harris, 1994) and that half of the total biomass on earth consists of bacteria located in the top two to three centimetres of the soil (Thornton, 1986). It is widely accepted that the majority of soil bacteria, possibly as many as 99 %, cannot be cultured using traditional laboratory media based techniques (Amann et al, 1995).…”

Section: Soil Biologymentioning

confidence: 99%

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Forensic DNA Profiling of Bacterial Communities in Soil

Parkinson¹

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<p>Soil is frequently encountered as trace evidence in forensic science case-work, but because of the limitations of current analytical techniques, this evidence is rarely utilised. A technique has been developed that allows comparisons of soil samples to be made, based on molecular analysis of the bacterial communities living in the soil. This project assesses the practicality of using this technique, known as 16S rDNA T-RFLP community profiling, for forensic soil analysis, by refining the basic methodology and performing a preliminary evaluation of its reproducibility and utility. Initial difficulties associated with generating profiles from soil samples have been overcome through methodology improvement, and the technique has been found to be effective for generating simple, visual profiles that clearly demonstrate differences between soil samples. Soil bacterial community DNA profiling is likely to be a powerful yet simple forensic tool, providing the ability to routinely use soil as associative evidence. The potential for using the same technology to develop a time since death or post mortem interval (PMI) estimation tool was also investigated. This study monitored the changes in the soil bacterial community beneath decomposing human cadavers and pig carcasses and showed that community change is dynamic and progressive. These changes are caused by fluctuations in specific bacterial species populations that are able to utilise organic breakdown products released from the body over time. Release of the body’s natural microflora into the underlying soil may also contribute to an altered bacterial community. This project has demonstrated that the soil microbial community clearly changes over the course of decomposition, and potential exists for development of a PMI estimation tool based on soil bacterial community succession.</p>

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Forensic Soil Characterization

Cited by 12 publications

References 46 publications

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Chemical, physical and mineralogical characterization of soils from the Curitiba Metropolitan Region for forensic purpose

Forensic DNA Profiling of Bacterial Communities in Soil

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