Formation and separation of root border cells

Driouich, Azeddine; Durand, Caroline; Vicré-Gibouin, Maïté

doi:10.1016/j.tplants.2006.11.003

Cited by 95 publications

(78 citation statements)

References 51 publications

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“…Such cells have also been observed in other Brassicaceae species and in flax, an economically important crop in the Linaceae family (Driouich et al, 2007. Here, we further characterize flax border-like cells.…”

Section: Characterization Of Flax Root Border-like Cells and Their Cesupporting

confidence: 55%

“…they remain attached to each other instead of being isolated as individual cells) and number (a few hundreds instead of thousands; Vicré et al, 2005;Durand et al, 2009;Driouich et al, 2012). Root border-like cells are produced by other Brassicaceae species including Brassica napus and radish (Raphanus sativus; Driouich et al, 2007Driouich et al, , 2010Driouich et al, , 2012, although they are not specific to the Brassicaceae family. Indeed, it has been recently shown that border-like cells are also produced by a few other species, including flax (Linum usitatissimum), an agronomically important crop that belongs to the Linaceae family .…”

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confidence: 99%

“…While the protective function of "classical" border cells has been mostly studied in pea (Hawes et al, 2000;Gunawardena and Hawes, 2002;Pan et al, 2004), there is no information available so far regarding the function of root border-like cells in either Arabidopsis or flax plants (Vicré et al, 2005;Driouich et al, 2007Driouich et al, , 2010. Considering the importance of classical root border cells in root protection and plant defense, we hypothesized that border-like cells could also play a significant role in plant-microbe interactions.…”

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confidence: 99%

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Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors

et al. 2013

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Plant pathogens including fungi and bacteria cause many of the most serious crop diseases. The plant innate immune response is triggered upon recognition of microbe-associated molecular patterns (MAMPs) such as flagellin22 and peptidoglycan. To date, very little is known of MAMP-mediated responses in roots. Root border cells are cells that originate from root caps and are released individually into the rhizosphere. Root tips of Arabidopsis (Arabidopsis thaliana) and flax (Linum usitatissimum) release cells known as "border-like cells." Whereas root border cells of pea (Pisum sativum) are clearly involved in defense against fungal pathogens, the function of border-like cells remains to be established. In this study, we have investigated the responses of root border-like cells of Arabidopsis and flax to flagellin22 and peptidoglycan. We found that both MAMPs triggered a rapid oxidative burst in root border-like cells of both species. The production of reactive oxygen species was accompanied by modifications in the cell wall distribution of extensin epitopes. Extensins are hydroxyproline-rich glycoproteins that can be cross linked by hydrogen peroxide to enhance the mechanical strength of the cell wall. In addition, both MAMPs also caused deposition of callose, a well-known marker of MAMP-elicited defense. Furthermore, flagellin22 induced the overexpression of genes involved in the plant immune response in root border-like cells of Arabidopsis. Our findings demonstrate that root borderlike cells of flax and Arabidopsis are able to perceive an elicitation and activate defense responses. We also show that cell wall extensin is involved in the innate immunity response of root border-like cells.

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Section: Characterization Of Flax Root Border-like Cells and Their Cesupporting

confidence: 55%

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confidence: 99%

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confidence: 99%

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Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors

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“…In addition, many border cells (i.e. cells of the root cap that have undergone cellular separation but are still attached to the root via a soluble polysaccharide matrix; Driouich et al, 2007) could be observed 1 to 2 mm from the apex (Fig. 5).…”

Section: Resultsmentioning

confidence: 99%

Examination of the Distribution of Arsenic in Hydrated and Fresh Cowpea Roots Using Two- and Three-Dimensional Techniques

et al. 2012

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Arsenic (As) is considered to be the environmental contaminant of greatest concern due to its potential accumulation in the food chain and in humans. Using novel synchrotron-based x-ray fluorescence techniques (including sequential computed tomography), short-term solution culture studies were used to examine the spatial distribution of As in hydrated and fresh roots of cowpea (Vigna unguiculata 'Red Caloona') seedlings exposed to 4 or 20 mm arsenate [As(V)] or 4 or 20 mm arsenite. For plants exposed to As(V), the highest concentrations were observed internally at the root apex (meristem), with As also accumulating in the root border cells and at the endodermis. When exposed to arsenite, the endodermis was again a site of accumulation, although no As was observed in border cells. For As(V), subsequent transfer of seedlings to an As-free solution resulted in a decrease in tissue As concentrations, but growth did not improve. These data suggest that, under our experimental conditions, the accumulation of As causes permanent damage to the meristem. In addition, we suggest that root border cells possibly contribute to the plant's ability to tolerate excess As(V) by accumulating high levels of As and limiting its movement into the root.

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“…Root border cells are known to originate from the root cap meristem, to detach individually and be released within the external environment (Hawes et al, 2003(Hawes et al, , 2012. All plant species produce populations of border cells that vary in number, shape, size and activity (Hawes et al, 2003;Driouich et al, 2007Driouich et al, , 2012Endo et al, 2011;Plancot et al, 2013). Border cells synthesize and secrete a large variety of substances, among which are many defence-related molecules that contribute to root protection (Wen et al, 2007(Wen et al, , 2009Cannesan et al, 2011;Plancot et al, 2013;Driouich et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Root exudate ofSolanum tuberosumis enriched in galactose-containing molecules and impacts the growth ofPectobacterium atrosepticum

Koroney

Plasson

Pawlak

et al. 2016

Ann Bot

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Background and aims Potato (Solanum tuberosum) is an important food crop and is grown worldwide. It is, however, significantly sensitive to a number of soil-borne pathogens that affect roots and tubers, causing considerable economic losses. So far, most research on potato has been dedicated to tubers and hence little attention has been paid to root structure and function.Methods In the present study we characterized root border cells using histochemical staining, immunofluorescence labelling of cell wall polysaccharides epitopes and observation using laser confocal microscopy. The monosaccharide composition of the secreted exudates was determined by gas chromatography of trimethylsilyl methylglycoside derivatives. The effects of root exudates and secreted arabinogalactan proteins on bacterial growth were investigated using in vitro bioassays.Key Results Root exudate from S. tuberosum was highly enriched in galactose-containing molecules including arabinogalactan proteins as major components. Treatment of the root with an elicitor derived from Pectobacterium atrosepticum, a soil-borne pathogen of potato, altered the composition of the exudates and arabinogalactan proteins. We found that the growth of the bacterium in vitro was differentially affected by exudates from elicited and nonelicited roots (i.e. inhibition versus stimulation).Conclusions Taken together, these findings indicate that galactose-containing polymers of potato root exudates play a central role in root-microbe interactions.

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Formation and separation of root border cells

Cited by 95 publications

References 51 publications

Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors

Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors

Examination of the Distribution of Arsenic in Hydrated and Fresh Cowpea Roots Using Two- and Three-Dimensional Techniques

Root exudate ofSolanum tuberosumis enriched in galactose-containing molecules and impacts the growth ofPectobacterium atrosepticum

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