Forks and combs and DNA: the synthesis of branched oligodeoxyribonucleotides

Horn, Thomas; Urdea, Mickey S.

doi:10.1093/nar/17.17.6959

Cited by 79 publications

(62 citation statements)

References 18 publications

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“…Polymeric matrices allow the attachment of multiple SACs, making them an attractive platform for generating polyvalent substrate molecules. Different types of polymeric matrices comprised of DNA itself have been described (20,27,28). These polymeric or dendritic DNA structures have been used to amplify radioactive or fluorescent signals by direct incorporation of label in hybridization-based detection (28)(29)(30).…”

Section: Resultsmentioning

confidence: 99%

Signal amplification through nucleotide extension and excision on a dendritic DNA platform

Capaldi

Getts²,

Jayasena³

2000

Nucleic Acids Research

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Techniques that provide strong signal amplification are useful in diagnostic applications, especially in detecting low concentrations of non-amplifiable target molecules. A versatile and strong signal amplification method based on activities of a DNA polymerase to generate high concentrations of pyrophosphate (PPi) is described. The generation of PPi is catalyzed by nucleotide extension and excision activities of a DNA polymerase on an oligonucleotide cassette. The signal is generated upon enzymatic conversion of PPi to ATP and ATP levels subsequently detected with firefly luciferase. Bioluminesence produced by an oligonucleotide cassette consisting of just two polymerase reaction sites is sufficient to detect them at low attomole levels. The attachment of a large number of these oligonucleotide cassettes to DNA dendrimers enabled the detection of such polyvalent substrate molecules at low zeptomole (10 -21 mol) concentrations. The extent of signal amplification obtained with dendrimer substrates is comparable to exponential target amplifications provided by nucleic acid amplification methods. The attachment of such PPi-generating dendritic DNA platforms to ligands that mediate target recognition would potentially permit detection of extremely low concentrations of analytes in diagnostic assays.

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Section: Resultsmentioning

confidence: 99%

Signal amplification through nucleotide extension and excision on a dendritic DNA platform

Capaldi

Getts²,

Jayasena³

2000

Nucleic Acids Research

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“…The secondary sequences of the synthesized oligonucleotide serve as hybridization sites for an enzyme-labeled oligonucleotide. The primary and secondary sequences are covalently linked through branch points (39). Signal amplification results from the attachment of 60 to 300 enzyme molecules (92) to each nucleic acid target (Fig.…”

Section: Signal Amplification and Detection Methodsmentioning

confidence: 99%

Advances in nucleic acid-based detection methods

Wolcott

1992

Clin Microbiol Rev

153

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Laboratory techniques based on nucleic acid methods have increased in popularity over the last decade with clinical microbiologists and other laboratory scientists who are concerned with the diagnosis of infectious agents. This increase in popularity is a result primarily of advances made in nucleic acid amplification and detection techniques. Polymerase chain reaction, the original nucleic acid amplification technique, changed the way many people viewed and used nucleic acid techniques in clinical settings. After the potential of polymerase chain reaction became apparent, other methods of nucleic acid amplification and detection were developed. These alternative nucleic acid amplification methods may become serious contenders for application to routine laboratory analyses. This review presents some background information on nucleic acid analyses that might be used in clinical and anatomical laboratories and describes some recent advances in the amplification and detection of nucleic acids.

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“…Molecules of different shapes and topology, such as cubes and knots, 1,2 DNA combs, 3 and oligonucleotide dendrimers, 4,5 have been described. Self-assembling multistranded complexes of DNA oligonucleotides constitute a special class of DNA superstructures.…”

Section: Introductionmentioning

confidence: 99%