Formation and properties of the activator of plasminogen and of human and bovine plasmin

The Maintenance of a Sustained Thrombolytic State in Man. I. Induction and Effects*

“…The activation of human plasminogen by streptokinase takes place by a two stage reaction (24,25). First streptokinase reacts with a plasma component (termed proactivator) and the resulting activator reacts with plasminogen enzymati- cally.…”

THE INHIBITION OF PLASMIN, PLASMA KALLIKREIN, PLASMA PERMEABILITY FACTOR, AND THE C'1r SUBCOMPONENT OF THE FIRST COMPONENT OF COMPLEMENT BY SERUM C'1 ESTERASE INHIBITOR

“…Casein (I:l~mmersten quality, Nutritional Biochemicals Corp.), was prepared as a 1.6% solution in 0.15 ~ sodium phosphate buffer (pH 7.5) by a modification of the method of Mullertz (20).…”

“…The effect of C'1 esterase inhibitor upon proteolysis by plasmin was measured by a modification of the method of Remmert and Cohen (20,23). 0.5 ml of spontaneously activated human plasmin and 0.5 ml C'1 esterase inhibitor (or 0.005 ~r phosphate buffer) were incubated together at room temperature for 3 rain.…”

Reticuloendothelial Clearance of Circulating Fibrin in the Pathogenesis of the Generalized Shwartzman Reaction

“…Proteolytic activity was determined by the digestion of casein using the method of MuUertz (16). Fibrinolytic activity was estimated by the standard fibrin plate method of Astrup and MuUertz (17), and by determination of the lysis time of sterile whole blood clots and of euglobulin dots.…”