Forensic Validation of the AmpF^|^#x2113;STR^|^reg; Identifiler^|^reg; Plus PCR Amplification Kit and Comparison of Performance with AmpF^|^#x2113;STR^|^reg; Identifiler^|^reg; PCR Amplification Kit

Abstract

To obtain DNA typing results from forensic biological samples is occasionally di‹cult, since they are complex and diverse. AmpF STRIdentiˆlerPlus PCR Ampliˆcation Kit (IDPlus) was developed to improve the success rate of DNA typing from such samples by Applied Biosystems. Forensic biologists must be cautious in DNA typing when considering the characteristics of the Kit used for analyzing in such samples. Therefore, to reveal to the characteristics of IDPlus Kit is very important to increase the reliability and probative value of DNA typing in the trial and investigation. In this study, IDPlus is evaluated for the characteristics and the DNA detectability. We also compared IDPlus with AmpF STRIdentiˆlerPCR Ampliˆcation Kit (ID) by analyzing samples di‹cult to obtain DNA types. The results from the PCR inhibitor study using bloodstain on soil or denim indicated that IDPlus was more resistant to PCR inhibitors than ID. In the study of analyzing degraded DNA, there were no signiˆcant diŠerences to be found except for the sensitivity. The peak corresponding to insu‹ciently ampliˆed products due to a point mutation on primer binding site was higher using IDPlus than ID in electropherogram. Also the anomalous peaks which were observed in analyzing same mammal animal DNA samples were higher. Hence, IDPlus is considered to be very eŠective leverage to samples analysis which is di‹cult to detect allele due to PCR inhibitors. The primer binding ability to the template DNA was altered when using IDPlus compared with the ID so this should be taken into consideration. Forensic

Non-specific peaks generated by animal DNA during human STR analysis: Peak characteristics and a novel analysis method for mixed human/animal samples

“…Earlier cross-species validation studies reported for the non-specific peaks generated from animal DNA are observed in electropherograms near human amelogenin peaks [9][10][11][12]. The shape and observed position of these non-specific peaks are distinct and can be easily distinguished from other artifacts such as dye blobs and primer dimers.…”