Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation

Sogo, Yu; Ito, Atsuo; Onoguchi, Masahiro; Oyane, Ayako; Tsurushima, Hideo; Ichinose, Noboru

doi:10.1088/1748-6041/2/3/s16

Cited by 40 publications

(29 citation statements)

References 18 publications

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“…Such improvement includes surface modifications to enhance biocompatibility and osteoconductivity with the use of calcium phosphates, TiO 2 −strontium−CaSiO 3 −biopolymer composite, acid-etching, zinc-modified Ca−Si ceramic, and CaTiSiO 5 ceramic [5–12]. Moreover, biologically active molecules such as fibroblast growth factor-2 (FGF-2), bone morphogenetic proteins (BMP), collagen, fibronectin, 1,25-vitamin D 3 , semaphorin 3A, and bisphosphonate are combined with the surface or incorporated into osteoconductive coatings [11, 13–20]. …”

Section: Introductionmentioning

confidence: 99%

“…Apatite is an osteoconductive material that is widely used in orthopedic and maxillofacial surgery [5–7]. The FGF-2−apatite composite layers form during immersion of the screws in infusion fluid-based supersaturated calcium phosphate solutions containing FGF-2 [13, 25, 26]. FGF-2 co-precipitates with calcium phosphate and consequently can be incorporated into the apatite layer.…”

Section: Introductionmentioning

confidence: 99%

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Reducing the risk of impaired bone apposition to titanium screws with the use of fibroblast growth factor-2−apatite composite layer coating

et al. 2017

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BackgroundLoosening of screws is a common problem in orthopedic and maxillofacial surgery. Modifying the implant surface to improve the mechanical strength of screws has been tried and reported. We developed screws coated with fibroblast growth factor-2 (FGF-2)−apatite composite layers. We then showed, in a percutaneous external fixation model, that this composite layer had the ability to hold and release FGF-2 slowly, thereby reducing the risk of pin tract infection of the percutaneous external fixation. The objective of the current study was to clarify the effect of FGF-2−apatite composite layers on titanium screws on bone formation around the screw.MethodsWe analyzed samples of previously performed animal experiments. The screws were coated with FGF-2−apatite composite layers by immersing them in supersaturated calcium phosphate solutions containing FGF-2. Then, the uncoated, apatite-coated, and FGF-2−apatite composite layer-coated screws were implanted percutaneously in rabbits. Finally, using inflammation-free histological sections, we histomorphometrically assessed them for the presence of bone formation. Weibull plot analysis was then applied to the data.ResultsOn average, screws coated with FGF-2−apatite composite layers showed a significantly higher bone apposition rate than the uncoated or apatite-coated screws. Although the difference in the average bone apposition rate was small, the FGF-2−apatite composite layers produced a significant, marked reduction in the incidence of impaired bone formation around the screw compared with the incidence in the absence of FGF-2 (uncoated and apatite-coated screws). The probability of resulting in a bone apposition rate equal to or less than 63.75%, for example, is 3.5 × 10-4 for screws coated with the FGF-2−apatite composite layers versus 0.05 for screws in the absence of FGF-2.ConclusionsFGF-2-apatite composite layer coating significantly reduced the risk of impaired bone apposition to the screw. Thus, it is feasible to use titanium screws coated with FGF-2−apatite composite layers as internal fixation screws to decrease the risk of loosening.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reducing the risk of impaired bone apposition to titanium screws with the use of fibroblast growth factor-2−apatite composite layer coating

et al. 2017

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“…Plasma-sprayed apatite coating has increased the extraction torque of various external fixation pins by 53%–124% compared with that of uncoated pins [10,11]. Other approaches to form an apatite coating include those using supersaturated calcium phosphate (CaP) solutions [15–21]. Conventional supersaturated solution methods have been generally two-step techniques composed of a physicochemical modification step of substrates and an immersion step in a supersaturated CaP solution prepared from chemical reagents [17–19].…”

Section: Introductionmentioning

confidence: 99%

Improved Bonding of Partially Osteomyelitic Bone to Titanium Pins Owing to Biomimetic Coating of Apatite

Mutsuzaki

Sogo

Oyane

et al. 2013

IJMS

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Increased fixation strength of the bone-pin interface is important for inhibiting pin loosening after external fixation. In a previous study, an apatite (Ap) layer was formed on anodically oxidized titanium (Ti) pins by immersing them in an infusion fluid-based supersaturated calcium phosphate solution at 37 °C for 48 h. In the present study, an Ap layer was also successfully formed using a one-step method at 25 °C for 48 h in an infusion fluid-based supersaturated calcium phosphate solution, which is clinically useful due to the immersion temperature. After percutaneous implantation in a proximal tibial metaphysis for four weeks in rabbits (n = 20), the Ti pin coated with the Ap layer showed significantly increased extraction torque compared with that of an uncoated Ti screw even with partial osteomyelitis present, owing to dense bone formation on the Ap layer in the cortical and medullary cavity regions. When the infection status was changed from “no osteomyelitis” to “partial osteomyelitis,” the extraction torque in the Ap group with “partial osteomyelitis” was almost identical to that for “no osteomyelitis” cases. These results suggest that the Ap layer formed by the room temperature process could effectively improve the fixation strength of the Ti pin for external fixation clinically even with partial osteomyelitis present.

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“…13,14 This biomolecule-CaP affinity provides a potentially universal tool to efficiently incorporate within delivery carriers. Indeed, previous results have shown that growth factors, including bone morphogenetic protein 2 (BMP-2), 15 transforming growth factor-beta 1 (TGF-b1), 16 insulin-like growth factor 1 (IGF-1), 17 and fibroblast growth factor 2 (FGF-2), 18 and other therapeutic agents, such as metal ions for antimicrobial purposes, 19,20 can be surface bound during the formation of CaP cements or coprecipitated during growth of CaP coatings in modified simulated body fluids (mSBF), and can achieve sustained release as the biomineral is resorbed without negatively impacting handling and surgical applicability.…”

Section: Introductionmentioning

confidence: 99%

Effects of BMP-12-Releasing Sutures on Achilles Tendon Healing

Chamberlain

Lee

Leiferman

et al. 2015

Tissue Engineering Part A

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Tendon healing is a complex coordinated event orchestrated by numerous biologically active proteins. Unfortunately, tendons have limited regenerative potential and as a result, repair may be protracted months to years. Current treatment strategies do not offer localized delivery of biologically active proteins, which may result in reduced therapeutic efficacy. Surgical sutures coated with nanostructured minerals may provide a potentially universal tool to efficiently incorporate and deliver biologically active proteins directly to the wound. Additionally, previous reports indicated that treatment with bone morphogenetic protein-12 (BMP-12) improved tendon healing. Based on this information, we hypothesized that mineral-coated surgical sutures may be an effective platform for localized BMP-12 delivery to an injured tendon. The objective of this study was, therefore, to elucidate the healing effects of mineral-coated sutures releasing BMP-12 using a rat Achilles healing model. The effects of BMP-12-releasing sutures were also compared with standard BMP-12 delivery methods, including delivery of BMP-12 through collagen sponge or direct injection. Rat Achilles tendons were unilaterally transected and repaired using BMP-12-releasing suture (0, 0.15, 1.5, or 3.0 mg), collagen sponge (0 or 1.5 mg BMP-12), or direct injection (0 or 1.5 mg). By 14 days postinjury, repair with BMP-12-releasing sutures reduced the appearance of adhesions to the tendon and decreased total cell numbers. BMP-12 released from sutures and collagen sponge also tended to improve collagen organization when compared with BMP-12 delivered through injection. Based on these results, the release of a protein from sutures was able to elicit a biological response. Furthermore, BMP-12-releasing sutures modulated tendon healing, and the delivery method dictated the response of the healing tissue to BMP-12.

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Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation

Cited by 40 publications

References 18 publications

Reducing the risk of impaired bone apposition to titanium screws with the use of fibroblast growth factor-2−apatite composite layer coating

Reducing the risk of impaired bone apposition to titanium screws with the use of fibroblast growth factor-2−apatite composite layer coating

Improved Bonding of Partially Osteomyelitic Bone to Titanium Pins Owing to Biomimetic Coating of Apatite

Effects of BMP-12-Releasing Sutures on Achilles Tendon Healing

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