Forest Tree Associated Bacterial Diffusible and Volatile Organic Compounds against Various Phytopathogenic Fungi

Kong, Wei‐Liang; Li, Pu-Sheng; Wu, Xiaoqin; Wu, Tian-Yu; Sun, Xiao‐Rui

doi:10.3390/microorganisms8040590

Cited by 50 publications

(37 citation statements)

References 51 publications

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“…These fungi play an important role in tree growth, soil nutrient cycling, and disease protection. For instance, Trichoderma and Purpureocillium are potential biocontrol agents of plant pathogens [70,71], and some species of Phomopsis promotes the growth and yield of the host plant, reduces fertilization and can be used as a biocontrol agent [72,73]. The naturally grown walnut trees were not treated with chemical fertilizer, and thus the soil around these trees supported a higher fungal diversity, as previously observed by Bossio et al [74] and Xu et al [75].…”

Section: Discussionmentioning

confidence: 62%

Soil Chemical and Microbiological Properties Are Changed by Long-Term Chemical Fertilizers That Limit Ecosystem Functioning

et al. 2020

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Although the effects of fertilization and microbiota on plant growth have been widely studied, our understanding of the chemical fertilizers to alter soil chemical and microbiological properties in woody plants is still limited. The aim of the present study is to investigate the impact of long-term application of chemical fertilizers on chemical and microbiological properties of root-associated soils of walnut trees. The results show that soil organic matter (OM), pHkcl, total nitrogen (TN), nitrate-nitrogen (NO3−), and total phosphorus (TP) contents were significantly higher in non-fertilized soil than after chemical fertilization. The long-term fertilization led to excessive ammonium-nitrogen (NH4+) and available phosphorus (AP) residues in the cultivated soil, among which NH4+ resulted in soil acidification and changes in bacterial community structure, while AP reduced fungal diversity. The naturally grown walnut trees led to an enrichment in beneficial bacteria such as Burkholderia, Nitrospira, Pseudomonas, and Candidatus_Solibacter, as well as fungi, including Trichoderma, Lophiostoma, Phomopsis, Ilyonectria, Purpureocillium, Cylindrocladiella, Hyalorbilia, Chaetomium, and Trichoglossum. The presence of these bacterial and fungal genera that have been associated with nutrient mobilization and plant growth was likely related to the higher soil OM, TN, NO3−, and TP contents in the non-fertilized plots. These findings highlight that reduced chemical fertilizers and organic cultivation with beneficial microbiota could be used to improve economic efficiency and benefit the environment in sustainable agriculture.

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Section: Discussionmentioning

confidence: 62%

Soil Chemical and Microbiological Properties Are Changed by Long-Term Chemical Fertilizers That Limit Ecosystem Functioning

et al. 2020

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“…To determine the inhibition ability of the endophytes isolated from P. polyphylla against wolfberry pathogens, the two‐sealed‐base‐plates method [18,19] was applied. Next, approximately 100 μl of the bacterial suspension was cultivated at 37°C for 10 h and then aspirated and uniformly spread onto the LB solid medium.…”

Section: Methodsmentioning

confidence: 99%

“…To determine the inhibition ability of the endophytes isolated from P. polyphylla against wolfberry pathogens, the two-sealed-base-plates method [18,19] was applied.…”

Section: Identification Of a Highly Antifungal Bacterial Endophytementioning

confidence: 99%

The inhibitory effect of volatile organic compounds produced by Bacillus subtilis CL2 on pathogenic fungi of wolfberry

Ling

Zhao

et al. 2020

J Basic Microbiol

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Bacillus subtilis strain CL2 is antagonistic to wolfberry postharvest pathogenic fungi. In this study, we isolated and screened this strain for in vitro experiments. The result of the two-sealed-base-plates method revealed that volatile organic compounds (VOCs) emitted from the strain CL2 inhibited the hyphal growth of four pathogenic fungi Mucor circinelloides LB1, Fusarium arcuatisporum LB5, Alternaria iridiaustralis LB7, and Colletotrichum fioriniae LB8. After exposure to VOCs for 5 days, the hyphal growth of the pathogen C. fioriniae LB8 was inhibited by 73%. Scanning electron microscopy revealed that the VOCs produced by B. subtilis CL2 caused the mycelium morphology of the pathogenic fungi to deform, twist, fold, and shrink. In the in vivo experiments, we noticed that VOCs could significantly reduce the weight loss rate of wolfberry fruits caused by the pathogenic fungus M. circinelloides LB1 and that the decay incidence rate were caused by the pathogenic fungi F. arcuatisporum LB5, A. iridiaustralis LB7, and C. fioriniae LB8. On the basis of the headspace-gas chromatography-ion mobility spectrometry analysis, seven VOCs produced by strain CL2 were identified. Among them, 2,3-butanedione and 3-methylbutyric acid are the main antifungal active substances. This study investigated the antifungal properties of VOCs produced by the strain CL2 on postharvest pathogenic fungi isolated from wolfberry fruits both in vivo and in vitro, thereby providing the theoretical basis for its future applications. K E Y W O R D S antifungal activity, Bacillus subtilis CL2, HS-GC-IMS, volatile organic compounds (VOCs), wolfberry 1 | INTRODUCTION Wolfberry (Lycium barbarum. L) is a deciduous shrub plant belonging to the family Solanaceae, which is also known as Goji Berry [1]. Being the conventional Chinese medicinal herb for over 2000 years now, wolfberry fruits contain a variety of bioactive substances such as L. barbarum polysaccharide [2], carotenoids, betaine, vitamins, and cerebroside bioactive substances that have now been recognized as the latest "superfruit" in the world [3]. Because

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“…There was a total of 18 volatiles identified in the LAM7/MR-VP BVC blend and consisted of, amongst others, 3-methyl-1-butanol, 1-decanol, 1-dodecanol, hexadecane, 1-octanol and 1-tridecanol which were also identified as growth promoters in previous studies ( Velázquez-Becerra et al, 2011 ; Bee Park et al, 2013 ; Velivelli et al, 2015 ; Pérez-Flores et al, 2017 ; Camarena-Pozos et al, 2019 ). In BRP 14/MR-VP the predominant BVC, from a total blend of just five, was identified as 1-undecene, a particularly potent volatile in the biocontrol effect against some fungal phytopathogens mediated by many Pseudomonas strains ( Kong et al, 2020 ). The greatest effect on stem length was observed with plants exposed to BVCs of BRP14/MR-VP.…”

Section: Discussionmentioning

confidence: 99%

Volatile Compounds From Bacillus, Serratia, and Pseudomonas Promote Growth and Alter the Transcriptional Landscape of Solanum tuberosum in a Passively Ventilated Growth System

et al. 2021

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The interaction of an array of volatile organic compounds (VOCs) termed bacterial volatile compounds (BVCs) with plants is now a major area of study under the umbrella of plant-microbe interactions. Many growth systems have been developed to determine the nature of these interactions in vitro. However, each of these systems have their benefits and drawbacks with respect to one another and can greatly influence the end-point interpretation of the BVC effect on plant physiology. To address the need for novel growth systems in BVC-plant interactions, our study investigated the use of a passively ventilated growth system, made possible via Microbox® growth chambers, to determine the effect of BVCs emitted by six bacterial isolates from the genera Bacillus, Serratia, and Pseudomonas. Solid-phase microextraction GC/MS was utilized to determine the BVC profile of each bacterial isolate when cultured in three different growth media each with varying carbon content. 66 BVCs were identified in total, with alcohols and alkanes being the most abundant. When cultured in tryptic soy broth, all six isolates were capable of producing 2,5-dimethylpyrazine, however BVC emission associated with this media were deemed to have negative effects on plant growth. The two remaining media types, namely Methyl Red-Voges Proskeur (MR-VP) and Murashige and Skoog (M + S), were selected for bacterial growth in co-cultivation experiments with Solanum tuberosum L. cv. ‘Golden Wonder.’ The BVC emissions of Bacillus and Serratia isolates cultured on MR-VP induced alterations in the transcriptional landscape of potato across all treatments with 956 significantly differentially expressed genes. This study has yielded interesting results which indicate that BVCs may not always broadly upregulate expression of defense genes and this may be due to choice of plant-bacteria co-cultivation apparatus, bacterial growth media and/or strain, or likely, a complex interaction between these factors. The multifactorial complexities of observed effects of BVCs on target organisms, while intensely studied in recent years, need to be further elucidated before the translation of lab to open-field applications can be fully realized.

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Forest Tree Associated Bacterial Diffusible and Volatile Organic Compounds against Various Phytopathogenic Fungi

Cited by 50 publications

References 51 publications

Soil Chemical and Microbiological Properties Are Changed by Long-Term Chemical Fertilizers That Limit Ecosystem Functioning

Soil Chemical and Microbiological Properties Are Changed by Long-Term Chemical Fertilizers That Limit Ecosystem Functioning

The inhibitory effect of volatile organic compounds produced by Bacillus subtilis CL2 on pathogenic fungi of wolfberry

Volatile Compounds From Bacillus, Serratia, and Pseudomonas Promote Growth and Alter the Transcriptional Landscape of Solanum tuberosum in a Passively Ventilated Growth System

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