Foreword by Lars Nordberg

Nordberg, Lars

doi:10.1016/s0269-7491(98)80006-7

Environmental Pollution

1998

DOI: 10.1016/s0269-7491(98)80006-7

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Foreword by Lars Nordberg

Lars Nordberg¹

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2000

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“…Moreover, the International Agency for Research on Cancer (IARC) considers that cadmium and cadmium compounds are potentially carcinogenic to human (Group 1) [3,4].Metallothioneins (MT) play an important role for heavy metal detoxification, zinc and copper homeostasis, protection against oxidative damage caused by free radicals and as part of the acute phase response to inflammation and stress.The isoforms of MTs have structural similarity with the same number of cysteine residues and high metal binding affinity but differ in their total charge because of differences in certain amino acids other than cysteine.The polymorphism occurs during the evolution of the species and consists of the variation of the primary structure by the substitution of 1 to 15 amino acids. Hence, isoforms differ in amino acid composition and consequently, have different isoelectric points and different hydrophobicities.…”

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Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography

Ferrarello¹,

Campa²,

Infante³

et al. 2000

Analusis

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Over the last decade, it has been documented that cadmium exposure can cause several health disorders including nephrotoxicity, hypertension, osteomalacia, lung disease and disturbed calcium metabolism [1,2]. Moreover, the International Agency for Research on Cancer (IARC) considers that cadmium and cadmium compounds are potentially carcinogenic to human (Group 1) [3,4].Metallothioneins (MT) play an important role for heavy metal detoxification, zinc and copper homeostasis, protection against oxidative damage caused by free radicals and as part of the acute phase response to inflammation and stress.The isoforms of MTs have structural similarity with the same number of cysteine residues and high metal binding affinity but differ in their total charge because of differences in certain amino acids other than cysteine.The polymorphism occurs during the evolution of the species and consists of the variation of the primary structure by the substitution of 1 to 15 amino acids. Hence, isoforms differ in amino acid composition and consequently, have different isoelectric points and different hydrophobicities.Isoforms with minor differences, such as one amino acid residue, were detected as subgroups of the major isoforms and are termed sub-isoforms [5]. It has been demonstrated that the different isoforms may be regulated by different inducers [6]. Experiments performed on cultured rabbit kidney cell [7] and human cell lines [8] demonstrated that various MT isoforms can be synthesised by distinct inducing factors and may play different cellular roles.Thus, the development of analytical methods for reliable measurement of the different isoforms of MTs, within a reasonable time, is nowadays urgently needed for many studies requiring MT isoforms information. Similarly, information about binding of toxic metals, e.g. Cd, to such MTs isoforms is desirable. That is, cadmium speciation studies in biological materials should be carried out [9]. Probably the coupling a powerful separation technique (e.g. chromatographic techniques) with an element specific detector (atomic detector) is the most popular approach for speciation purposes. For unknown species, electrospray ionisation MS studies are opening new exciting possibilities for a more detailed characterisation of metallothioneins in terms of their molecular mass [10].The use of a quadrupole ICP-MS detector coupled on-line with liquid chromatography is one of the most powerful modern tools for studying protein binding biometals in biological fluids. Thus, in our laboratory the determination of Cd-MT in human urine has been carried out by using a Protein Pak DEAE 5PW anion-exchange column coupled to ICP-MS via a vesicular hydride generation interface [11]. This hybrid technique was assessed for the cadmium speciation in standards of rabbit liver MT. However, such methodology has shown to provide poor resolution and reproducibility and rather long separation time. Therefore, the main objective of the present study has been to investigate the potential use of Fast Protein Liquid ...

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confidence: 99%

“…Moreover, the International Agency for Research on Cancer (IARC) considers that cadmium and cadmium compounds are potentially carcinogenic to human (Group 1) [3,4].…”

mentioning

confidence: 99%

Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography

Ferrarello¹,

Campa²,

Infante³

et al. 2000

Analusis

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show abstract

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Foreword by Lars Nordberg

Cited by 1 publication

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Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography

Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography

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