Formation and Electrical Evaluation of a Single Metallized DNA Nanowire in a Nanochannel

Abstract

A novel fabrication process was developed for a single silver nanowire using DNA metallization in a nanochannel, and the electrical properties of this nanowire were evaluated using electrochemical impedance spectroscopy. After being isolated using a nanochannel measuring 500 nm in depth and 500 nm in width, a single λDNA molecule was electrostatically stretched and immobilized between two electrodes separated by a gap of 15 μm by applying an AC voltage of 1 MHz and 20 Vp‐p. Then, naphthalene diimide molecules terminally‐labeled with galactose moieties were intercalated into the λDNA, and the reduction of silver ions along the λDNA led to its metallization with silver. Scanning electron microscopy observations revealed that two nanowires having different average widths of 154 nm and 250 nm were formed in two individual nanochannels. The nanowires showed the linear current‐voltage characteristics, and their combined resistance was estimated to be 45.5 Ω. The complex impedance of the nanowires was measured, and an equivalent circuit was obtained as a series connection of a resistance and a parallel resistance‐constant phase element circuit. Impedance analysis revealed that the nanowire included silver grain boundaries, and the bulk resistivity of silver grain was estimated to be 8.35×10−8 Ωm.

Functional Naphthalene Diimides: Synthesis, Properties, and Applications

“…This property was then used to evaluate the feasibility of electrochemical detection of the PCR product derived from aberrant methylation of CDH 4 , which is associated with the development of colon cancer (Figure 98 ). 410 Himuro et al 411 used a similar approach for fabrication of a single metallized DNA nanowire in a nanochannel. The λDNA molecule was electrostatically stretched and immobilized between two electrodes with a 15 μm gap by applying an AC voltage of 1 MHz and 20 V p-p .…”

Review of the Electrical Characterization of Metallic Nanowires on DNA Templates

“…The NDI intercalated in the ds-DNA, leaving the galactoses as reducing agents. At the last step, silver ions were injected and aldehyde group of the galactoses reduced the silver ions along DNA [ 23 ].…”

“…Electrical characterization of nanowires has been performed by either contacting them directly with electrodes [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ] or using scanning probe microscopy [ 25 , 26 , 27 , 28 ]. In most measurements, contact resistances dominated over the contribution from the resistance along the wire, and careful studies of the conductance of the wires indicated that the resistivity is higher than purely metallic resistivity.…”

Considerations on triangular‐shaped electrodes for deoxyribonuclease detection

“…[1][2][3] DNA is known to pass electric current under certain conditions, 4 and a number of research groups reported on evaluation of DNA electric characteristics. [5][6][7][8] In addition, a device was proposed to detect deoxyribonuclease (DNase) by measuring impedance change in λDNA stretched and immobilized between opposing triangularshaped electrodes. 9 DNase, a protein present in blood and other body fluids, is deeply involved in DNA metabolism in cells, thus playing an important role in living organisms.…”

“…DNA can form various nanoscale structures; recently, many a research has been conducted on biosensors using DNA 1–3 . DNA is known to pass electric current under certain conditions, 4 and a number of research groups reported on evaluation of DNA electric characteristics 5–8 . In addition, a device was proposed to detect deoxyribonuclease (DNase) by measuring impedance change in λDNA stretched and immobilized between opposing triangular‐shaped electrodes 9 …”