Formation and Characterization of Fluid Lipid Bilayers on Alumina

Mager, Morgan; Almquist, Benjamin D.; Melosh, Nicholas A.

doi:10.1021/la802726u

Cited by 56 publications

(81 citation statements)

References 34 publications

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“…Figure 3B presents normalized fluorescence intensity traces of the bleached spot and the mobile fraction was ∼86%, which is also in agreement with previous measurements. 42 The calculated diffusion coefficient and mobile fraction are lower than those which are typically obtained on silicon oxide, 42 and this difference has been attributed to stronger hydrodynamic coupling. Previous NMR studies on oxide nanoparticles also indicate that water near an aluminum oxide surface is less mobile than that near a silicon oxide surface.…”

Section: Acs Applied Materials and Interfacesmentioning

confidence: 91%

Self-Assembly Formation of Lipid Bilayer Coatings on Bare Aluminum Oxide: Overcoming the Force of Interfacial Water

Jackman

Tabaei

Zhao

et al. 2014

ACS Appl. Mater. Interfaces

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Widely used in catalysis and biosensing applications, aluminum oxide has become popular for surface functionalization with biological macromolecules, including lipid bilayer coatings. However, it is difficult to form supported lipid bilayers on aluminum oxide, and current methods require covalent surface modification, which masks the interfacial properties of aluminum oxide, and/or complex fabrication techniques with specific conditions. Herein, we addressed this issue by identifying simple and robust strategies to form fluidic lipid bilayers on aluminum oxide. The fabrication of a single lipid bilayer coating was achieved by two methods, vesicle fusion under acidic conditions and solvent-assisted lipid bilayer (SALB) formation under near-physiological pH conditions. Importantly, quartz crystal microbalance with dissipation (QCM-D) monitoring measurements determined that the hydration layer of a supported lipid bilayer on aluminum oxide is appreciably thicker than that of a bilayer on silicon oxide. Fluorescence recovery after photobleaching (FRAP) analysis indicated that the diffusion coefficient of lateral lipid mobility was up to 3-fold greater on silicon oxide than on aluminum oxide. In spite of this hydrodynamic coupling, the diffusion coefficient on aluminum oxide, but not silicon oxide, was sensitive to the ionic strength condition. Extended-DLVO model calculations estimated the thermodynamics of lipid-substrate interactions on aluminum oxide and silicon oxide, and predict that the range of the repulsive hydration force is greater on aluminum oxide, which in turn leads to an increased equilibrium separation distance. Hence, while a strong hydration force likely contributes to the difficulty of bilayer fabrication on aluminum oxide, it also confers advantages by stabilizing lipid bilayers with thicker hydration layers due to confined interfacial water. Such knowledge provides the basis for improved surface functionalization strategies on aluminum oxide, underscoring the practical importance of surface hydration.

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Section: Acs Applied Materials and Interfacesmentioning

confidence: 91%

Self-Assembly Formation of Lipid Bilayer Coatings on Bare Aluminum Oxide: Overcoming the Force of Interfacial Water

Jackman

Tabaei

Zhao

et al. 2014

ACS Appl. Mater. Interfaces

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“…Progress has been made to form SLBs on many solid non-siliceous surfaces including chrome,[81] indium tin oxide,[82] gold,[53*] titanium oxide,[83] and alumina. [84] In many cases these surfaces have vesicle-substrate interactions that do not enable conventional vesicle fusion, and thus surface-specific techniques must be used to promote SLB formation.…”

Section: Creating Slbs On Non-siliceous Surfacesmentioning

confidence: 99%

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

Hardy

Nayak

Zauscher

2013

Current Opinion in Colloid & Interface Science

193

183

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Vesicle fusion has long provided an easy and reliable method to form supported lipid bilayers (SLBs) from simple, zwitterionic vesicles on siliceous substrates. However, for complex compositions, such as vesicles with high cholesterol content and multiple lipid types, the energy barrier for the vesicle-to-bilayer transition is increased or the required vesicle-vesicle and vesicle-substrate interactions are insufficient for vesicle fusion. Thus, for vesicle compositions that more accurately mimic native membranes, vesicle fusion often fails to form SLBs. In this paper, we review three approaches to overcome these barriers to form complex, biomimetic SLBs via vesicle fusion: (i) optimization of experimental conditions (e.g., temperature, buffer ionic strength, osmotic stress, cation valency, and buffer pH), (ii) α-helical (AH) peptide-induced vesicle fusion, and (iii) bilayer edge-induced vesicle fusion. AH peptide-induced vesicle fusion can form complex SLBs on multiple substrate types without the use of additional equipment. Bilayer edge-induced vesicle fusion uses microfluidics to form SLBs from vesicles with complex composition, including vesicles derived from native cell membranes. Collectively, this review introduces vesicle fusion techniques that can be generalized for many biomimetic vesicle compositions and many substrate types, and thus will aid efforts to reliably create complex SLB platforms on a range of substrates.

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“…Lipid vesicles can rupture to form planar bilayers on hydrophilic surfaces such as glass, mica, and silicon oxide [11]. By contrast, vesicles typically absorb and do not rupture on other hydrophilic surfaces such as gold, titanium oxide, and aluminum oxide [12][13][14]. Hence, the latter substrates can be employed to spontaneously form adsorbed vesicle layers.…”

Section: Introductionmentioning

confidence: 99%

Rupture of zwitterionic lipid vesicles by an amphipathic, α-helical peptide: Indirect effects of sensor surface and implications for experimental analysis

Zan¹,

Cho²

2014

Colloids and Surfaces B: Biointerfaces

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Formation and Characterization of Fluid Lipid Bilayers on Alumina

Cited by 56 publications

References 34 publications

Self-Assembly Formation of Lipid Bilayer Coatings on Bare Aluminum Oxide: Overcoming the Force of Interfacial Water

Self-Assembly Formation of Lipid Bilayer Coatings on Bare Aluminum Oxide: Overcoming the Force of Interfacial Water

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

Rupture of zwitterionic lipid vesicles by an amphipathic, α-helical peptide: Indirect effects of sensor surface and implications for experimental analysis

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