Formation and Origin of Basal Lamina and Anchoring Fibrils in Adult Human Skin

Briggaman, Robert A.; Dalldorf, Frederic G.; Wheeler, Clayton E.

doi:10.1083/jcb.51.2.384

Cited by 204 publications

(58 citation statements)

References 19 publications

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“…Classic studies using epidermal explants, viable and nonviable dermis, and chorioallantoic membrane concluded that the basal lamina is of epithelial origin (Briggaman et al, 1971). Another more recent study using human keratinocytes transplanted to athymic mice concluded that anchoring fibrils are of epithelial origin (Regauer et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Cellular origin of the dermal‐epidermal basement membrane

Marinkovich

Keene

Rimberg

et al. 1993

Developmental Dynamics

231

158

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The basement membrane underlying epithelium of skin is generally believed to be of epithelial origin, but a mesenchymal contribution to the basement membrane has not been directly examined. The purpose of this study was to directly evaluate both epithelial and mesenchyma1 contributions to the basement membrane. Fetal bovine keratinocytes cultured on the surface of collagen gels in the absence of fibroblasts did not produce an ultrastructurally recognizable basement membrane; however, when these cells were cultured in the presence of dermal fibroblasts a basement membrane at the keratinocyte-fibroblast interface was produced after 1 week which was very similar in biochemical composition and ultrastructural appearance to dermal-epidermal basement membrane in human skin. When dual species cultures of bovine keratinocytes and human fibroblasts were analyzed by indirect immunofluorescent microscopy (IF), with human specific antibodies against basement membrane components, dermal fibroblasts were shown to synthesize and deposit type IV collagen, type VII collagen, and laminin in a linear manner into the basement membrane zone. Fetal bovine keratinocytes cultured in the presence or absence of fibroblasts synthesized and deposited type IV collagen, type VII collagen, laminin, K-laminin, kalinin, and basement membrane associated heparan sulfate proteoglycan (HSPG) into the underlying basement membrane zone. In organ culture, a subpopulation of fibroblasts initially migrating from human foreskin explants was found to stain strongly for types VII and IV collagen and laminin by IF whereas after subculture all cells showed a uniform low staining. Based on these observations we propose that differentiated fibroblasts exist adjacent to epithelial tissues in vivo which produce basement membrane components and assist in basement membrane assembly. 0 1993 Wiley-Liss, Inc.

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Section: Introductionmentioning

confidence: 99%

Cellular origin of the dermal‐epidermal basement membrane

Marinkovich

Keene

Rimberg

et al. 1993

Developmental Dynamics

231

158

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“…Ultrastructurally, this basement membrane consists of an electron-lucent region, the lamina lucida, directly adject to the epithelial and myoepithelial cells, and an electron dense region, the lamina densa, located between the lamina lucida and the connective tissue (2). In some tissues anchoring fibrils that extend from the lamina densa into the surrounding connective tissue have been described (3). Several protein components of basement membranes have been identified (4).…”

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confidence: 99%

Topographical arrangement of basement membrane proteins in lactating rat mammary gland: comparison of the distribution of type IV collagen, laminin, fibronectin, and Thy-1 at the ultrastructural level.

Monaghan¹,

Warburton²,

Perusinghe³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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The topographical distribution of type IV collagen, laminin, fibronectin, and the thymocyte differentiation antigen Thy-i in the basement membrane of the lactating rat mammary gland was investigated. Small cubes of tissue, which had not been subjected to prior fixation or freezing, were incubated with monospecific or monoclonal antibodies to these proteins, and the antibodies were located by an indirect immunoperoxidase staining technique and observed in the electron microscope. The lamina densa stained uniformly with antibodies to type IV collagen and laminin. In addition, both laminin and type IV collagen were present in semiperiodic clusters that traversed the lamina lucida from the cell surface to the lamina densa. Fibronectin was present only in the semiperiodic clusters and not elsewhere in the basement membrane. These clusters were irregularly spaced along the cell surface and heterogeneous in size. It remains to be determined if these three proteins are present in the same clusters. Thy-I was largely present on the lamina densa and not on the lamina lucida.The Thy-i staining of the lamina densa occurred in discrete maxima and minima. These maxima occurred in regions adjacent to Thy-l-bearing stromal cells. Thus, the topographical distribution of proteins within a basement membrane varies in a nonrandom manner, and local factors can modify this distribution. We suggest that this topographical variability may play a role in cell recognition and signalling processes that occur across the basement membrane.In the rodent and human mammary glands, the parenchyma are separated from the surrounding connective tissue by a basement membrane (1). Ultrastructurally, this basement membrane consists of an electron-lucent region, the lamina lucida, directly adject to the epithelial and myoepithelial cells, and an electron dense region, the lamina densa, located between the lamina lucida and the connective tissue (2). In some tissues anchoring fibrils that extend from the lamina densa into the surrounding connective tissue have been described (3). Several protein components of basement membranes have been identified (4). Type IV collagen appears to be restricted to the lamina densa (5-7), whereas laminin, fibronectin, and heparan sulfate proteoglycan are present in either the lamina lucida or lamina densa (5, 7-12). We have shown that antibodies to type IV collagen, laminin, and the thymocyte differentiation antigen Thy-1 stain the basement membrane in the mammary glands of both virgin and lactating rats at the light microscope level, whereas antibodies to fibronectin only stain the basement membrane in the mammary gland of lactating rats (1,13,14). We now report on the immunocytochemical distribution at the ultrastructural level of type IV collagen, laminin, fibronectin, and Thy-i in the basement membrane of the lactating rat mammary gland. METHODSAntibodies. Monospecific antibodies to type IV collagen and laminin, both purified from the mouse Engelbreth-Holm-Swarm (EHS) sarcoma, and rat plasma fibronectin hav...

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“…the epidermal basement membranes are thicker than Schwann cell basement membranes ( fig. 1a, e), and the former have anchoring filaments [Beerens et al, 1975;Cohn et al, 1977] and anchoring fibrils [Briggaman et al, 1971;Scaletta and MacCallum, 1974;Wasano and Yamamoto, 1985], which connect the lamina densa to the epithelial cells and underlying connective tissue, respectively. On the other hand, no distinct connecting structures are observed along the basement membranes of Schwann cells.…”

Section: Discussionmentioning

confidence: 99%

Regeneration of Mouse Lip Epidermis after Cryo Treatment

Osawa¹,

Abe²,

Wang³

et al. 2000

Cells Tissues Organs

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The processes of degeneration and the regeneration of the lip epidermal cells was observed by electron microscopy, focussing on the substance and the structure of the lamina lucida, on which regenerating cells migrated. After the repetitive freezing and thawing treatment, epidermal cells degenerated and detached from the dermis. The separation occurred between the epidermal cells and the basement membrane, leaving a small amount of cell debris on the lamina densa. After the separation of the epidermis, there were some thick parts in the lamina densa which appeared to be the part below hemidesmosomes. Regenerating epidermal cells migrated from the nondegenerated area along the cellular surface of the old lamina densa. They migrated over the cell debris which was gradually phagocitized, and formed new hemidesmosomes with the old lamina densa. Regenerating epidermal cells did not make close contact with the old lamina densa during their migration, but there was a clear space in between, indicating that some of the materials and the structure of the lamina lucida of the old basement membrane was preserved. By immunoelectron microscopy using anti-HSPG (heparan sulfate proteoglycan) antibody, it became clear that after the epidermal separation, HSPG was preserved in the basement membrane to some extent, especially in the thick parts of the lamina densa located below. The immunoelectron micrographs support the view that hemidesmosomes may reform at the previous locations at the old lamina densa.

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Formation and Origin of Basal Lamina and Anchoring Fibrils in Adult Human Skin

Cited by 204 publications

References 19 publications

Cellular origin of the dermal‐epidermal basement membrane

Cellular origin of the dermal‐epidermal basement membrane

Topographical arrangement of basement membrane proteins in lactating rat mammary gland: comparison of the distribution of type IV collagen, laminin, fibronectin, and Thy-1 at the ultrastructural level.

Regeneration of Mouse Lip Epidermis after Cryo Treatment

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